A5042533344- T-cell and Retrovirus Studies
- Toxoplasma gondii Research Studies
- Vector-Borne Animal Diseases
- Retinoids in leukemia and cellular processes
- Acute Myeloid Leukemia Research
- Animal Disease Management and Epidemiology
- Histone Deacetylase Inhibitors Research
- Cytomegalovirus and herpesvirus research
- Herpesvirus Infections and Treatments
- Viral-associated cancers and disorders
- Cancer therapeutics and mechanisms
- Parasitic Infections and Diagnostics
- Lymphoma Diagnosis and Treatment
- Research on Leishmaniasis Studies
- Rabies epidemiology and control
- Autophagy in Disease and Therapy
- Synthesis and Biological Evaluation
- Photodynamic Therapy Research Studies
- CNS Lymphoma Diagnosis and Treatment
- Heme Oxygenase-1 and Carbon Monoxide
- RNA Interference and Gene Delivery
- Ubiquitin and proteasome pathways
- Mosquito-borne diseases and control
- Mesenchymal stem cell research
- Protein Degradation and Inhibitors
American University of Beirut
2016-2025
American University of Beirut Medical Center
2014-2020
Université Paris Cité
2020
Hôpital Necker-Enfants Malades
2020
American University of Science and Technology
2020
Inserm
2020
Assistance Publique – Hôpitaux de Paris
2020
Lebanese University
2009
Sheikh Ragheb Harb Hospital
2009
Université de Montpellier
2005-2008
Toxoplasma gondii strains differ dramatically in virulence despite being genetically very similar. Genetic mapping revealed two closely adjacent quantitative trait loci on parasite chromosome VIIa that control the extreme of type I lineage. Positional cloning identified candidate gene ROP18 , a highly polymorphic serine-threonine kinase was secreted into host cell during invasion. Transfection virulent allele nonpathogenic III strain increased growth and enhanced mortality by 4 to 5 logs....
Host cell invasion in the Apicomplexa is unique its dependency on a parasite actin-driven machinery and exclusion of most host membrane proteins during parasitophorous vacuole (PV) formation. This occurs at junction between plasma membranes that has been called moving junction, circumferential zone which forms apical tip parasite, moves backward eventually pinches PV from membrane. Despite having described by electron microscopic studies 30 years ago, molecular nature this singular structure...
Toxoplasma gondii is an obligate intracellular parasite for which the discharge of apical organelles named rhoptries a key event in host cell invasion. Among rhoptry proteins, ROP2, prototype large protein family, translocated parasitophorous vacuole membrane during The ROP2 family members are related to protein-kinases, but only some them predicted be catalytically active, and none latter has been characterized so far. We show here that ROP18, member located re-localises at demonstrate...
Four rhoptry proteins (ROP) of Toxoplasma gondii previously identified with mAb have been affinity purified and analyzed by MS; the data obtained allowed genomic sequences to be assigned these proteins. As suggested for some them antibody crossreactivity, were shown belong a family, prototype which being ROP2. We describe here ROP2, 4, 5, 7. These four correspond most abundant products gene family that comprises several members we in EST libraries. Eight additional found cloned them. All...
Chronic HTLV-I (human T cell lymphotropic virus type I) infection may cause adult leukemia/lymphoma (ATL), a disease with dismal long-term prognosis. The transactivator, Tax, initiates ATL in transgenic mice. In this study, we demonstrate that an As2O3 and IFN-α combination, known to trigger Tax proteolysis, cures Tax-driven Unexpectedly, combination therapy abrogated initial leukemia engraftment into secondary recipients, whereas the primary tumor bulk still grew hosts, only ultimately...
Abstract Acute myeloid leukemia (AML) pathogenesis often involves a mutation in the NPM1 nucleolar chaperone, but bases for its transforming properties and overall association with favorable therapeutic responses remain incompletely understood. Here we demonstrate that an oncogenic mutant form of (NPM1c) impairs mitochondrial function. NPM1c also hampers formation promyelocytic (PML) nuclear bodies (NB), which are regulators fitness key senescence effectors. Actinomycin D (ActD), antibiotic...
A comparison was made between DNA fingerprints of Mycobacterium tuberculosis produced with the insertion sequence IS6110 and those polymorphic GC-rich repetitive contained in plasmid pTBN12. total 302 M. isolates from prison system Madrid, Spain, Denver Public Health Department (Denver, Colo.) were analyzed two probes. Both probes identified same clusters when had six or more copies IS6110. Analysis unique demonstrated that they The pTBN12 probe greater discriminating power having five fewer...
Toxoplasma gondii, as many intracellular parasites, is separated from the cytosol of its host cell by a parasitophorous vacuole membrane (PVM). This forms during invasion and parasite apical organelles named rhoptries discharge proteins that associate with this process. We report here characterization rhoptry protein ROP5, which new member ROP2 family. Contrasting what known for other family proteins, ROP5 not processed trafficking to rhoptries. show secreted associates PVM. Using...
The human T-lymphotropic virus type I oncoprotein Tax is critical for T-cell transformation, acting mainly through nuclear factor kappa B essential modulator (NEMO) binding and subsequent factor-κB activation. localizes to bodies the centrosome subjected ubiquitylation small ubiquitin-like modifier (SUMO)ylation, which are both necessary complete transcriptional Using photoconvertible fluorophore Dendra-2 coupled with live video confocal microscopy, we show first time that same molecule...
Aurora kinases are eukaryotic serine/threonine protein that regulate key events associated with chromatin condensation, centrosome and spindle function cytokinesis. Elucidating the roles of in apicomplexan parasites is crucial to understand cell cycle control during Plasmodium schizogony or Toxoplasma endodyogeny. Here, we report on localization two previously uncharacterized Aurora-related (Ark2 Ark3) tachyzoites Ark3 orthologue falciparum erythrocytic stages. In gondii, show TgArk2 TgArk3...
The protozoan parasite Toxoplasma gondii is equipped with a sophisticated secretory apparatus, including three distinct exocytic organelles, named micronemes, rhoptries, and dense granules. We have dissected the requirements for targeting microneme protein MIC3, key component of T. infection. shown that MIC3 processed in post-Golgi compartment propeptide epidermal growth factor (EGF) modules contain microneme-targeting information. minimal requirement delivery defined by plus any one EGF...
Abstract Background HTLV-I associated adult T-cell leukemia/lymphoma (ATL) carries a dismal prognosis due to chemo-resistance and immuno-compromised micro-environment. The combination of zidovudine interferon-alpha (IFN) significantly improved survival in ATL. Promising results were reported by adding arsenic trioxide IFN. Results Here we assessed Th1/Th2/T reg cytokine gene expression profiles 16 ATL patients before 30 days after treatment with arsenic/IFN/zidovudine, comparison healthy...
Retroviruses are associated with a wide variety of diseases, including immunological, neurological disorders, and different forms cancer. Among retroviruses, Oncovirinae regroup according to their genetic structure sequence, several related viruses such as human T-cell lymphotropic types 1 2 (HTLV-1 HTLV-2), simian T cell (STLV-1 STLV-2), bovine leukemia virus (BLV). As in many animal models provide useful tool for the studies pathogenesis, treatment, prevention. In current review, an...
Primary effusion lymphoma (PEL) is a rare aggressive subset of non-Hodgkin B-cell lymphoma. It caused by Kaposi sarcoma-associated herpesvirus/human herpesvirus type 8 (KSHV/HHV8). occurs mainly, but not exclusively, in HIV-positive patients. PEL predominantly develops serous cavities and occasionally extracavitary regions. carries very poor prognosis with median survival time <6 months. Indeed, currently used treatment modalities such as CHOP chemotherapy are far from achieving complete...