A5076778055- Plant Pathogens and Fungal Diseases
- Plant Physiology and Cultivation Studies
- Fungal Plant Pathogen Control
- Horticultural and Viticultural Research
- Plant Disease Resistance and Genetics
- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Plant Reproductive Biology
- Postharvest Quality and Shelf Life Management
- Powdery Mildew Fungal Diseases
- Banana Cultivation and Research
- Plant pathogens and resistance mechanisms
- Plant Virus Research Studies
- Genetic Mapping and Diversity in Plants and Animals
- Plant Pathogens and Resistance
- Plant tissue culture and regeneration
- Insect-Plant Interactions and Control
- Yeasts and Rust Fungi Studies
- Garlic and Onion Studies
- Organic Food and Agriculture
- Sustainable Agricultural Systems Analysis
- Genetic and phenotypic traits in livestock
- Genetically Modified Organisms Research
- Plant Gene Expression Analysis
- Plant and animal studies
Agroscope
2016-2025
ETH Zurich
2002-2023
Vifor Pharma (Switzerland)
2011-2012
University of Milan
2008-2009
Walloon Agricultural Research Centre
2007
Swiss Integrative Center for Human Health
2006
University of Bologna
2004-2006
École Polytechnique Fédérale de Lausanne
2004-2005
Board of the Swiss Federal Institutes of Technology
1999-2002
The Vf gene from the wild species Malus floribunda 821 is most studied apple scab resistance gene. Several molecular markers mapping around this were starting point for a positional cloning project. analysis of bacterial artificial chromosome clones spanning region led to identification cluster genes homologous Cladosporium fulvum family tomato. One these genes, HcrVf2 (homologue C. region), was used transform susceptible cultivar Gala. Four independent transformed lines resistant produced,...
Abstract Apple scab caused by Venturia inaequalis has evoked the interest for quite different reasons of scientists, agronomists, producers and consumers since over a century. Consumers select spotless apples, want to avoid damage, agronomists are asked develop implement control measures mostly based on fungicides, scientists challenged find cheaper less questioned measures. Under these premises high number publications have appeared dealing with almost all aspects interaction V....
• Breeding of apple (Malus × domestica) remains a slow process because protracted generation cycles. Shortening the juvenile phase to achieve introgression traits from wild species into prebreeding material within reasonable time frame is great challenge. In this study, we evaluated early flowering transgenic lines overexpressing BpMADS4 gene silver birch with regard tree morphology in glasshouse conditions. Based on results obtained, line T1190 was selected for further analysis and...
Scab caused by the fungal pathogen Venturia inaequalis is most common disease of cultivated apple (Malus × domestica Borkh.). Monogenic resistance against scab found in some small-fruited wild Malus species and has been used breeding for resistance. Vf floribunda 821 widely source. Because a high-quality cultivar perennial fruit trees takes dozens years, cloning genes using them transformation varieties would be advantageous. We report identification cluster receptor-like with homology to...
The generation and selection of novel fire blight resistant apple genotypes would greatly improve the management this devastating disease, caused by Erwinia amylovora. Such are currently developed conventional breeding, but breeding technologies including cisgenesis could be an alternative approach. A cisgenic line C44.4.146 was regenerated using cisgene FB_MR5 from wild Malus ×robusta 5 (Mr5), previously established method involving A. tumefaciens-mediated transformation susceptible...
Summary The fire blight susceptible apple cultivar Malus × domestica Borkh. cv. ‘Gala’ was transformed with the candidate resistance gene FB _ MR 5 originating from crab accession robusta (Mr5). A total of five different transgenic lines were obtained. All shown to be stably and originate events. express either driven by constitutive Ca MV 35S promoter ocs terminator or its native sequences. Phenotyping experiments performed Mr5‐virulent Mr5‐avirulent strains Erwinia amylovora , causal agent...
Apple scab, caused by Venturia inaequalis, is a major fungal disease worldwide. Cultivation of scab-resistant cultivars would reduce the chemical footprint apple production. However, new carrying durable resistances should be developed to prevent or at least slow breakdown resistance against races V. inaequalis. One way achieve pyramid multiple scab genes in cultivar. The choice combined pyramids take into account frequency and geographical distribution isolates able cause such breakdowns....
Texture is a complex trait and major component of fruit quality in apple. While the effect
Abstract Breeding of apple is a long-term and costly process due to the time space requirements for screening selection candidates. Genomics-assisted breeding utilizes genomic phenotypic information increase efficiency in programs, measurements phenotypes different environments can facilitate application approach under various climatic conditions. Here we present an reference population: REFPOP, large collection formed 534 genotypes planted six European countries, as unique tool accelerate...
Abstract Implementation of genomic tools is desirable to increase the efficiency apple breeding. Recently, multi-environment reference population (apple REFPOP) proved useful for rediscovering loci, estimating predictive ability, and studying genotype by environment interactions (G × E). So far, only two phenological traits were investigated using REFPOP, although may be valuable when dissecting genetic architecture reporting abilities additional key in Here we show contrasting 30...
Abstract A polymerase chain reaction (PCR)‐based method was developed to isolate microsatellite markers from large‐insert genomic DNA clones of bacterial artificial chromosome (BAC) libraries. The is fast and economic since it does not require subcloning. It applied a marker BAC clone the chromosomal region containing apple scab resistance gene Vf . Malus floribunda 821 most widely used source in breeding. second found on extremity flanking locus. two microsatellites allowed identification...
A fire blight resistance QTL explaining 34.3%-46.6% of the phenotypic variation was recently identified on linkage group 7 apple cultivar 'Fiesta' (F7). However, markers flanking this were AFLP and RAPD unsuitable for marker-assisted selection (MAS). Two bracketing have been transformed into SCAR (sequence-characterized amplified region) markers, an SSR marker specific region developed. Pedigree analysis with these enabled tracking F7 allele back to 'Cox's Orange Pippin'. Stability effect in...
Fire blight is a devastating bacterial disease of rosaceous plants. Its damage to apple production major concern, since no existing control option has proven be completely effective. Some commercial varieties, such as ‘Florina’ and ‘Nova Easygro’, exhibit consistent level resistance fire blight. In this study, we used an F 1 progeny × Easygro’ build parental genetic maps identify quantitative trait loci (QTLs) related resistance. Linkage were constructed using set microsatellites enriched...