A5111972593- Advanced biosensing and bioanalysis techniques
- Aquatic Ecosystems and Phytoplankton Dynamics
- Photosynthetic Processes and Mechanisms
- Metalloenzymes and iron-sulfur proteins
- Metal-Catalyzed Oxygenation Mechanisms
- Marine and coastal ecosystems
- DNA and Nucleic Acid Chemistry
- Protein Hydrolysis and Bioactive Peptides
- Advanced Photocatalysis Techniques
- Nanoplatforms for cancer theranostics
- RNA and protein synthesis mechanisms
- Epigenetics and DNA Methylation
- Luminescence and Fluorescent Materials
- Peptidase Inhibition and Analysis
- Photoreceptor and optogenetics research
- Soil Moisture and Remote Sensing
- Biosensors and Analytical Detection
- Corrosion Behavior and Inhibition
- Hemoglobin structure and function
- Electrochemical Analysis and Applications
- DNA Repair Mechanisms
- RNA Interference and Gene Delivery
- Porphyrin Metabolism and Disorders
- Electrocatalysts for Energy Conversion
- Nanocluster Synthesis and Applications
Chinese Academy of Sciences
2015-2025
Shandong University
2022-2025
Changchun Institute of Applied Chemistry
2020-2025
University of Science and Technology of China
2020-2025
Shanghai Advanced Research Institute
2021-2025
Chinese University of Hong Kong
2025
University of Hong Kong
2025
Institute of Special Animal and Plant Sciences
2025
Chinese Academy of Agricultural Sciences
2025
Beijing Institute of Technology
2015-2024
Genetic drivers of cancer can be dysregulated through epigenetic modifications DNA. Although the critical role DNA 5-methylcytosine (5mC) in regulation transcription is recognized, functions other non-canonical remain obscure. Here, we report identification novel N6-methyladenine (N6-mA) human tissues and implicate this mark disease, specifically highly malignant brain glioblastoma. Glioblastoma markedly upregulated N6-mA levels, which co-localized with heterochromatic histone modifications,...
Here, we report a β-galactosidase (β-Gal)-responsive photochromic fluorescent probe, NpG, that was designed to prebind human serum albumin (HSA) form the probe/protein hybrid, NpG@HSA. The formation of NpG@HSA led an increase in fluorescence emission (520 nm) corresponding binding naphthalimide unit with HSA. In addition, this enabled visualization spiropyran aqueous media. Our hybrid approach afforded unique imaging platform enhanced cell permeability and solubility capable visualizing...
Abstract While the unique physicochemical properties of nanomaterials that enable regulation nanozyme activities are demonstrated in many systems, quantitative relationships between structure and their enzymatic remain poorly understood, due to heterogeneity compositions active sites these nanomaterials. Here, inspired by metalloenzymes with well‐defined metal–ligand coordination, a set substituted metal–organic frameworks (MOFs) similar coordination is employed investigate relationship...
Top model: Heme–copper oxidase (HCO) contains a histidine–tyrosine cross-link in its heme a3/CuB oxygen reduction center. A functional model of HCO was obtained through the genetic incorporation unnatural amino acid imiTyr, which mimics Tyr–His cross-link, and CuB site into myoglobin (see picture). Like HCO, this small soluble protein exhibits selective O2-reduction activity while generating little reactive species. Detailed facts importance to specialist readers are published as "Supporting...
Significance Nature spent millions of years to evolve electron transfer proteins that span a wide range reduction potentials (E°′) under physiological conditions, from −1 V 1 vs. standard hydrogen electrode. Understanding the rules govern such tuning within similar classes metalloproteins enables scientists rationally tune E°′of their catalysts without changing active site. An ultimate test understanding is achieve entire E°′ single protein, feat has not been achieved yet. Herein, we...
There are 20 proteinogenic amino acids and a limited number of cofactors naturally available to build enzymes. Genetic codon expansion enables us incorporate more than 200 unnatural into proteins using cell translation machinery, greatly expanding structures protein chemists. Such tools enable scientists mimic the active site an enzyme tune enzymatic activity, anchor cofactors, immobilize enzymes on electrode surfaces. Non-native can be incorporated through covalent or noncovalent...
NAFLD is a key component of metabolic syndrome, ranging from nonalcoholic fatty liver to NASH, and now becoming the leading cause cirrhosis HCC worldwide. However, due complex unclear pathophysiological mechanism, there are no specific approved agents for treating NASH. Breviscapine, natural flavonoid prescription drug isolated traditional Chinese herb Erigeron breviscapus, exhibits wide range pharmacological properties, including effects on metabolism. anti-NASH efficacy mechanisms...
HP1 proteins are thought to be modulators of chromatin organization in all mammals, yet their exact physiological function remains unknown. In a first attempt elucidate the these vivo, we disrupted murine Cbx1 gene, which encodes HP1-β isotype, and show that Cbx1−/−-null mutation leads perinatal lethality. The newborn mice succumbed acute respiratory failure, whose likely cause is defective development neuromuscular junctions within endplate diaphragm. We also observe aberrant cerebral...
Terminal oxidases catalyze four-electron reduction of oxygen to water, and the energy harvested is utilized drive synthesis adenosine triphosphate. While much effort has been made design a catalyst mimicking function terminal oxidases, most biomimetic catalysts have lower activity than native oxidases. Herein we report designed oxidase in myoglobin with an O2 rate (52 s(-1)) comparable that cytochrome (cyt) cbb3 (50 under identical conditions. We achieved this goal by engineering more...
While a conserved tyrosine (Tyr) is found in oxidases, the roles of phenol ring pKa and reduction potential O2 have not been defined despite many years research on numerous oxidases their models. These issues represent major challenges our understanding mechanism bioenergetics. Through genetic incorporation unnatural amino acid analogs Tyr, with progressively decreasing increasing potential, active site functional model oxidase myoglobin, linear dependence both activity fraction H2O...
Due to recent increases in the price of Ni, steel use is currently undergoing a global shift from austenitic stainless steels ferritic steels. In this study, corrosion behavior four types with different Cr contents was investigated study effect content on resistance sulfuric acid solution. The polarization curves highest indicated best resistance. No observed for 24 mass% after potential sweep based ex-situ SEM images. Corrosion resistivity improved high (>24 mass%) because it considered...
The hallmark reaction of P450 monooxygenase involves activation C–H bond and the production a hydroxyl. P450s tailoring natural product could further oxidize hydroxyl to carboxylic acid. However, heterogeneously expressed plant display poor chemo- regioselectivity in microbes, restricting efficient biosynthesis related products. CYP72A63 is enzyme previously used for glycyrrhetinic acid, its selectivity resulted oxidation 11-oxo-β-amyrin mixture rare licorice triterpenoids (glycyrrhetol,...
The development of isoform-specific probe(s) for a target enzyme with multiple homologs is always challenging. Herein, practical strategy was used to design and develop an probe CYP1A1, key cytochrome P450 isoenzyme involved in xenobiotic metabolism bioactivation. On the basis subtle differences 3D structure substrate preference between CYP1A1 its homolog CYP1A2, we proposed that it possible CYP1A1-specific via local modification reaction site on known CYP1A substrates. To validate this...