A5012414728- MicroRNA in disease regulation
- Bioinformatics and Genomic Networks
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- Prostate Cancer Treatment and Research
- Gene expression and cancer classification
- Gene Regulatory Network Analysis
- Prostate Cancer Diagnosis and Treatment
- Single-cell and spatial transcriptomics
- Functional Brain Connectivity Studies
- Breast Cancer Treatment Studies
- CRISPR and Genetic Engineering
- Neural Networks and Applications
- RNA and protein synthesis mechanisms
- Fish Biology and Ecology Studies
- Leptospirosis research and findings
- Computational Drug Discovery Methods
- Epigenetics and DNA Methylation
- Wnt/β-catenin signaling in development and cancer
- COVID-19 Clinical Research Studies
- Immune responses and vaccinations
- Breast Lesions and Carcinomas
- Genetic Neurodegenerative Diseases
- Psychedelics and Drug Studies
- Cancer Diagnosis and Treatment
University of Turin
2017-2024
Human Technopole
2022-2024
Babraham Institute
2017-2021
Scuola Normale Superiore
2014-2018
University of Pisa
1991-2014
Hospital of Prato
2014
Ospedale di Parma
1991-2005
New York University
1988
Summary The brains of teleost fish show extensive adult neurogenesis and neuronal regeneration. patterns gene regulation during brain aging are unknown. short‐lived Nothobranchius furzeri shows markers including reduced learning performances, gliosis, neurogenesis. We used RNA ‐seq to quantify genome‐wide transcript sampled five different time points characterize whole‐genome N. . Comparison with human datasets revealed conserved up‐regulation ribosome, lysosome, complement activation...
A widespread modulation of gene expression occurs in the aging brain, but little is known as to upstream drivers these changes. MicroRNAs emerged fine regulators many biological contexts and they are modulated by age. may therefore be part global correlated with aging-related phenotypes.Here, we show that microRNA-29 (miR-29) induced during short-lived turquoise killifish brain genetic antagonism its function induces a gene-expression signature typical aging. Mechanicistically, identified...
Abstract Inflammation is a common condition of prostate tissue, whose impact on carcinogenesis highly debated. Microbial colonization well-documented cause small percentage prostatitis cases, but it remains unclear what underlies the majority sterile inflammation reported. Here, androgen- independent fluctuations PSA expression in cells have lead us to identify prominent function Transient Receptor Potential Cation Channel Subfamily M Member 8 (TRPM8) gene inflammation. Prostate secret TRPM8...
Cancer stem cells (CSCs) are the main drivers of disease progression and chemotherapy resistance in breast cancer. Tumor chemoresistance might then be prevented by CSC-targeted therapies. We previously demonstrated that Toll-like Receptor (TLR)2 is overexpressed CSCs fuels their self-renewal. Here, we show high TLR2 expression linked to poor prognosis cancer patients, therefore representing a candidate target for treatment. By using novel mammary cancer-prone TLR2KO mouse model, demonstrate...
Abstract Background Tumor progression is based on a close interaction between cancer cells and MicroEnvironment (TME). Here, we focus the role that Cancer Associated Fibroblasts (CAFs), Mesenchymal Stem Cells (MSCs) microRNAs (miRs) play in breast melanoma malignancy. Methods We used public databases to investigate miR-214 expression stroma compartment of primary human samples evaluated tumor formation dissemination following cell injections overexpressing (miR-214 over ) knock out ko mice....
The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has fueled the COVID-19 pandemic with its enduring medical and socioeconomic challenges because subsequent waves long-term consequences great concern. Here, we chart molecular basis pathogenesis by analyzing patients' immune responses at single-cell resolution across disease course severity. This approach confirms cell subpopulation-specific dysregulation in severity identifies a severity-associated activation...
The main drawback of BRAF/MEK inhibitors (BRAF/MEKi)-based targeted therapy in the management BRAF-mutated cutaneous metastatic melanoma (MM) is development therapeutic resistance. We aimed to assess this context role mTORC2, a signaling complex defined by presence essential RICTOR subunit, regarded as an oncogenic driver several tumor types, including MM.
Breast cancer is a heterogeneous disease whose clinical management very challenging. Although specific molecular features characterize breast subtypes with different prognosis, the identification of markers predicting outcome within single still lags behind. Both non-canonical Wingless-type MMTV Integration site (WNT) and Signal Transducer Activator Transcription (STAT)3 pathways are often constitutively activated in tumors, both can induce small GTPase Ras Homolog Family Member U RhoU. Here...
Abstract Background The short-lived fish Nothobranchius furzeri is the shortest-lived vertebrate that can be cultured in captivity and was recently established as a model organism for aging research. Small non-coding RNAs, especially miRNAs, are implicated age dependent control of gene expression. Results Here, we present comprehensive catalogue miRNAs several other RNA classes (ncRNAs) . Analyzing multiple small RNA-Seq libraries, show most these identified expressed at least one seven...
In the last decade, our group has intensively studied annual fish Nothobranchius furzeri as a new experimental model in Biology specifically applied to aging research. We previously adult neuronal stem cells of N. vivo and we demonstrated an age-dependent decay neurogenesis. More recently identified quantified expression miRNAs brain detected 165 conserved found that this is associated with coherent up-regulation well-known tumor suppressor miRNAs, well down-regulation onco miRNAs~- present...
Abstract Psychedelic drugs are gaining attention from the scientific community as potential new compounds for treatment of psychiatric diseases such mood and substance use disorders. The 5‐HT 2A receptor has been identified main molecular target, early studies pointed to an effect on expression neuroplasticity genes. Analysing RNA‐seq data prefrontal cortex rats chronically treated with lysergic acid diethylamide (LSD), we describe psychedelic‐induced rewiring gene co‐expression networks,...
21q22.2–3 deletion is the most common copy number alteration in prostate cancer (PCa). The genomic rearrangement results androgen-dependent de novo expression of ETS-related gene (ERG) cells, a condition promoting tumor progression to advanced stages disease. Interestingly, ERG characterizes 5–30% precursor lesions – High Grade Prostatic Intraepithelial Neoplasia (HGPIN) - where its role remains unclear. Here, by combining organoids technology with Click-chemistry coupled Mass Spectrometry,...
Abstract p140Cap is an adaptor protein involved in assembling multi-protein complexes regulating several cellular processes. acts as a tumor suppressor breast cancer (BC) and neuroblastoma patients, where its expression correlates with better prognosis. The role of metabolism remains largely unknown. Here we study the modulation mevalonate (MVA) pathway BC cells. MVA responsible for biosynthesis cholesterol non-sterol isoprenoids often deregulated cancer. We found that both vitro vivo, cells...
Transcriptome data provide a valuable resource for the study of cancer molecular mechanisms, but technical biases, sample heterogeneity, and small sizes result in poorly reproducible lists regulated genes. Additionally, presence multiple cellular components contributing to development complicates interpretation bulk transcriptomic profiles. To address these issues, we collected 48 microarray datasets derived from laser capture microdissected stroma or epithelium breast tumors performed...
The systematic identification of tumour vulnerabilities through perturbational experiments on cancer models, including genome editing and drug screens, is playing a crucial role in combating cancer. This collective effort known as the Cancer Dependency Map (DepMap). 1st European Symposium (EuroDepMap), held Milan last May, featured talks, roundtable discussion, poster session, showcasing latest discoveries future challenges related to DepMap. symposium aimed facilitate interactions among...
Abstract Background Biological networks are often used to describe the relationships between relevant entities, particularly genes and proteins, a powerful tool for functional genomics. Many important biological problems can be investigated by comparing different conditions or obtained with techniques. Findings We show that contrast subgraphs, recently introduced technique identify most structural differences 2 networks, provide versatile gene protein of diverse origin. demonstrate use...