A5003311318- Parkinson's Disease Mechanisms and Treatments
- Alzheimer's disease research and treatments
- Supramolecular Self-Assembly in Materials
- Protein Hydrolysis and Bioactive Peptides
- Enzyme Production and Characterization
- Quantum Dots Synthesis And Properties
- Conducting polymers and applications
- Botulinum Toxin and Related Neurological Disorders
- Neurological diseases and metabolism
- Advanced Sensor and Energy Harvesting Materials
- Proteins in Food Systems
- Polydiacetylene-based materials and applications
- Polymer Surface Interaction Studies
- Gold and Silver Nanoparticles Synthesis and Applications
- biodegradable polymer synthesis and properties
- Phytase and its Applications
- Genetic Neurodegenerative Diseases
- Nuclear Receptors and Signaling
- ATP Synthase and ATPases Research
- Antimicrobial Peptides and Activities
- Nanoplatforms for cancer theranostics
- Electrochemical sensors and biosensors
- Neuroscience and Neural Engineering
- Nanocluster Synthesis and Applications
- Nerve injury and regeneration
Seoul National University
2015-2025
Bioengineering (Switzerland)
2025
Biological E (India)
2024
Institute of Engineering
2024
Seoul National University of Education
2011
Yonsei University
2002-2006
Korea Advanced Institute of Science and Technology
2006
Inha University
1997-2004
University Gastroenterology
1995
Samsung Medical Center
1995
Ni/NiO core/shell nanoparticles having high affinity with polyhistidine were synthesized by decomposition of a Ni surfactant complex followed air oxidation. showed selective and efficient binding to histidine-tagged proteins easy separation using magnet. These provided more convenient way purification compared the conventional Ni−NTA complex-bound resins microbeads.
alpha-Synuclein is a component of the abnormal protein depositions in senile plaques and Lewy bodies Alzheimer's disease (AD) Parkinson's respectively. The was suggested to provide possible nucleation centre for plaque formation AD via selective interaction with amyloid beta/A4 (Abeta). We have shown previously that alpha-synuclein has experienced self-oligomerization when Abeta25-35 present an orientation-specific manner sequence. Here we examine this biochemically specific use various...
An investigation was carried out on an oxidative and SDS-stable alkaline protease secreted by Bacillus clausii of industrial significance.Maximum enzyme activity produced when the bacterium grown in medium containing (g l-1): soyabean meal, 15; wheat flour, 10; liquid maltose, 25; K2HPO4, 4; Na2HPO4, 1; MgSO4.7H2O, 0.1; Na2CO3, 6. The has optimum pH around 11 temperature 60 degrees C. showed extreme stability towards SDS oxidizing agents, which retained its above 75 110% treatment for 72 h...
The presynaptic α-synuclein is a prime suspect for contributing to Lewy pathology and clinical aspects of diseases, including Parkinson's disease, dementia with bodies, body variant Alzheimer's disease. Here we examined the pathogenic mechanism neuronal cell death induced by α-synuclein. exogenous addition caused marked decrease viability in primary immortalized cells. appeared be correlated Rab5A-specific endocytosis that subsequently formation body-like intracytoplasmic inclusions. This...
A magnetically recyclable protein separation system has been designed and fabricated by combining ferrimagnetic magnetite cores NiO nanoparticles decorated onto the mesoporous silica shell with a high surface area (see figure). The exposed provide for selective adsorption of His-tagged from mixed-protein solution, as well cell lysate, magnetic core allows particles to be separated solution applying an external field. Detailed facts importance specialist readers are published "Supporting...
Abstract A single amyloidogenic protein is implicated in multiple neurological diseases and capable of generating a number aggregate “strains” with distinct structures. Among the proteins, α-synuclein generates patterns proteinopathies group diseases, such as Parkinson disease (PD), dementia Lewy bodies (DLB), system atrophy (MSA). However, link between specific conformations pathologies, key concept strain hypothesis, remains elusive. Here we show that presence bacterial endotoxin,...
Aggregation of α-synuclein is thought to play a major role in the pathogenesis Parkinson's disease (PD), which characterized by presence intracytoplasmic Lewy bodies (LB) brain. α-Synuclein and its deletion mutants are largely unfolded proteins with random coil structures as revealed CD spectra, fluorescence gel filtration chromatography, ultracentrifugation. On basis highly flexible conformation, we have investigated chaperone-like activity vitro. In our experiments, inhibited aggregation...
Gathering evidence has associated activation of microglia with the pathogenesis numerous neurodegenerative diseases central nervous system (CNS) such as Alzheimer's disease and Parkinson's disease. Microglia are resident macrophages CNS whose functions include chemotaxis, phagocytosis, secretion a variety cytokines proteases. In this study, we examined possibility that alpha-synuclein (alpha-syn), which is disease, may affect phagocytic function microglia. We found extracellular monomeric...
Abstract Many lines of evidence suggest that α‐synuclein can be secreted from cells and penetrate into them, although the detailed mechanism is not known. In this study, we investigated amino acid sequence motifs required for membrane translocation α‐synuclein, mechanistic features phenomenon. We first showed only but also β‐ γ‐synucleins penetrated live cells, indicating conserved N‐terminal region might responsible translocation. Using a series deletion mutants, demonstrated 11‐amino...
Cell-mediated nanoparticle delivery has been proposed for an effective cancer therapy. However, there are limitations in loading nanoparticles within cells as the internalized cause cytotoxicity and leak out of via exocytosis. Here, we introduce hybrid sheets composed gold (AuNPs) graphene oxide (GO), which stably adhere to cell surface exhibit a remarkable photothermal effect. To form AuNP/GO GO is sandwiched between two AuNP monolayers, AuNPs coated with α-synuclein protein subsequently...
Abstract α‐Synuclein, a major constituent of Lewy bodies (LBs) in Parkinson's disease (PD), has been implicated to play critical role synaptic events, such as neuronal plasticity during development, learning, and degeneration under pathological conditions, although the physiological function α‐synuclein not yet established. We here present biochemical evidence that recombinant chaperone‐like against thermal chemical stress vitro. In our experiments, protected glutathione S‐transferase (GST)...
Lewy bodies (LBs) are pathological hallmarks of Parkinson disease (PD) but also occur in Alzheimer (AD) and dementia LBs. Alpha-synuclein, the major component LBs, is observed brain Down syndrome (DS) patients with AD. Dyrk1A, a dual specificity tyrosine-regulated kinase (Dyrk) family member, mammalian ortholog Drosophila minibrain (Mnb) gene, essential for normal postembryonic neurogenesis. The Dyrk1A gene resides human chromosome 21q22.2 region, which associated DS anomalies, including...
Parkinson disease (PD) is a common neurodegenerative disorder, which involves the deterioration of dopaminergic neurons in pars compacta substantia nigra. The etiology PD still unknown, but recent identification mutations familial cases has advanced understanding molecular mechanisms this neurological disease. Mutations parkin gene, encodes for ubiquitin-protein ligase (E3), have been implicated autosomal recessive juvenile Parkinsonism, an early onset and form PD. Here we reported that...
Background: A prion-like spread of α-synuclein might play a role in the pathogenesis Parkinson disease.Results: Extracellular was cleaved by plasmin. Cultured microglia and astrocytes did not take up plasmin digested extracellular α-synuclein, were activated.Conclusion: Plasmin-mediated clearance problems disease.Significance: Therapies aimed at may lead to new therapies for disease. Results: activated. Conclusion: Significance:
(-)-Epigallocatechin gallate (EGCG), the major component of green tea, has been re-evaluated with α-synuclein (αS), a pathological constituent Parkinson's disease, to elaborate its therapeutic value. EGCG demonstrated not only induce off-pathway 'compact' oligomers αS as suggested previously, but also drastically enhance amyloid fibril formation αS. Considering that EGCG-induced fibrils could be product on-pathway SDS-sensitive 'transient' oligomers, polyphenol effect on transient 'active'...
α-Synuclein, a presynaptic protein of the central nervous system, has been implicated in synaptic events such as neuronal plasticity during development and learning, degeneration under pathological conditions. As an effort to understand biological function α-synuclein, we examined expression patterns α-synuclein various human hematopoietic cells, Drosophila at different developmental stages. The was ubiquitously expressed all tested cells including T B NK monocytes, well lymphoma cell lines,...