Alzheimer's disease (AD), the most common neurodegenerative disease, remains unclear in terms of its underlying causative genes and effective therapeutic approaches. Meanwhile, abnormalities iron metabolism have been demonstrated patients mouse models with AD. Therefore, this study sought to find hub based on that can influence diagnosis treatment First, gene expression profiles were downloaded from GEO database, including non-demented (ND) controls AD samples. Fourteen metabolism-related...

Ferroptosis is associated with the prognosis and therapeutic responses of patients various cancers. LncRNAs are reported to exhibit antitumor or oncogenic functions. Currently, few studies have assessed combined effects ferroptosis lncRNAs on therapy stomach cancer. In this study, transcriptomic clinical data were downloaded from TCGA database, ferroptosis-related genes obtained FerrDb database. Through correlation analysis, Cox Lasso algorithm, 10 prognostic (AC009299.2, AC012020.1,...

Lactylation, a newly identified post-translational modification, is uncertain in its implication triple-negative breast cancer (TNBC). In this study, we analyzed 60 TNBC samples using immunohistochemical staining and revealed elevated levels of pan-lactylated proteins specific histone H4K12 lactylation tumor tissues, correlating with progression. Lactate exposure cell lines significantly induced lysine at the site, leading to alterations gene profiles reduced apoptosis. These effects were...

Human Schlafen 5 (SLFN5) has been reported to inhibit or promote cell invasion in tumours depending on their origin. However, its role breast cancer (BRCA) is undetermined.Differential expression analyses using The Cancer Genome Atlas (TCGA) data, clinical samples and lines were performed. Lentiviral knockdown overexpression experiments performed detect changes morphology, molecular markers invasion. Chromatin immunoprecipitation-sequencing (ChIP-Seq) luciferase reporter assays the...

Epithelial-mesenchymal transition (EMT) is critical for carcinoma invasiveness and metastasis. To investigate the role of membrane-type-2 matrix metalloproteinase (MT2-MMP) in EMT, we generated lentiviral constructs wild-type (WT) an inactive Glu260Ala (E260A) mutant MT2-MMP derived stably transfected HCT116 A549 cell lines. WT-transfected cells appeared mesenchymal-like, whereas with E260A were epithelial-like, as treated MMP inhibitor (GM6001). Expression E-cadherin, β-catenin, zonula...

Recent studies have identified a role for ALKBH7 in the occurrence and progression of cancer, this protein is related to cellular immunity immune cell infiltration. However, prognostic immunotherapeutic value different cancers not been explored. In study, we observed high expression 17 low 5 compared paired normal tissues. Although did correlate relatively significantly with clinical parameters age (6/33), sex (3/33) stage (3/27) studied, results survival analysis reflect pan-cancer ALKBH7....

Ferroptosis is a novel type of iron- and ROS-dependent cell death involved in various diseases. LncRNAs are play important roles the occurrence development several cancers. However, researches about role ferroptosis-related lncRNAs glioma relatively rare. Here, we identified nine then constructed prognostic model by LASSO Cox analysis. The could predict overall survival with high sensitivity specificity according to ROC curves. In addition, cycle, p53 signaling, apoptosis, oxidative...

The molecular mechanisms of ovarian cancer cell invasion under hypoxia remain unclear. Here we employed a 3D collagen model and chick chorioallantoic membrane (CAM) assay to explore the influence on invasion. Hypoxia (both 1% O 2 CoCl 150 250 µM) induced HO-8910PM in collagenolysis determined by hydroxyproline. Pretreatment with inducible factor-1α inhibitor, YC-1, or MMP GM6001, significantly inhibited degradation proliferation. stimulated both mRNA protein expressions membrane-type 1...

Glioblastoma is one of the most malignant brain cancers in adults, and it a fatal disease because its untimely pathogenetic location detection, infiltrative growth, unfavorable prognosis.Unfortunately, multimodal treatment with maximal safe resection, chemotherapy radiation has not increased survival rate patients glioblastoma.Gene-and molecular-targeted therapy considered to be promising anticancer strategy for glioblastoma.The identification novel potential targets glioblastoma high...

Background. Human Schlafen 5 (SLFN5) is reported to inhibit or promote the proliferation of several specific types cancer cells by our lab and other researchers. We are curious about its implications in lung adenocarcinoma (LUAC), a malignant tumor with high incidence rate mortality. Method. Lentiviral stable transfections SLFN5-specific shRNA for knockdown SLFN5 full-length coding sequence overexpression were performed LUAC cell analysis vitro vivo nude mice. Clinical samples collected...

Abstract Background Cancer associated fibroblasts (CAFs) communicate metabolically with tumor genesis and development. Rocuronium bromide (RB) is reported to exert certain inhibitory effect on tumor. Here, we investigate the role of RB in esophageal cancer (EC) malignant progression. Methods Tumor xenograft models EC cells were locally systemically administrated detect influence different administrations Mouse CAFs PDGFRα + /F4/80 − sorted by Flow cytometry specific antibodies. treated...

Abstract We previously reported that membrane type‐1 matrix metalloproteinase (MT1‐MMP) enables mesenchymal stem cells (MSCs) to move through both three‐dimensional (3D) type I collagen and basement barriers; however, its upstream regulating factors were unidentified. Here, we report PDGF‐BB upregulates mRNA protein expression of snail in rat bone marrow MSCs (rBMMSCs). enhances rBMMSC invasion 3D collagen, which is blocked by specific siRNA transfection. Snail overexpression induced plasmid...

Amyotrophic lateral sclerosis (ALS) is an incurable neurodegenerative disease that primarily affects motor neurons, causing muscle atrophy, bulbar palsy, and pyramidal tract signs. However, the aetiology pathogenesis of ALS have not been elucidated to date. In this study, a competitive endogenous RNA (ceRNA) network was constructed by analyzing expression profiles messenger RNAs (mRNAs) long noncoding (lncRNAs) were matched 7 samples 4 control samples, then protein-protein interaction (PPI)...

The activation of AKT is one the causes resistance to epidermal growth factor receptor (EGFR)- tyrosine kinase inhibitors (TKIs). Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) combines with related receptors trigger apoptosis or protect cells against TRAIL apoptosis. This research focused on association EGFR and KRAS mutations expression AKT, p-AKT, DR5 DcR1 in non-small cell lung cancer. 82 NSCLC patients were included study. xTAG liquichip techonolgy (xTAG-LCT) was...

Myocardial infarction (MI) is one of the deadliest diseases in world, and changes at molecular level after MI DNA methylation features are not clear. Understanding characteristics early stages significance for treatment disease. In this study, RNA-seq MeDIP-seq were performed on heart tissue from mouse models multiple time points (0 h, 10 min, 1, 6, 24, 72 h) to explore genetic epigenetic that influence progression. Analysis based a single point time, number differentially expressed genes...

Metformin is widely used in the treatment of type 2 diabetes (T2D) and plays a role antitumor antiobesity processes. A recent study identified its direct molecular target, PEN2 (PSENEN). PSENEN minimal subunit multiprotein complex γ-secretase, which promotes differentiation oligodendrocyte progenitors into astrocytes central nervous system. This was mainly based on gene expression data clinical from TCGA CGGA databases. Analysis differential between tissues 31 cancers paracancerous revealed...

Alzheimer's disease (AD) is a progressive and debilitating neurodegenerative disorder prevalent among older adults. Although AD symptoms can be managed through certain treatments, advancing the understanding of underlying mechanisms developing effective therapies critical. In this study, we systematically analyzed transcriptome data from temporal lobes healthy individuals patients with to investigate relationship between mitochondrial autophagy. Machine learning algorithms were used identify...