A5080375606- Neuroscience and Neuropharmacology Research
- Neurotransmitter Receptor Influence on Behavior
- Receptor Mechanisms and Signaling
- Cannabis and Cannabinoid Research
- Memory and Neural Mechanisms
- Neurological disorders and treatments
- Ion channel regulation and function
- Neuropeptides and Animal Physiology
- Neural dynamics and brain function
- Neuroendocrine regulation and behavior
- Parkinson's Disease Mechanisms and Treatments
- Nicotinic Acetylcholine Receptors Study
- Adipose Tissue and Metabolism
- Neuroinflammation and Neurodegeneration Mechanisms
- Circadian rhythm and melatonin
- Genetics and Neurodevelopmental Disorders
- Sleep and Wakefulness Research
- Neuroscience of respiration and sleep
- Pain Mechanisms and Treatments
- Regulation of Appetite and Obesity
- EEG and Brain-Computer Interfaces
- GABA and Rice Research
- Psychedelics and Drug Studies
- Genetic Neurodegenerative Diseases
- Neurobiology and Insect Physiology Research
Inserm
2016-2025
Centre National de la Recherche Scientifique
2016-2025
Université de Montpellier
2015-2024
Institut de Génomique Fonctionnelle
2015-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2023
Institute for Neurosciences of Montpellier
2016-2023
Harvard University
2023
Massachusetts General Hospital
2023
Université de Bordeaux
2023
Johns Hopkins University
2023
Many drugs of abuse exert their addictive effects by increasing extracellular dopamine in the nucleus accumbens, where they likely alter plasticity corticostriatal glutamatergic transmission. This mechanism implies key molecular alterations neurons which both and glutamate inputs are activated. Extracellular signal-regulated kinase (ERK), an enzyme important for long-term synaptic plasticity, is a good candidate playing such role. Here, we show mouse that d -amphetamine activates ERK subset...
Psychostimulants and other drugs of abuse activate extracellular signal-regulated kinase (ERK) in the striatum, through combined stimulation dopamine D 1 receptors (D1Rs) glutamate NMDA receptors. Antipsychotic similar signaling proteins striatum by blocking 2 (D2Rs). However, neurons which these pathways are activated psychotropic not precisely identified. We used transgenic mice, enhanced green fluorescent protein (EGFP) expression was driven D1R promoter ( drd1a -EGFP) or D2R drd2 -EGFP)....
A central feature of drugs abuse is to induce gene expression in discrete brain structures that are critically involved behavioral responses related addictive processes. Although extracellular signal-regulated kinase (ERK) has been implicated several neurobiological processes, including neuronal plasticity, its role drug addiction remains poorly understood. This study was designed analyze the activation ERK by cocaine, involvement cocaine-induced early and long-term effects, as well...
Abstract A major goal of research on addiction is to identify the molecular mechanisms long‐lasting behavioural alterations induced by drugs abuse. Cocaine and delta‐9‐tetrahydrocannabinol (THC) activate extracellular signal‐regulated kinase (ERK) in striatum blockade ERK pathway prevents establishment conditioned place preference these drugs. However, it not known whether activation specific for two and/or this brain region. We studied appearance phospho‐ERK immunoreactive neurons CD−1...
The molecular basis of l-3,4-dihydroxyphenylalanine (l-DOPA)-induced dyskinesia (LID), one the major hindrances in current therapy for Parkinson9s disease, is still unclear. We show that attenuation cAMP signaling medium spiny neurons striatum, achieved by genetic inactivation dopamine and cAMP-regulated phosphoprotein 32 kDa (DARPP-32), reduces LID. also that, dyskinetic mice, sensitized cAMP/cAMP-dependent protein kinase/DARPP-32 leads to phosphorylation/activation extracellular...
The direct and indirect pathways of the basal ganglia have been proposed to oppositely regulate locomotion differentially contribute pathological behaviors. Analysis distinct contributions each pathway behavior has a challenge, however, due difficulty selectively investigating neurons comprising two using conventional techniques. Here we present mouse models in which function striatonigral or striatopallidal is disrupted cell type–specific deletion striatal signaling protein dopamine-...
Abstract Fentanyl is a powerful painkiller that elicits euphoria and positive reinforcement 1 . also leads to dependence, defined by the aversive withdrawal syndrome, which fuels negative 2,3 (that is, individuals retake drug avoid withdrawal). Positive maintain opioid consumption, addiction in one-fourth of users, largest fraction for all addictive drugs 4 Among receptors, µ-opioid receptors have key role 5 , yet induction loci circuit adaptations eventually lead remain unknown. Here we...
Endocannabinoids form a novel class of intercellular messengers, the functions which include retrograde signaling in brain and mediation or modulation several types synaptic plasticity. Yet, mechanisms long-term effects stimulation CB1 cannabinoid receptors (CB1-R) are poorly understood. We show that anandamide, 2-arachidonoyl-glycerol, Δ9-tetrahydrocannabinol (THC) activated extracellular signal-regulated kinase (ERK) hippocampal slices. In living mice, THC ERK neurons induced its...
Behavioural and pharmacological effects of Δ9‐tetrahydrocannabinol (THC) nicotine are well known. However, the possible interactions between these two drugs abuse remain unclear in spite current association cannabis tobacco humans. The present study was designed to analyse consequences administration on THC‐induced acute behavioural biochemical responses, tolerance physical dependence. Nicotine strongly facilitated hypothermia, antinociception hypolocomotion induced by THC. Furthermore,...
Repeated association of drugs abuse with context leads to long-lasting behavioral responses that reflect reward-controlled learning and participate in the establishment addiction. Reactivation consolidated memories is known produce a reconsolidation process during which undergo labile state. We investigated whether reexposure had similar effects. Cocaine administration activates extracellular signal-regulated kinase (ERK) striatum, ERK activation required for acquisition cocaine-induced...
Although the induction of persistent behavioral alterations by drugs abuse requires regulation gene transcription, precise intracellular signaling pathways that are involved remain mainly unknown. Extracellular signal-regulated kinase (ERK) is critical for expression immediate-early genes in striatum response to cocaine and Δ9-tetrahydrocannabinol rewarding properties these drugs. Here we show mice a single injection (10 mg/kg) activates mitogen- stress-activated protein 1 (MSK1) dorsal...
Precise identification of neuronal populations is a major challenge in neuroscience. In the striatum, more than 95% neurons are GABAergic medium-sized spiny (MSNs), which form two intermingled distinguished by their projections and protein content. Those expressing dopamine D(1)-receptors (D1Rs) project preferentially to substantia nigra pars reticulata (SNr), whereas those D(2)- receptors (D2Rs) lateral part globus pallidus (LGP). The degree segregation these has been continuous subject...
Dyskinetic side effects of a Parkinson’s disease medication may involve dopamine D1 receptor–mediated activation mTORC1.
Akt is classically described as a prosurvival serine/threonine kinase activated in response to trophic factors. After activation by phosphoinositide 3-kinase (PI3-kinase), it can translocate the nucleus where promotes specific genetic programs catalyzing phosphorylation of transcription We report here that both dopamine (DA) D1 (SKF38393) and D2 (quinpirole) agonist treatments rapidly increase, primary striatal neurons culture, levels on Thr(308), residue critically involved its activity....
Tolerance and dependence induced by chronic Δ‐9‐tetrahydrocannabinol (THC) administration were investigated in mice. The effects on body weight, analgesia hypothermia measured during 6 days of treatment (10 or 20 mg kg −1 THC twice daily). A rapid tolerance to the acute was observed from second administration. selective CB‐1 receptor antagonist SR 141716A ) administered at end treatment, somatic vegetative manifestations abstinence evaluated. precipitated several signs that included wet dog...
In the dopamine-depleted striatum, extracellular signal-regulated kinase (ERK) signaling is implicated in development of L-DOPA-induced dyskinesia. To gain insights on its role this disorder, we examined effects L-DOPA state phosphorylation ERK and downstream target proteins striatopallidal striatonigral medium spiny neurons (MSNs). For purpose, employed mice expressing enhanced green fluorescent protein (EGFP) under control promoters for dopamine D(2) receptor (Drd2-EGFP mice) or D(1)...
Abstract Background Repeated exposure to psychostimulants results in a progressive and long-lasting facilitation of the locomotor response that is thought have implications for addiction. Psychostimulants other drugs abuse activate specific brain areas extracellular signal-regulated kinase (ERK), an essential component signaling pathway involved synaptic plasticity long-term effects abuse. Here we investigated role ERK activation behavioral sensitization induced by repeated administration...