A5002247187- Cancer Cells and Metastasis
- Cancer-related molecular mechanisms research
- Genetic and phenotypic traits in livestock
- Single-cell and spatial transcriptomics
- Cancer Genomics and Diagnostics
- Animal Diversity and Health Studies
- RNA modifications and cancer
- Molecular Biology Techniques and Applications
- Cancer-related Molecular Pathways
- Bat Biology and Ecology Studies
- Hair Growth and Disorders
- Developmental Biology and Gene Regulation
- Biochemical Analysis and Sensing Techniques
- Cancer and Skin Lesions
- BRCA gene mutations in cancer
- Digestive system and related health
- Species Distribution and Climate Change
- Genetic diversity and population structure
- Wildlife Ecology and Conservation
- Wnt/β-catenin signaling in development and cancer
- melanin and skin pigmentation
- Fibroblast Growth Factor Research
- Metastasis and carcinoma case studies
- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
Walter and Eliza Hall Institute of Medical Research
2017-2025
The University of Melbourne
2009-2019
The mammary epithelium comprises two primary cellular lineages, but the degree of heterogeneity within these compartments and their lineage relationships during development remain an open question. Here we report single-cell RNA profiling mouse epithelial cells spanning four developmental stages in post-natal gland. Notably, undergoes a large-scale shift gene expression from relatively homogeneous basal-like program pre-puberty to distinct lineage-restricted programs puberty. Interrogation...
Abstract Fibroblasts form a major component of the stroma in normal mammary tissue and breast tumors. Here, we have applied longitudinal single-cell transcriptome profiling >45,000 fibroblasts mouse gland across five different developmental stages during oncogenesis. In gland, diverse stromal populations were resolved, including lobular-like fibroblasts, committed preadipocytes adipogenesis-regulatory, as well cycling puberty pregnancy. These specialized cell types appear to emerge from...
Abstract Background Heterogeneity within the mouse mammary epithelium and potential lineage relationships have been recently explored by single-cell RNA profiling. To further understand how cellular diversity changes during ontogeny, we profiled single cells from nine different developmental stages spanning late embryogenesis, early postnatal, prepuberty, adult, mid-pregnancy, late-pregnancy, post-involution, as well transcriptomes of micro-dissected terminal end buds (TEBs) subtending ducts...
Abstract Hair follicles cycle through expansion, regression and quiescence. To investigate the role of MCL‑1, a BCL‑2 family protein with anti‑apoptotic apoptosis‑unrelated functions, we delete Mcl‑1 within skin epithelium using constitutive inducible systems. Constitutive deletion does not impair hair follicle organogenesis but leads to gradual loss elimination stem cells. Acute rapidly depletes activated cells completely blocks depilation‑induced regeneration in adult mice, while quiescent...
The development of high-quality chromosomally assigned reference genomes constitutes a key feature for understanding genome architecture species and is critical the discovery genetic blueprints traits biological significance. South American camelids serve people in extreme environments are important fiber companion animals worldwide. Despite this, alpaca lags far behind those available other domestic species. Here we produced chromosome-level improved assembly using DNA same female Huacaya...
White-spotting patterns in mammals can be caused by mutations the gene KIT, whose protein is necessary for normal migration and survival of melanocytes from neural crest. The alpaca (Vicugna pacos) blue-eyed white (BEW) phenotype characterized 2 blue eyes a solid coat over whole body. Breeders hypothesize that BEW alpacas combination causing gray fleece white-spotting gene. We performed an association study using KIT flanking intragenic markers with 40 unrelated alpacas, which 17 were BEW....
Information based on the accurate identification of species is a vital component for achieving successful outcomes biodiversity conservation and management. It difficult to manage that are poorly known or misidentified with other similar species. This particularly problematic rare threatened Species listed under endangered classification schemes need be identified accurately categorised correctly so efforts appropriately allocated. In Australia, emballonurid Saccolaimus saccolaimus currently...
The transcription factor EHF is highly expressed in the lactating mammary gland, but its role development and tumorigenesis not fully understood. Utilizing a mouse model of Ehf deletion, herein, we demonstrate that loss impairs lobuloalveolar differentiation at late pregnancy, indicated by significantly reduced levels milk genes lipids, fewer differentiated alveolar cells, an accumulation progenitor cells. Further, deletion increased proliferative capacity attenuated prolactin-induced...