A5060566499- Acute Ischemic Stroke Management
- Blood Coagulation and Thrombosis Mechanisms
- Nanoplatforms for cancer theranostics
- Nanoparticle-Based Drug Delivery
- Protease and Inhibitor Mechanisms
- Cell Adhesion Molecules Research
- Platelet Disorders and Treatments
- Seaweed-derived Bioactive Compounds
- Aortic aneurysm repair treatments
- Connective tissue disorders research
- Angiogenesis and VEGF in Cancer
- Cerebrovascular and Carotid Artery Diseases
- Antiplatelet Therapy and Cardiovascular Diseases
- Characterization and Applications of Magnetic Nanoparticles
- Extracellular vesicles in disease
- X-ray Diffraction in Crystallography
- Blood properties and coagulation
- Aortic Disease and Treatment Approaches
- Atherosclerosis and Cardiovascular Diseases
- Neuroinflammation and Neurodegeneration Mechanisms
- Crystallization and Solubility Studies
- Venous Thromboembolism Diagnosis and Management
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Hydrogels: synthesis, properties, applications
- Proteoglycans and glycosaminoglycans research
Inserm
2014-2024
Université de Caen Normandie
2019-2024
Normandie Université
2019-2024
Physiopathologie et imagerie des troubles neurologiques
2019-2024
Australian Centre for HIV and Hepatitis Virology Research
2017-2024
Cyceron
2021-2024
Centre Hospitalier Universitaire de Caen Normandie
2023
Hypertension pulmonaire : physiopathologie et innovation thérapeutique
2022
Monash University
2017-2022
Utrecht University
2021
Therapeutic nanoparticles hold clinical promise for cancer treatment by avoiding limitations of conventional pharmaceuticals. Herein, a facile and rapid method is introduced to assemble poly(ethylene glycol) (PEG)-modified Pt prodrug nanocomplexes through metal-polyphenol complexation combined with emulsification, which results in ≈100 nm diameter (PtP NPs) that exhibit high drug loading (0.15 fg per nanoparticle) low fouling properties. The PtP NPs are characterized potential use as...
Smart poly(2-oxazoline) (POx)-based multifunctional polymer capsules that specifically target glycoprotein (GP) IIb/IIIa on the surface of activated platelets are degraded by serine protease thrombin and release urokinase plasminogen activator loaded into capsules, only in area acute thrombosis. As a service to our authors readers, this journal provides supporting information supplied authors. Such materials peer reviewed may be re-organized for online delivery, but not copy-edited or...
Abstract In acute ischemic stroke, even when successful recanalization is obtained, downstream microcirculation may still be obstructed by microvascular thrombosis, which associated with compromised brain reperfusion and cognitive decline. Identifying these microthrombi through non-invasive methods remains challenging. We developed the PHySIOMIC (Polydopamine Hybridized Self-assembled Iron Oxide Mussel Inspired Clusters), a MRI-based contrast agent that unmasks microthrombi. mouse model of...
Hydrogen sulfide (H2 S) has been identified as an important cell-signaling mediator and a number of biological functions, such vascular smooth muscle relaxation, neurotransmission, regulation inflammation. A facile versatile approach for H2 S production initiated by light irradiation controlled reaction with amine or amino acid was developed. The donor synthesized in one-pot reaction, simple crystallization led to yield approximately 90 %. synthetic strategy is scalable versatile, the donors...
Molecular magnetic resonance imaging (MRI) holds great promise for diagnosis and therapeutic monitoring in a wide range of diseases. However, the low intrinsic sensitivity MRI to detect exogenous contrast agents lack biodegradable microprobes have prevented its clinical development. Here, we synthetized agent molecular based on previously unknown mechanism self-assembly catechol-coated magnetite nanocrystals into microsized matrix-based particles. The resulting (M3P particles) carry up...
Severe thrombosis and its ischemic consequences such as myocardial infarction, pulmonary embolism stroke are major worldwide health issues. The ferric chloride injury is now a well-established technique to rapidly accurately induce the formation of thrombi in exposed veins or artery small large diameter. This model has played key role study pathophysiology thrombosis, discovery validation novel antithrombotic drugs understanding mechanism action these new agents. Here, implementation this on...
Abstract The objective of this work was to develop a high throughput assay for testing in vitro the thrombolytic activity using citrated whole blood samples, and overcome limitations currently available techniques. We successfully developed method that involves forming halo shaped, tissue factor induced, clots 96 well plates, then precisely measuring thrombolysis process with spectrophotometer plate reader. here describe implementation novel method, which we refer as assay, its validation...
Aneurysm diagnostic is nowadays limited by the lack of technology that enables early detection and rupture risk prediction.New non invasive tools for molecular imaging are still required.In present study, we an innovative SPECT tool abdominal aortic aneurysm (AAA) produced from injectable polysaccharide microparticles radiolabeled with technetium 99m ( Tc) functionalized fucoidan, a sulfated ability to target P-Selectin.P-Selectin cell adhesion molecule expressed on activated endothelial...
Nanomedicine holds great promise for vascular disease diagnosis and specific therapy, yet rapid sequestration by the mononuclear phagocytic system limits efficacy of particle-based agents. The use low-fouling polymers, such as poly(ethylene glycol), efficiently reduces this immune recognition, but these nondegradable polymers can accumulate in human body may cause adverse effects after prolonged use. Thus, new particle formulations combining stealth, low immunogenicity biocompatible features...
Despite advances in antithrombotic therapy, the risk of recurrent coronary/cerebrovascular ischemia or venous thromboembolism remains high. Dual pathway blockade, using both antiplatelet and anticoagulant offers promise improved thrombotic protection; however, widespread adoption tempered by substantial major bleeding. Here, we report a dual therapeutic capable site-specific targeting to activated platelets enrichment at site thrombus growth allow reduced dosing without compromised efficacy....
Monitoring immune reactions via inflammation imaging provides valuable disease prognosis but remains limited by currently available probes and capabilities. In this study, a bimodal probe was developed from microsized polydopamine matrix-based magnetic particles (M3P) functionalized with antibodies that target vascular cell adhesion molecule-1 (VCAM-1) are radiolabeled the radioisotope Gallium-68. This combines sensitive detection Positron Emission Tomography (immuno-PET) high-resolution...
Background Thrombolytic therapy for acute thrombosis is limited by life‐threatening side effects such as major bleeding and neurotoxicity. New treatment options with enhanced fibrinolytic potential are therefore required. Here, we report the development of a new thrombolytic molecule that exploits key features thrombosis. We designed recombinant microplasminogen modified to be activated prothrombotic serine‐protease thrombin (HtPlg), fused an activation‐specific anti–glycoprotein IIb/ III...
Severe thrombosis and its ischemic consequences such as myocardial infarction, pulmonary embolism stroke are major worldwide health issues. The ferric chloride injury is now a well-established technique to rapidly accurately induce the formation of thrombi in exposed veins or artery small large diameter. This model has played key role study pathophysiology thrombosis, discovery validation novel antithrombotic drugs understanding mechanism action these new agents. Here, implementation this on...
Background and Purpose: Brain imaging has become central in the management of acute ischemic stroke. Detection parenchymal injury perfusion enables characterization extent damage, which guides treatment decision-making. Additional assessment secondary events, such as inflammation, may particularly arise after recanalization, improve diagnosis (supplementary) selection. Therefore, we developed tested a molecular magnetic resonance (MRI) approach for vivo detection vascular inflammation...
We report the development of a theranostic collagen targeted cell penetrating drug delivery system toward treatment cardiovascular disease. Caused by action matrix metalloproteinases (MMP), degraded is hallmark unstable atherosclerotic plaques that are highly susceptible rupture. Targeting and delivering MMP blockers directly to plaque inhibit activity promising new strategy requires benefits possibilities nanodelivery approaches. The presented designed (a) target bind cryptic epitope on IV...
Abstract Vascular injury models are indispensable for studying thrombotic processes in vivo. Amongst the available methods inducing thrombosis, laser-induced endothelial (LIEI) has several unique advantages. However, a lack of methodological standardization and expensive instrumentation remain significant problems decreasing reproducibility impeding adoption LIEI wider scientific community. In this, study, we developed standardized protocol scanning (scanning-LIEI) murine mesenteric veins...
Tomographic perfusion imaging techniques are integral to translational stroke research paradigms that advance our understanding of the disease. Functional ultrasound (fUS) is an emerging technique informs on cerebral blood volume (CBV) through ultrasensitive Doppler and flow velocity (CBFv) ultrafast localization microscopy. It not known how experimental results compare with a classical CBV-probing such as dynamic susceptibility contrast-enhanced MRI (DSC-MRI). To end, we assessed...