A5071787851- Physiological and biochemical adaptations
- Aquaculture Nutrition and Growth
- Hemoglobin structure and function
- Genetics, Aging, and Longevity in Model Organisms
- Aquaculture disease management and microbiota
- Adipose Tissue and Metabolism
- Lipid Membrane Structure and Behavior
- Animal Genetics and Reproduction
- Neurobiology and Insect Physiology Research
- Reproductive biology and impacts on aquatic species
- Erythrocyte Function and Pathophysiology
- Advanced biosensing and bioanalysis techniques
- Lipid metabolism and biosynthesis
- thermodynamics and calorimetric analyses
- Animal Behavior and Reproduction
- Gene expression and cancer classification
- Ion Transport and Channel Regulation
- Molecular Biology Techniques and Applications
- Zebrafish Biomedical Research Applications
- RNA Research and Splicing
- Environmental Toxicology and Ecotoxicology
- Bat Biology and Ecology Studies
- Biosensors and Analytical Detection
- Neuroscience of respiration and sleep
- Insect and Arachnid Ecology and Behavior
University of Liverpool
2013-2022
Genomics (United Kingdom)
2013
Heinrich Heine University Düsseldorf
2012
Osnabrück University
2012
Düsseldorf University Hospital
2012
University of Pittsburgh
2012
University of Southern California
2007-2008
University of Copenhagen
2008
National University of Singapore
2008
Keele University
2008
How do organisms respond adaptively to environmental stress? Although some gene-specific responses have been explored, others remain be identified, and there is a very poor understanding of the system-wide integration response, particularly in complex, multitissue animals. Here, we adopt transcript screening approach explore mechanisms underpinning major, whole-body phenotypic transition vertebrate animal that naturally experiences extreme stress. Carp were exposed increasing levels cold,...
Cryptic genetic variation (CGV) is the hidden that can be unlocked by perturbing normal conditions. CGV drive emergence of novel complex phenotypes through changes in gene expression. Although our theoretical understanding has thoroughly increased over past decade, insight into polymorphic expression regulation underlying scarce. Here we investigated transcriptional architecture response to rapid temperature nematode Caenorhabditis elegans. We analyzed regulatory (and mapped eQTL) across...
Extended breath-hold endurance enables the exploitation of aquatic niche by numerous mammalian lineages and is accomplished elevated body oxygen stores adaptations that promote their economical use. However, little known regarding molecular evolutionary underpinnings high muscle myoglobin concentration phenotype divers. We used ancestral sequence reconstruction to trace evolution this oxygen-storing protein across a 130-species phylogeny reveal an adaptive signature net surface charge in...
Poikilothermic animals respond to chronic cold by increasing phosphoglyceride unsaturation restore the fluidity of cold-rigidified membranes. Despite importance this compensatory response, enzymes involved have not been clearly identified, and mechanisms that control their activity are unknown. In carp liver, induces an 8- 10-fold increase in specific microsomal stearoyl coenzyme A desaturase. Cold-induced up-regulation gene transcription resulted a desaturase transcript amounts after 48 60...
We have reconstructed the events that led to evolution of a key physiological innovation underpinning large adaptive radiation fishes, namely their unique ability secrete molecular oxygen (O 2 ). show O secretion into swimbladder evolved some 100 million years after another -secreting system in eye. unravel likely sequence which functional components both systems evolved. These include ocular and countercurrent exchangers, Bohr Root effects, buffering power surface histidine content...
The "fluidity" of brain synaptosomal membrane preparations arctic and hot-springs fish species, two temperature water species acclimated to different seasonal temperatures, mammals was estimated using the fluorescence polarization technique. At all measurement fluidity decreased in order: sculpin, 5 degrees-acclimated goldfish, 25 desert pupfish, rat. This correlated with increasing adaptation or body (i.e., cellular) temperatures 0 degrees, 34 37 degrees suggested a partial compensation for...
Hypoxic vasodilation is a physiological response to low oxygen tension that increases blood supply match metabolic demands. Although this has been characterized for >100 years, the underlying hypoxic sensing and effector signaling mechanisms remain uncertain. We have shown deoxygenated myoglobin in heart can reduce nitrite nitric oxide (NO·) thereby contribute cardiomyocyte NO· during ischemia. On basis of recent observations expressed vasculature hypoxia-tolerant fish, we hypothesized...
ABSTRACT Teleost species from cold environments possess more disordered brain synaptic membranes than warm habitats, thereby providing equivalent physical structures at their respective habitat temperatures. We have related this adaptive interspecific biophysical response to the fatty acid composition of 17 teleost obtained Antarctic, temperate and semi-tropical waters, as well rat turkey representative homeotherms. Cold-adaptive increases in membrane disorder (determined by fluorescence...
Hibernation is a seasonally entrained and profound phenotypic transition to conserve energy in winter. It involves significant biochemical reprogramming, although our understanding of the underpinning molecular events fragmentary selective. We have conducted large-scale gene expression screen golden-mantled ground squirrel, Spermophilus lateralis, identify transcriptional responses associated specifically with summer-winter torpid-arousal used 112 cDNA microarrays comprising 12,288 probes...
Myoglobin (Myg) is an oxygen-binding hemoprotein that widely thought to be expressed exclusively in oxidative skeletal and cardiac myocytes, where it plays a key role coping with chronic hypoxia. We now show hypoxia-tolerant fish model, Myg also range of other tissues, including liver, gill, brain. Moreover, expression transcript was substantially enhanced during hypoxia, the fold-change induction being far greater liver than muscle. By using 2D gel electrophoresis, we have confirmed...
We investigated the effects of fluoxetine, a selective serotonin reuptake inhibitor, on neuroendocrine function and reproductive axis in female goldfish. Fish were given intraperitoneal injections fluoxetine twice week for 14 days, resulting five 5 μg fluoxetine/g body wt. measured monoamine neurotransmitters serotonin, dopamine, norepinephrine addition to their metabolites with HPLC. Homovanillic acid, metabolite dopaminergic pathway, increased significantly hypothalamus. Plasma estradiol...
Abstract The Hawaiian strain (CB4856) of Caenorhabditis elegans is one the most divergent from canonical laboratory N2 and has been widely used in developmental, population, evolutionary studies. To enhance utility strain, we have generated a draft sequence CB4856 genome, exploiting variety resources strategies. When compared against reference, genome 327,050 single nucleotide variants (SNVs) 79,529 insertion–deletion events that result total 3.3 Mb missing 1.4 present but not N2. As...
In 2014, Western Africa experienced an unanticipated explosion of Ebola virus infections. What distinguishes fatal from non-fatal outcomes remains largely unknown, yet is key to optimising personalised treatment strategies. We used transcriptome data for peripheral blood taken infected and convalescent recovering patients identify early stage host factors that are associated with acute illness those differentiate patient survival fatality.The demonstrate individuals who succumbed the disease...
We report a microarray format for the detection of proteins and protein functionality (kinase activity) based on marking either specific antibody−protein binding or peptide phosphorylation events by attachment gold nanoparticles followed silver deposition signal enhancement. The is achieved standard avidin−biotin chemistry. principle resonance light scattering. Highly selective recognition (proteins A G) down to 1 pg/mL in solution 10 fg spots demonstrated. Enzyme activity kinase (PKA)...
Persistent ecotoxicants, such as dioxin and PCBs, are thought to pose one of the greatest threats public ecological health in industrial world. These compounds cause a range macroscopic malformations, particularly craniofacial apparatus cardiovascular system during vertebrate development. However, little is known about microscopic effects, especially on sensitive early life stages or molecular basis developmental neurotoxicity. Using zebrafish (Danio rerio), we have explored neurological...