A5052857408- Nitric Oxide and Endothelin Effects
- Redox biology and oxidative stress
- Pituitary Gland Disorders and Treatments
- Sulfur Compounds in Biology
- Estrogen and related hormone effects
- Neuroendocrine Tumor Research Advances
- Cancer, Lipids, and Metabolism
- Cancer Cells and Metastasis
- Advanced Proteomics Techniques and Applications
- Eicosanoids and Hypertension Pharmacology
- Renin-Angiotensin System Studies
- Acute Myeloid Leukemia Research
- Cancer, Hypoxia, and Metabolism
- Advanced Chemical Sensor Technologies
- Colorectal Cancer Treatments and Studies
- Cancer Mechanisms and Therapy
- Liver physiology and pathology
- Hormonal Regulation and Hypertension
- Metabolomics and Mass Spectrometry Studies
- Mesenchymal stem cell research
- Angiogenesis and VEGF in Cancer
- Cancer-related Molecular Pathways
- Ferroptosis and cancer prognosis
- Cancer Research and Treatments
- Cassava research and cyanide
Instituto Maimónides de Investigación Biomédica de Córdoba
2013-2024
University of Córdoba
2012-2024
Hospital Universitario Reina Sofía
2006-2024
Spanish Biomedical Research Centre in Physiopathology of Obesity and Nutrition
2024
Centro de Investigación Biomédica en Red de Cáncer
2017-2020
Centro de Investigación Biomédica en Red
2014-2017
Instituto de Salud Carlos III
2014-2016
Hospital General Universitario Gregorio Marañón
2013
Universidad Carlos III de Madrid
2013
Charité - Universitätsmedizin Berlin
2012
The induction of polyploidy is considered the reproductive end cells, but there evidence that polyploid giant cancer cells (PGCCs) contribute to cell repopulation during tumor relapse. However, role these in development, progression and response therapy colon remains undefined. Therefore, main objective this study was investigate generation PGCCs identify mechanisms formation. Treatment HCT-116 Caco-2 with hypoxia mimic CoCl2 induced formation larger nuclear size (PGCCs), while normal...
Desmopressin is a synthetic agonist of vasopressin receptors (AVPRs). The desmopressin stimulation test used in the diagnosis and postsurgery prognosis Cushing disease (CD). However, cellular molecular mechanisms underlying desmopressin-induced ACTH increase patients with CD are poorly understood.The objectives this study were to determine, for first time, whether acts directly exclusively on pituitary corticotropinoma cells stimulate expression/release elucidate involved CD.A total 8 normal...
We explored whether the proteomic analysis of exhaled breath condensate (EBC) may provide biomarkers for noninvasive screening early detection lung cancer (LC). EBC was collected from 192 individuals [49 control (C), 49 risk factor-smoking (S), 46 chronic obstructive pulmonary disease (COPD) and 48 LC]. With use liquid chromatography tandem mass spectrometry, 348 different proteins with a pattern among four groups were identified in samples. Significantly more LC compared other (C: 12.4 ±...
Despite the demonstrated benefits of anti-EGFR/VEGF targeted therapies in metastatic colorectal cancer (mCRC), many patients initially respond, but then show evidence disease progression. New therapeutic strategies are needed to make action available drugs more efficient. Our study aimed explore whether simultaneous targeting EGFR/VEGF and cyclooxygenase-2 (COX-2) may aid treatment management mCRC patients. The dual tyrosine kinase inhibitor AEE788 celecoxib were used inhibit EGFR/VEGFR...
// Raúl M. Luque 1, 2, 3, 4, 5, *, # , Laura López-Sánchez * Alicia Villa-Osaba Isabel 3 Ana L. Santos-Romero 6 Elena Yubero-Serrano 7 María Cara-García Marina Álvarez-Benito José López-Miranda Manuel D. Gahete and Justo P. Castaño 1 Maimonides Institute of Biomedical Research Cordoba (IMIBIC), Córdoba, Spain 2 Department Cell Biology, Physiology Immunology, University Hospital Universitario Reina Sofia, 4 CIBERobn, 5 ceiA3, Mammary Gland Unit, Sofía, Lipids Atherosclerosis These authors...
Nitric oxide (NO) has been highlighted as an important agent in cancer-related events. Although the inducible nitric synthase (iNOS) isoform received most attention, recent studies literature indicate that endothelial isoenzyme (eNOS) can also modulate different tumor processes including resistance, angiogenesis, invasion, and metastasis. However, role of eNOS cancer stem cell (CSC) biology mesenchymal tumors is unknown. Here, we show was significantly upregulated VilCre ERT2 Apc fl/+ fl/fl...
Somatostatin receptors (ssts) are expressed in thyroid cancer cells, but their biological significance is not well understood. The aim of this study was to assess ssts differentiated (WDTC) and poorly (PDTC) by means imaging molecular tools its relationship with the efficacy somatostatin analog treatment. Thirty-nine cases carcinoma were evaluated (20 PDTC 19 WDTC). Depreotide scintigraphy mRNA levels sst-subtypes, including truncated variant sst5TMD4, carried out. scans positive recurrent...
Abstract Chimeric somatostatin/dopamine compounds such as BIM-23A760, an sst2/sst5/D 2 receptors-agonist, have emerged promising new approaches to treat pituitary adenomas. However, information on direct in vitro effects of BIM-23A760 normal and tumoral pituitaries remains incomplete. The objective this study was analyze functional parameters (Ca 2+ signaling, hormone expression/secretion, cell viability apoptosis) adenomas (n = 74), compare with the responses primate human 3–5). Primate...
This study represents a novel proof of concept the clinical utility miRNAs from exhaled breath condensate (EBC) as biomarkers lung cancer (LC). Genome-wide miRNA profiling and machine learning analysis were performed on EBC 21 healthy volunteers LC patients. The levels 12 significantly altered in patients where specific signature miR-4507, miR-6777-5p miR-451a distinguished these with high accuracy. Besides, distinctive profile between adenocarcinoma squamous cell carcinoma was observed,...
The liver is one organ clearly influenced by nitric oxide (NO), and acute chronic exposure to this substance has been associated with distinct patterns of disease. Disruption or deregulation S-nitrosothiol (SNO) signalling leads impairment cellular function disease, study was aimed identify potential targets for protein S-nitrosation during alteration SNO homeostasis in human hepatocytes. Cells were treated S-nitroso-L-cysteine (CSNO), an effective physiological nitrosothiol delivering NO...
Abstract Introduction Protein denitrosylation by thioredoxin reductase (TrxR) is key for maintaining S -nitrosothiol (SNO) homeostasis, although its role in tumor progression unknown. Therefore, the present study aimed to assess of altered SNO homeostasis breast cancer cells. Methods The impairment cells was achieved with highly specific TrxR inhibitor auranofin and/or exposure -nitroso- L -cysteine. -nitrosylated proteins were detected using biotin switch assay. Estrogen receptor (ER) alpha...
Currently, there are no predictive biomarkers for anti-angiogenic strategies in cancer, but response to drugs is associated with development of hypertension secondary treatment. Therefore, this study explored the clinical relevance genetic polymorphisms some components renin-angiotensin system (RAS).Genomic DNA was isolated from peripheral blood 95 metastatic breast or colorectal cancer patients treated bevacizumab, and AGTR1-A1166C (rs5186), AGT-M235T (rs699) SNPs ACE I/D (rs4646994) were...
// Araceli Valverde 1 , Jon Peñarando 1, 2 Amanda Cañas Laura M. López-Sánchez Francisco Conde Silvia Guil-Luna Vanessa Hernández Carlos Villar 3 Cristina Morales-Estévez 4 Juan de la Haba-Rodríguez 2, Enrique Aranda Antonio Rodríguez-Ariza Instituto Maimónides Investigación Biomédica Córdoba (IMIBIC), Córdoba, Spain Centro Investigacion en Red Cáncer, Madrid, Unidad Gestión Clínica Anatomía Patológica, Hospital Universitario Reina Sofía, Oncología, Correspondence to: Rodríguez-Ariza, email:...
Abstract Newly emerged proteomic methodologies, particularly data‐independent acquisition (DIA) analysis–related approaches, would improve current gene expression–based classifications of colorectal cancer (CRC). Therefore, this study was aimed to identify protein expression signatures using SWATH‐MS DIA and targeted data extraction, aid in the classification molecular subtypes CRC advance diagnosis development new drugs. For purpose, 40 human samples 7 healthy tissue were subjected...
Tumor budding has been found to be of prognostic significance for several cancers, including colorectal cancer (CRC). Additionally, the molecular classification CRC led identification different immune microenvironments linked distinct prognosis and therapeutic response. However, association between tumor subtypes profiles have not fully elucidated. This study focused, firstly, on validation derived xenograft models (PDXs) evaluation their human counterparts and, secondly, microenvironment by...
Hepatic injury by d-galactosamine (d-GalN) is a suitable experimental model of hepatocellular injury. The induction oxidative and nitrosative stress participates during d-GalN-induced cell death in cultured rat hepatocytes. This study aimed to identify protein expression changes the apoptosis necrosis d-GalN human hepatocytes.A proteomic approach was used proteins involved those altered tyrosine nitration. A high dose (40 mM) induce primary culture Cellular lysates prepared at different...