A5056480472- Nanoparticle-Based Drug Delivery
- Seaweed-derived Bioactive Compounds
- Hemoglobin structure and function
- Acute Ischemic Stroke Management
- Cerebrovascular and Carotid Artery Diseases
- Intracranial Aneurysms: Treatment and Complications
- Ultrasound and Hyperthermia Applications
- Protease and Inhibitor Mechanisms
- Erythrocyte Function and Pathophysiology
- Advanced Polymer Synthesis and Characterization
- RNA Interference and Gene Delivery
- Nanoparticles: synthesis and applications
- Photoacoustic and Ultrasonic Imaging
- Extracellular vesicles in disease
- Electrospun Nanofibers in Biomedical Applications
- Neonatal Health and Biochemistry
- Connective tissue disorders research
- Nanoplatforms for cancer theranostics
- Aortic aneurysm repair treatments
- Angiogenesis and VEGF in Cancer
- Graphene and Nanomaterials Applications
- Blood Coagulation and Thrombosis Mechanisms
- Polymer Surface Interaction Studies
- Proteoglycans and glycosaminoglycans research
- Hemostasis and retained surgical items
Université Paris Cité
2015-2025
Laboratoire de Recherche Vasculaire Translationnelle
2014-2025
Université Sorbonne Paris Nord
2015-2025
Sorbonne Université
2009-2025
Inserm
2015-2025
Délégation Paris 7
2014-2018
Université Claude Bernard Lyon 1
2014-2018
Hôpital Bichat-Claude-Bernard
2018
Assistance Publique – Hôpitaux de Paris
2018
Sorbonne Paris Cité
2014-2017
Among cardiovascular diseases, thrombotic diseases such as ischemic heart disease and acute strokes are the most lethal, responsible by themselves for a quarter of worldwide deaths. While surgical treatments exist, they may not be used in all situations, systemic thrombolytic drug injection, recombinant tissue plasminogen activators (rtPA), often remains necessary, despite serious limitations including short therapeutic window, severe side effects, failure to address complex nature thrombi....
Aim: We have designed ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles associated with fucoidan (USPOI-FUCO), a natural sulfated polysaccharide high affinity for activated platelets, to visualize by MRI arterial thrombi. Materials & methods: USPIOs were prepared and sizes, zeta-potentials relaxivities measured. Elastase perfusion in the infrarenal aorta of Wistar rats induced intraluminal thrombus. They scanned on 4.7 T before after injection USPIO-FUCO or USPIO coated anionic...
Tissue plasminogen activator (tPA) is the sole approved therapeutic molecule for treatment of acute ischemic stroke. Yet, only a small percentage patients could benefit from this life-saving because medical contraindications and severe side effects, including brain hemorrhage, associated with delayed administration. Here, nano agent realized by directly associating clinical formulation tPA to porous structure soft discoidal polymeric nanoconstructs (tPA-DPNs). The matrix DPNs protects rapid...
We report the physicochemical analysis of nanosystems intended for cardiovascular applications and their toxicological characterization in static dynamic cell culture conditions.Size, polydispersity ζ-potential were determined 10 nanoparticle systems including liposomes, lipid nanoparticles, polymeric iron oxide nanoparticles. Nanoparticle effects on primary human endothelial viability monitored using real-time live-cell microscopy conditions, a flow model arterial bifurcations.The majority...
Fucoidans constitute a large family of sulfated polysaccharides with several biochemical properties. A commercial fucoidan from brown algae, containing low molecular weight polysaccharidic species constituted l-fucose, uronic acids and sulfate groups, was simply treated here calcium acetate solution. This treatment led to purified fraction yield 45%. The physicochemical characterizations the using colorimetric assay, MALLS, dRI, FT-IR, NMR, exhibited distributions chemical profiles similar...
A radical emulsion polymerization of alkylcyanoacrylates (ACA) has been investigated to produce core−shell nanoparticles coated with polysaccharides. It was initiated by dextran on which a created reaction cerium(IV) ions in an aqueous acidic medium. The redox developed this study occurred much faster at pH 1 than the spontaneous anionic ACA. applied several ACA and led formation very stable suspensions nanoparticles. mean diameter depended monomer. Different analysis showed that polymer...
The oligomeric state and kinetics of ligand binding were measured for wild‐type cytoglobin. Cytoglobin has the classical globin fold, with an extension at each extremity about 20 residues. extended length cytoglobin leads to ambiguous interpretation its state. Although hydrodynamic diameter corresponds that a dimer, it displays mass single subunit, indicating monomeric form. Thus, rather than displaying compact globular form, behaves hydrodynamically like tightly packed greater flexibility...
New tools for molecular imaging and targeted therapy cardiovascular diseases are still required. Herein, biodegradable microcapsules (MCs) made of polycyanoacrylate polysaccharide functionalized with fucoidan (Fuco‐MCs) designed as new carriers to target arterial thrombi overexpressing P‐selectin. Physicochemical characterizations demonstrated that have a core–shell structure is present onto the surface Fuco‐MCs. Furthermore, their sizes range from 2 6 µm they stable on storage over 30 d at...
The oligomeric state and kinetics of ligand binding were measured for wild-type cytoglobin. Cytoglobin has the classical globin fold, with an extension at each extremity about 20 residues. extended length cytoglobin leads to ambiguous interpretation its state. Although hydrodynamic diameter corresponds that a dimer, it displays mass single subunit, indicating monomeric form. Thus, rather than displaying compact globular form, behaves hydrodynamically like tightly packed greater flexibility...
Aneurysm diagnostic is nowadays limited by the lack of technology that enables early detection and rupture risk prediction.New non invasive tools for molecular imaging are still required.In present study, we an innovative SPECT tool abdominal aortic aneurysm (AAA) produced from injectable polysaccharide microparticles radiolabeled with technetium 99m ( Tc) functionalized fucoidan, a sulfated ability to target P-Selectin.P-Selectin cell adhesion molecule expressed on activated endothelial...
Herein we investigate the structure/function relationships of fucoidans from Ascophyllum nodosum to analyze their pro-angiogenic effect and cellular uptake in native glycosaminoglycan-free (GAG-free) human endothelial cells (HUVECs). Fucoidans are marine sulfated polysaccharides, which act as glycosaminoglycans mimetics. We hypothesized that size sulfation rate influence ability induce processes independently GAGs. collected two fractions fucoidans, Low Medium Molecular Weight Fucoidan (LMWF...