A5074145261- Hepatitis B Virus Studies
- Hepatitis C virus research
- Liver Disease Diagnosis and Treatment
- FOXO transcription factor regulation
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- HIV Research and Treatment
- COVID-19 epidemiological studies
- Nuclear Receptors and Signaling
- Circular RNAs in diseases
- Aldose Reductase and Taurine
- Hepatitis Viruses Studies and Epidemiology
- Pancreatic and Hepatic Oncology Research
- Pediatric health and respiratory diseases
- Vibrio bacteria research studies
- Epigenetics and DNA Methylation
- Histone Deacetylase Inhibitors Research
- COVID-19 diagnosis using AI
- RNA Interference and Gene Delivery
- Phytochemical Studies and Bioactivities
- NF-κB Signaling Pathways
- Environmental Impact and Sustainability
- Heat shock proteins research
People's Hospital of Bishan District
2022-2024
Chongqing Medical University
2011-2024
Chongqing Emergency Medical Center
2018-2023
Chongqing University
2009-2023
Dalian Medical University
2008-2017
Second Affiliated Hospital of Chongqing Medical University
2008-2017
Cedars-Sinai Medical Center
2014-2015
Dongguan People’s Hospital
2011
University of Hong Kong
2006-2007
Hong Kong Metropolitan University
2006
Abstract Background MircoRNAs(miRNAs) are short, endogenously non-coding RNAs. The abnormal expression of miRNAs may be valuable for the diagnosis and treatment tumors. Methods To screening special in gastric carcinoma, level carcinoma normal gaster samples were detected by miRNA gene chip. Then, expressions miR-9 miR-433 tissue SGC7901 cell line validated qRT-PCR. GRB2 RAB34, targets respectively, Western blot. Results We found 19 7 down-regulated up-regulated respectively. Compared with...
Within the family of RTKs (receptor tyrosine kinases), PDGFR (platelet-derived growth factor receptor) has been implicated in carcinogenesis and tumour development. miRNAs (microRNAs), which can target mRNAs (messenger RNAs) cancer-associated genes, are abnormally expressed various cancers. In this study, our aim was to identify that PDGFR-α/β study functions these miRNAs. miR-34a predicted PDGFR, luciferase reporter assays showed could directly PDGFR. Meanwhile, we found down-regulated...
The osmotic response element-binding protein (OREBP), also known as tonicity enhancer-binding (TonEBP) or NFAT5, regulates the hypertonicity-induced expression of a battery genes crucial for adaptation mammalian cells to extracellular hypertonic stress. activity OREBP/TonEBP is regulated at multiple levels, including nucleocytoplasmic trafficking. can be detected in both cytoplasm and nucleus under isotonic conditions, although it accumulates exclusively when subjected hypotonic challenges,...
Metastasis is the major cause of cancer-related death. Forkhead Box M1 (FoxM1) a master regulator tumor metastasis. This study aims to identify new FoxM1 targets in regulating metastasis using bioinformatics tools as well biological experiments. Illumina microarray was used profile WT and PTTG1 knockout HCT116 cells. R2 Genomics Analysis potential targeted gene. Luciferase reporter array, EMSA Chromatin Immunoprecipitation (ChIP) were determine binding promoter. Boyden chamber assay evaluate...
Gastric intestinal metaplasia (GIM), a common precancerous lesion of gastric cancer, can be caused by bile acid reflux. GATA binding protein 4 (GATA4) is an transcription factor involved in the progression cancer. However, expression and regulation GATA4 GIM has not been clarified.
We did a comprehensive exploration of the epidemiological and clinical characteristics 136 patients with confirmed COVID-19 in main district Chongqing which was adjacent to west Hubei province. This study conducted on from Jan 25 Feb 20, 2020. Data included demographic, epidemiological, features, chest radiographs imported cases, local second-generation cases third-generation cases. Student's t-test adopted for quantitative variables while Pearson Chi-squared test or Fisher's exact...
The liver X receptor alpha (LXRα), a member of the nuclear superfamily, has been shown to regulate expression fatty acid synthase (FAS) gene through direct interaction with FAS promoter. However, its regulation is not completely understood. Histone modifications and chromatin remodeling are closely linked transcriptional activation genes. In present study, we examined effect LXRα or silencing on histone (i.e., acetylation, methylation, phosphorylation) across gene, aim investigate whether...
Hepatitis B Virus (HBV) covalently closed circular DNA (cccDNA) is the main replicative intermediate of HBV and organized into minichromosomes by interaction with histone nonhistone proteins. The remodeling such as post-translational modifications proteins plays an important role in regulating replication. To determine whether other occurs addition to acetylation cccDNA-bound H3 histones presence replication, a cell culture replication model was used assess dynamic status acetylation,...
Hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) is organised into minichromosomes by histone and non-histone proteins. Remodelling of crucial for the regulation HBV replication, which dependent on presence hepatitis X protein (HBx). However, mechanisms HBx-dependent replication remain obscure. The objective this study was to investigate mechanism through pathway chromatin remodelling. role HBx investigated transfecting human HepG2 cells with linear full-length genome...
Single nucleotide polymorphisms (SNPs) of HLA-DQ and granulysin (GNLY) are reportedly associated with HBV infection. The aim this study was to investigate the effects interactions between SNPs in GNLY on outcome hepatitis B virus (HBV) infection Chinese Han subjects. (rs9275572) (rs1866139 rs11127) were genotyped 310 subjects HBV-related chronic liver disease, 295 whom spontaneous clearance had occurred 316 who not been exposed HBV. rs9275572 significantly correlated (dominant genetic model:...
Abstract
It is well known that different genotypes of hepatitis B virus (HBV) have a sensitivity to interferon-α or lamivudine (nucleoside analogue) antiviral therapy. However, for adefovir dipivoxil (ADV, nucleotide analogue), the response remains be clarified. In order evaluate HBV ADV therapy and identify factors might affect initial virological response, we performed retrospective analysis on patients with chronic (CHB) in Chinese Han population. The study included 183 patients, who had been...
Background Osmotic Response Element-Binding Protein (OREBP), also known as TonEBP or NFAT5, is a unique transcription factor. It hitherto the only mammalian factor that regulates hypertonic stress-induced gene transcription. In addition, unlike other monomeric members of NFAT family, OREBP exists homodimer and it to bind naked DNA targets by complete encirclement in vitro. Nevertheless, how interacts with target DNA, ORE/TonE, elicits vivo, remains unknown. Methodology Using induction aldose...
Hepatitis B virus (HBV) is an important cause of human chronic liver diseases and a major public health problem. Viral load HBV genotype play critical roles in determining clinical outcomes response to antiviral treatment hepatitis patients. detection quantification assays are currently use with different levels effectiveness. In this study, the performance real-time genotyping quantitative PCR (GQ-PCR)-based assay was evaluated. Through genotype-specific primers probes, provides...
Abstract Phenotypic assays of hepatitis B virus (HBV) play an important role in research related to the problem drug resistance that emerges during long‐term nucleot(s)ide therapy patients with chronic B. Most phenotypic assay systems are available currently rely on transfection recombinant replication‐competent HBV DNA into hepatoma cell lines. Cloning clinical isolates using conventional digestion‐and‐ligation techniques generate recombinants can be very difficult because sequence...