A5064539421- Infant Nutrition and Health
- Breastfeeding Practices and Influences
- Fatty Acid Research and Health
- Neonatal Respiratory Health Research
- SARS-CoV-2 and COVID-19 Research
- Monoclonal and Polyclonal Antibodies Research
- Protein Hydrolysis and Bioactive Peptides
- Blood groups and transfusion
- COVID-19 Impact on Reproduction
- Probiotics and Fermented Foods
- Vaccine Coverage and Hesitancy
- Advanced Proteomics Techniques and Applications
- Glycosylation and Glycoproteins Research
- Immunodeficiency and Autoimmune Disorders
- Diet, Metabolism, and Disease
- Folate and B Vitamins Research
- Pediatric health and respiratory diseases
- Metabolism and Genetic Disorders
- Complement system in diseases
- Biosensors and Analytical Detection
- Mass Spectrometry Techniques and Applications
- Viral gastroenteritis research and epidemiology
- Escherichia coli research studies
- Protease and Inhibitor Mechanisms
- Congenital Diaphragmatic Hernia Studies
Danone (Netherlands)
2025
Amsterdam University Medical Centers
2022-2024
University of Amsterdam
2022-2024
Emma Kinderziekenhuis
2022-2024
Vrije Universiteit Amsterdam
2024
Utrecht University
2017-2024
Netherlands Metabolomics Centre
2020-2024
Wageningen University & Research
2017
Cincinnati Children's Hospital Medical Center
2014-2016
Children's Hospital & Medical Center
2016
Background: Since the outbreak of coronavirus disease 2019 (COVID-19), many put their hopes in rapid availability effective immunizations. Human milk, containing antibodies against syndrome 2 (SARS-CoV-2), may serve as means protection through passive immunization. We aimed to determine presence and pseudovirus neutralization capacity SARS-CoV-2 specific IgA human milk mothers who recovered from COVID-19, effect pasteurization on these antibodies. Methods: This prospective case control study...
Variations in endogenous peptide profiles, functionality, and the enzymes responsible for formation of these peptides human milk are understudied.
Protein N-glycosylation on human milk proteins assists in protecting an infant's health and functions among others as competitive inhibitors of pathogen binding immunomodulators. Due to the individual uniqueness each mother's overall complexity temporal changes protein N-glycosylation, analysis N-glycoproteome requires longitudinal personalized approaches, providing protein- N-site-specific quantitative information. Here, we describe automated platform using hydrophilic-interaction...
Human milk is the most genuine form of personalized nutrition, whereby its nutritional and bioactive constituents support changing needs growing infant. Personalized proteome profiling strategies may provide insights into maternal–infant relationships. Proteins endogenous peptides in human play an important role as nutrients for growth have distinct functionality such immune defense. Comprehensive monitoring all proteinaceous components, including peptides, required to fully understand...
Human milk is a vital biofluid containing myriad of molecular components to ensure an infant's best start at healthy life. One key component human β-casein, protein which not only structural constituent casein micelles but also source bioactive, often antimicrobial, peptides contributing milk's endogenous peptidome. Importantly, post-translational modifications (PTMs) like phosphorylation and glycosylation typically affect the function proteins peptides; however, here our understanding...
Abstract The most abundant immunoglobulin present in the human body is IgA. It has highest concentrations at mucosal lining and biofluids such as milk second class of antibodies serum. We assessed structural diversity clonal repertoire IgA1-containing molecular assemblies longitudinally serum from three donors using a mass spectrometry-based approach. IgA-containing molecules purified or were by release subsequent analysis their Fab fragments. Our data revealed that IgA1 consists two...
Serum proteomics has matured and is now able to monitor hundreds of proteins quantitatively in large cohorts patients. However, the fine characteristics some most dominant serum, immunoglobulins, are these studies often ignored, due their vast, highly personalized, diversity sequences. Here, we focus exclusively on personalized features serum proteome distinctively chose study individual samples from a low population: elderly donors infected by severe acute respiratory syndrome corona virus...
There is a growing interest for investigating endogenous peptides from human biofluids which may provide yet unknown functional benefits or an early indication of disease states as potential biomarkers. A major technical bottleneck in the investigation body fluids, e.g., serum, urine, saliva, and milk, that each these fluids seems to require unique workflows peptide extraction analysis. Thus, protocols optimized serum cannot be directly translated milk. One biofluid readily available, but...
Mother's own milk is the first choice for feeding preterm infants, but when not available, pasteurized human donor (PDM) often used. Infants fed PDM have difficulties maintaining appropriate growth velocities. To assess most basic elements of nutrition, we tested hypotheses that fatty acid and amino composition highly variable standard pooling practices attenuate variability; however, total nutrients may be limiting without supplementation due to late lactational stage milk.A prospective...
Human milk oligosaccharides (HMOs) represent the third most abundant fraction of biomolecules in human (HM) and play a crucial role infant health development. The unique contributions HMOs to healthy development breast-fed infants are assumed rely on extraordinary complexity diversity HMO isomeric structures, which turn still cause huge analytical challenge. Many contemporary methods aiming for more detailed characterization combine ion mobility (IM) with LC-MS enhanced structural resolution...
Abstract Background Since the outbreak of COVID-19, many put their hopes in rapid development effective immunizations. For now patient isolation, physical distancing and good hygiene are sole measures for prevention. Processed breast milk with antibodies against SaRS-CoV-2 may serve as additional protection. We aimed to determine presence neutralization capacity breastmilk mothers who have recovered from COVID-19. Methods This prospective case control study included lactating mothers,...
Recently, a mass spectrometry-based approach was introduced to directly assess the IgG1 immunoglobulin clonal repertoires in plasma. Here we expanded upon this by describing technique specifically repertoire of another important class molecules, IgA1, and show it is efficiently robustly applicable either milk or plasma samples. Focusing on two individual healthy donors, whose sampled longitudinally during first 16 weeks lactation, demonstrate that total sIgA1 dominated only 50-500 clones,...
The Alpha-1-Antitrypsin (A1AT) protein is an important protease inhibitor highly abundant in human serum and other body fluids. Additional to functioning as a inhibitor, A1AT acute phase protein. Here, we set out compare the proteoform profiles of purified from milk eight healthy donors determine origin A1AT. Following affinity purification, size-exclusion chromatography coupled native mass spectrometry was used monitor individual comparing inter- intra-donor profiles. were found be...
Maternal supplementation with 1000 mg/day docosahexaenoic acid (DHA) provides third trimester DHA accretion levels in breast milk for the preterm infant. We hypothesized that to mothers providing breastmilk extremely infants would result decreased inflammatory markers, Mother/infant dyads (n = 27) were enrolled at birth and assigned receive 200 or of DHA. Milk plasma samples analyzed fatty acids markers. Decreases inflammation observed both maternal infant correlated red blood cell (RBC)...
Immunoglobulins are the primary protective products in human milk and responsible for transferring maternal pathogen memory to infant, providing protection by binding recognized pathogens inhibiting virulence. To better understand potentially protective/anti-infective compounds milk, establishment of milk-tailored analytical approaches is crucial, as most contemporary methods have been optimized plasma or serum. One prominent immunoglobulins secretory immunoglobulin A (sIgA), which may be...
The fatty acids (FA) of human milk, including DHA, NA, and BCFA may confer distinct neurological immune benefits to the infant. Milk FA profile varies with maternal diet, but global comparative data on milk composition is lacking. To address this gap, we examined concentrations in from three urban populations differing diets: Cincinnati, Ohio, USA; Shanghai, China; Mexico City, Mexico. Sample collection was standardized as part GEHM cohort study. Enrollment limited healthy mothers term,...
Abstract Here, by using mass spectrometry-based methods IgG1 and IgA1 clonal repertoires were monitored quantitatively longitudinally in more than 50 individual serum samples obtained from 17 COVID-19 patients admitted to intensive care units because of acute respiratory distress syndrome. These serological profiles used examine how each patient reacted a severe SARS-CoV-2 infection. All donors revealed unique polyclonal changes after Substantial over time the and/or observed patients, with...
Background: Poor diet quality and high body mass index (BMI) contribute to inflammation, which may influence human milk composition exclusive breastfeeding (EBF) duration. Objective: We evaluated maternal prepregnancy BMI as predictors of C-reactive protein (CRP) long chain fatty acid concentrations (%LCFA), EBF Methods: utilized the Global Exploration Human Milk Study-Cincinnati subset (n=114), where healthy dyads continued follow-up if ≥75% feeds were breastmilk at 4 weeks postpartum....
Introduction Upon vaccination against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) humans will start to produce antibodies targeting virus specific antigens that end up in circulation. In lactating women such also breastmilk, primarily the form of secretory immunoglobulin A1 (SIgA1), most abundant (Ig) human milk. Here we set out investigate SIgA1 clonal repertoire response repeated SARS-CoV-2 vaccination, using a LC–MS fragment antigen-binding (Fab) profiling approach....
Abstract The most abundant immunoglobulin present in the human body is IgA1. It has highest concentrations at mucosal lining and biofluids such as milk second class of antibodies serum. We assessed structural diversity clonal repertoire IgA1-containing molecular assemblies longitudinally serum from three donors using a mass spectrometry-based approach. IgA-containing molecules purified or were by release subsequent analysis their Fab fragments. Our data revealed that IgA1 consists two...