A5100778681- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Renal and related cancers
- RNA Research and Splicing
- Electrocatalysts for Energy Conversion
- Innovation and Socioeconomic Development
- interferon and immune responses
- RNA regulation and disease
- Nanoplatforms for cancer theranostics
- Skin Protection and Aging
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Reproductive Biology and Fertility
- Wound Healing and Treatments
- Congenital heart defects research
- Graphene and Nanomaterials Applications
- Tissue Engineering and Regenerative Medicine
- Genomics and Chromatin Dynamics
- Receptor Mechanisms and Signaling
- Metalloenzymes and iron-sulfur proteins
- Phytochemistry and Biological Activities
- Ammonia Synthesis and Nitrogen Reduction
- Antibiotic Resistance in Bacteria
- Fuel Cells and Related Materials
- RNA modifications and cancer
The Seventh Affiliated Hospital of Sun Yat-sen University
2024
Sun Yat-sen University
2024
First Bethune Hospital of Jilin University
2023
Guangzhou University of Chinese Medicine
2023
Harbin Medical University
2022
Guangdong Academy of Medical Sciences
2020-2021
Southern Medical University
2020-2021
Guangdong Provincial People's Hospital
2020-2021
Jinan Central Hospital
2019
Shandong University
2019
Loss of c-JUN leads to early mouse embryonic death, possibly because a failure develop normal cardiac system. How regulates human cardiomyocyte cell fate remains unknown. Here, we used the in vitro differentiation pluripotent stem cells into cardiomyocytes study role c-JUN. Surprisingly, knockout improved generation, as determined by number TNNT2+ cells. ATAC-seq data showed that defect led increased chromatin accessibility on critical regulatory elements related development. ChIP-seq RBBP5...
A growing number of studies have suggested that traditional Chinese medicine (TCM) plays an essential role in the development and occurrence liver cancer. However, function Ruangan Lidan decoction (RLD) cancer are not yet adequately identified manifested, which attracted our attention. The key genes related to RLD upstream miRNAs PDK4 were obtained based on bioinformatics analysis, followed by verification targeting relationship between miR-9-5p PDK4. Next, Huh7 cells treated with detect...
Abstract PI3K signaling pathway plays a significant role in embryonic stem cells (ES cells) self‐renewal. Overexpression of Nanog maintains mouse ES pluripotency independent leukemia inhibitory factor (LIF). However, little is known about the effect on with overexpression. Our experiments aimed to explore relationship between and expression cells. We observed LY294002, specific inhibitor pathway, wild‐type J1 overexpressing (Ex‐Nanog) presence or absence LIF. With LY294002 treatment, both...
Abstract Background Four transcription factors, Oct4, Sox2, Klf4, and c-Myc (the Yamanka factors), can reprogram somatic cells to induced pluripotent stem (iPSCs). Many studies have provided a number of alternative combinations the non-Yamanaka factors. However, it is clear that many additional factors generate iPSCs remain be discovered. Methods The chromatin accessibility transcriptional level human embryonic urine were compared by Assay for Transposase-Accessible Chromatin with...
The global emergence of antibiotic resistance, especially in Gram-negative bacteria, is an urgent threat to public health. Inevitably, considering its extensive use and misuse, resistance toward ciprofloxacin has increased almost all clinically relevant bacteria. This study aimed investigate the transcriptome changes at a high concentration Escherichia coli. In brief, 1,418 differentially expressed genes (DEGs) were identified, from which 773 upregulated by ciprofloxacin, whereas 651...
Methylation is a common modification of DNA. It has been very important and hot topic to study the correlation between methylation diseases in medical science. Because special process with bisulfite treatment, traditional mapping tools do not work well such experimental reads. Traditional aligners are designed for bisulfite-treated reads, where un-methylated 'C's converted 'T's.In this paper, we develop reliable visual tool, named VAliBS, bisulfate sequences genome reference. VAliBS works...
β-地中海贫血是β-珠蛋白基因（HBB）点突变或缺失引起的单基因遗传病，患者产生溶血性贫血，其生存主要依赖于反复输血及去铁治疗，最终导致多器官受损、衰竭，给个人、家庭和国家带来严重的经济负担。随着基因编辑技术的蓬勃发展，可能治愈基因突变性疾病的基因治疗应运而生，其中CRISPR/Cas9基因编辑技术凭其靶向特异性、经济性等优势备受关注，并已广泛应用于分子生物学以及生命科学领域的研究中。如今CRISPR/Cas9基因编辑技术修复人多能干细胞HBB基因、诱导胎儿血红蛋白（HbF）合成或抑制α-珠蛋白基因（HBA基因）表达是治愈β-地中海贫血的三大可行性策略，相关临床试验已相继开展。本文就CRISPR/Cas9系统的研究进展、作用机制以及在治疗β-地中海贫血的最新研究与应用进行综述，涉及细胞实验、动物模型、临床试验等内容，并介绍了CRISPR/Cas9系统衍生的新型基因编辑工具碱基编辑器在该领域的研究应用，旨在为促进β-地中海贫血从基础研究转向临床治疗提供新的思路和方向。
Purpose: Burn is one of the most common injuries in clinical practice. The use transcription factors (TFs) has been reported to reverse epigenetic rewiring process and great promise for skin regeneration. To better identify key TFs reprogramming, we proposed a predictive system that conjoint analyzed gene expression data regulatory network information. Methods: Firstly, tissues were downloaded LIMMA package was used differential-expressed genes (DEGs). Then three ways, including...
Spermatogenesis is sustained by the proliferation and differentiation of spermatogonial stem cells (SSCs). SSCs are derived from primordial germ (PGCs) during embryonic development. The signal mammalian tissue critical to localization PGCs various factors signaling pathways involved into regulation these process. However, molecules controlling processes still need further clarification. In this concise review, origin, development, regulating mechanisms throughout summarized discussed in...