A5084779983- Ion Transport and Channel Regulation
- Ion channel regulation and function
- Hormonal Regulation and Hypertension
- Neuroscience and Neuropharmacology Research
- Healthcare and Venom Research
- ATP Synthase and ATPases Research
- Venomous Animal Envenomation and Studies
- Drug Transport and Resistance Mechanisms
- Renin-Angiotensin System Studies
- Insect and Pesticide Research
- Science and Education Research
- Complementary and Alternative Medicine Studies
- Parasites and Host Interactions
- Ion Channels and Receptors
- Bee Products Chemical Analysis
- Magnesium in Health and Disease
- Physiological and biochemical adaptations
- NF-κB Signaling Pathways
- Trypanosoma species research and implications
- Pharmacogenetics and Drug Metabolism
- Nitric Oxide and Endothelin Effects
- Inflammatory mediators and NSAID effects
- Medicinal Plant Extracts Effects
- Phytochemistry and Bioactive Compounds
- Analytical Methods in Pharmaceuticals
Universidade Federal do Rio de Janeiro
2015-2025
Universidade Estadual Paulista (Unesp)
2020
Instituto Vital Brazil (Brazil)
2020
Universidad de Morón
2010
University of Toledo
2010
Universidade do Estado do Rio de Janeiro
1994
Here, we show that the Na/K-ATPase interacts with caveolin-1 (Cav1) and regulates Cav1 trafficking. Graded knockdown of decreases plasma membrane pool Cav1, which results in a significant reduction number caveolae on cell surface. These effects are independent pumping function Na/K-ATPase, instead depend interaction between mediated by an N-terminal caveolin-binding motif within ATPase α1 subunit. Moreover, increases basal levels active Src stimulates endocytosis from membrane....
Parotoid gland secretions of toad species are a vast reservoir bioactive molecules with wide range biological properties. Herein, for the first time, it is described isolation by preparative reversed-phase HPLC and structure elucidation NMR spectroscopy and/or mass spectrometry nine major bufadienolides from parotoid Cuban endemic Peltophryne fustiger: ψ-bufarenogin, gamabufotalin, bufarenogin, arenobufagin, 3-(N-suberoylargininyl) marinobufagin, bufotalinin, telocinobufagin, marinobufagin...
Cardiotonic steroids are used to treat heart failure and arrhythmia have promising anticancer effects. The prototypic cardiotonic steroid ouabain may also be a hormone that modulates epithelial cell adhesion. consist of nucleus lactone ring, their biological effects depend on the binding receptor, Na,K-ATPase, through which, they inhibit Na+ K+ ion transport activate several intracellular signaling pathways. In this study, we added styrene group ring digoxin, obtain 21-benzylidene digoxin...
Cardiac glycosides have been extensively used in the treatment of congestive heart failure for more than 200 years. Recently, cardenolides and bufadienolides were isolated from mammalian tissue are considered as a new class steroidal hormones. The aim present work was to characterize interaction between most clinical cardiac glycoside digoxin known exist endogenously, i.e., ouabain, marinobufagin telocinobufagin, on human kidney Na+/K+-ATPase. Inhibition Na+/K+-ATPase activity crude membrane...
Bufadienolides are structurally related to the clinically relevant cardenolides (e.g., digoxin) and now considered as endogenous steroid hormones. Binding of ouabain Na(+)-K(+)-ATPase has been associated, in kidney cells, activation Src kinase pathway internalization. Nevertheless, whether this cascade also occurs with other cardiotonic steroids leads diuresis natriuresis isolated intact is still unknown. In present work, we perfused rat kidneys for 120 min bufalin (1, 3, or 10 μM) measured...
Background and Aims Schistosomiasis is an intravascular parasitic disease associated with inflammation. Endothelial cells control leukocyte transmigration vascular permeability being modulated by pro-inflammatory mediators. Recent data have shown that endothelial primed in vivo the course of a keep information culture. Herein, we evaluated impact schistosomiasis on cell-regulated events vitro. Methodology Principal Findings The experimental groups consisted Schistosoma mansoni-infected...
Novel roles for the interaction of cardiotonic steroids to Na + /K -ATPase have been established in recent years. The aim this study was investigate intracellular signaling events downstream action ouabain on Sertoli cell obtained from immature rats. Treatment cells with (1 μM) induced a rapid and transient increase extracellular signal-regulated kinase (ERK1/2 or MAPK3/1) phosphatidylinositol 3-kinase (PI3K)/serine–threonine protein (AKT) phosphorylation. Also, upregulated expression cyclin...
Cardiotonic steroids, such as ouabain and digoxin, are known to bind Na + /K -ATPase promote several biological activities, including anti-inflammatory activity. However, there still no reports in the literature about inflammation marinobufagenin, a cardiotonic steroid from bufadienolide family endogenously found mammals. Therefore, aim of this work was analyze, vivo vitro , role marinobufagenin acute inflammation. Swiss mice were treated with 0.56 mg/kg intraperitoneally (i.p.) zymosan (2...
Cardiotonic steroids (CS), natural compounds with traditional use in cardiology, have been recently suggested to exert potent anticancer effects. However, the repertoire of molecules Na,K-ATPase activity and properties is limited. This paper describes synthesis 6 new digoxin derivatives substituted (on C17-butenolide) γ-benzylidene group their cytotoxic effect on human fibroblast (WI-26 VA4) cancer (HeLa RKO) cell lines as well expression. As digoxin, compound BD-4 was almost 100-fold more...
LASSBio-1135 is an imidazo[1,2-a]pyridine derivative with high efficacy in screening models of nociception and inflammation, presumed as a weak COX-2 inhibitor. In order to tease out its mechanism action, we investigated others possible target for LASSBio-1135, such TNF-α TRPV1, better characterize it multitarget compound useful the treatment chronic pain. TRPV1 modulation was assessed TRPV1-expressing Xenopus oocytes against capsaicin low pH-induced current. Modulation production evaluated...
Bufadienolides are cardiotonic steroids (CTS) identified in mammals. Besides Na⁺/K⁺-ATPase inhibition, they activate signal transduction via protein⁻protein interactions. Diversity of endogenous bufadienolides and mechanisms action may indicate the presence functional selectivity unique cellular outcomes. We evaluated whether telocinobufagin marinobufagin induce changes proliferation or viability pig kidney (LLC-PK1) cells involved these changes. In some experiments, ouabain was used as a...
Diene valepotriates obtained from <i>Valeriana glechomifolia</i> present antidepressant-like activity, mediated by dopaminergic and noradrenergic neurotransmissions. Also, previous studies have shown inhibitory activity of diene towards Na<sup>+</sup>/K<sup>+</sup>-ATPase the rat brain <i>in vitro</i>. Nevertheless, vivo</i> regarding action on this enzyme are still lacking. Considering that cerebral is involved in depressive disorders, aim study was to investigate effects acute (5 mg/kg, p....
Massive, Africanized honeybee attacks have increased in Brazil over the years. Humans and animals present local systemic effects after envenomation, there is no specific treatment for this potentially lethal event. This study evaluated ability of a new Apilic antivenom, which composed F(ab')2 fraction immunoglobulins heterologous hyperimmune equine serum, to neutralize A. mellifera venom melittin, vitro vivo, mice. Animal experiments were performed according with ethics committee license...
Valepotriates are iridoids found in variable amounts Valerianaceae and might be among the bioactive compounds which confer anxiolytic properties to Valeriana species. On other hand, unspecific cytotoxicity has also been described. Presently, however, no particular molecular target defined for these compounds. Here we studied effect of valtrate, acevaltrate, 1- β-acevaltrate isolated from glechomifolia on enzymatic activity rat P-type ATPases. did not affect skeletal muscle sarco/endoplasmic...