A5103134907- Cancer Research and Treatments
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Ubiquitin and proteasome pathways
- Occupational and environmental lung diseases
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Enzyme Production and Characterization
- Salivary Gland Disorders and Functions
- Pancreatic and Hepatic Oncology Research
- Oral microbiology and periodontitis research
- Glycosylation and Glycoproteins Research
- Cancer, Lipids, and Metabolism
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Caveolin-1 and cellular processes
- Endoplasmic Reticulum Stress and Disease
- Peptidase Inhibition and Analysis
- Trace Elements in Health
- Thyroid Disorders and Treatments
- Cancer, Hypoxia, and Metabolism
- Microbial Metabolites in Food Biotechnology
- Heavy Metal Exposure and Toxicity
- Tissue Engineering and Regenerative Medicine
- Circular RNAs in diseases
- Extracellular vesicles in disease
- Liver physiology and pathology
Wuhan University
2021-2025
Zhongnan Hospital of Wuhan University
2021-2025
Wuhan Prevention and Treatment Center for Occupational Diseases
2025
Zhengzhou University
2022-2024
Fujian Academy of Agricultural Sciences
2017
University of Louisville
2010-2016
Hebei North University
2007
<h3>Background</h3> Rheumatoid arthritis (RA) is characterised by autoimmunity to citrullinated proteins, and there increasing epidemiologic evidence linking <i>Porphyromonas gingivalis</i> RA. <i>P apparently unique among periodontal pathogens in possessing a citrullinating enzyme, peptidylarginine deiminase (PPAD) with the potential generate antigens driving autoimmune response. <h3>Objectives</h3> To examine immune response PPAD patients RA, individuals periodontitis (PD) controls...
Abstract Porphyromonas gingivalis is a member of the human oral microbiome abundant in dysbiosis and implicated pathogenesis periodontal (gum) disease. It employs newly described type-IX secretion system (T9SS) for virulence factors. Cargo proteins destined through T9SS carry recognition signal conserved C-terminal domain (CTD), which removed by sortase PorU during translocation. Here, we identified novel component T9SS, PorZ, essential surface exposure posttranslational modification cargo...
With the highest cancer incidence rate, lung cancer, especially non-small cell (NSCLC), is leading cause of death in world. Tea (leaves Camellia sinensis) has been widely used as a traditional beverage beneficial to human health, including anti-NSCLC activity. Theabrownin (TB) one major kind tea pigment responsible for effects liquor. However, its effect on NSCLC unknown. The aim present study was evaluate anti-proliferative and apoptosis-inducing TB (A549) cells, using MTT assay,...
Green tea, the fresh leaves of Camellia sinensis, is not only a health-promoting beverage but also traditional Chinese medicine used for prevention or treatment cancer, such as lung cancer. Theabrownin (TB) main fraction responsible medicinal effects green whether it possesses anti-cancer effect unknown yet. This study aimed to determine in vitro and vivo anti-lung cancer TB explore underlying molecular mechanism, by using A549 cell line Lewis carcinoma-bearing mice. In cellular experiment,...
Abstract Serum deprivation-response protein (SDPR), a phosphatidylserine-binding protein, which is known to have promising role in caveolar biogenesis and morphology. However, its function hepatocellular carcinoma (HCC) was still largely unknown. In this study, we discussed the characterization identification of SDPR, present it as novel apoptosis candidate incidence HCC. We identified 81 HCC cases with lower SDPR expression tumor tissues help qRT-PCR assay, potentially associated poor...
High mobility group protein 1 (HMGB1) is a highly conserved DNA-binding nuclear that participates in the occurrence and development of silicosis. HMGB1 binds to its specific receptor activates phosphatidylinositol 3-kinase (PI3K)/protein kinase B, (PKB; Akt)/mammalian target rapamycin (mTOR) pathway. Brahma-related genes (BRG1; SMARCA4) core subunit SWI/SNF. Akt pathway through BRG1 promote proliferation prostate cancer. Glycyrrhizic acid new pharmacological inhibitor HMGB1, which may...
Metabolic reprogramming, particularly the Warburg effect, plays a crucial role in onset and progression of tumors. The ubiquitin-conjugating enzyme E2 Q2 (UBE2Q2) has been identified overexpressed hepatocellular carcinoma (HCC). Our aim was to determine if UBE2Q2 regulating glycolysis, contributing carcinogenesis HCC. Bioinformatics analysis, western blot qPCR were used detect expression UBE2Q2. Functional experiments, proteomics analysis subcutaneous tumors constructed find biological...
Gingipain proteases are important virulence factors from the periodontal pathogen Porphyromonas gingivalis and target of many in vitro studies. Due to their close biochemical properties, purification individual gingipains is difficult requires multiple chromatographic steps. In this study, we demonstrate that insertion a hexahistidine affinity tag upstream C-terminal outer membrane translocation signal RgpB gingipain leads secretion soluble, mature form bearing can easily be purified by...
Abstract Despite of advances in treatment options, hepatocellular carcinoma (HCC) remains nearly incurable and has been recognized as the third leading cause cancer-related deaths worldwide. As a deubiquitinating enzyme, antitumor effect ubiquitin-specific peptidase 53 (USP53) demonstrated on few malignancies. In this study, we investigated potential role USP53 HCC. The results showed that was downregulated HCC tissues well cell lines using both silico data patient samples. Furthermore,...
Silicosis, a disease characterized by diffuse fibrosis of the lung tissue, is caused long-term inhalation free silica (SiO
Silicosis is an incurable lung disease that can progress even when exposure to silica dust has ended. Lipid metabolism plays important role in the occurrence and development of silicosis. However, mechanistic details have not been fully elucidated. This was investigated current study by high-performance liquid chromatography-mass spectrometry-based lipidomic analysis tissue a mouse model profiles key metabolic enzymes were compared between control groups. The revealed...
Introduction: Hepatocellular carcinoma (HCC) was the sixth most prevalent cancer worldwide. Long non-coding RNA TGFB2-OT1 has been proven to mediate inflammation and autophagy in vascular endothelial cells. However, its function HCC is still unknown. Methods: We analyzed relationship between expression clinicopathological features of 202 patients. RT-qPCR used analyze cell lines tissues. In vitro vivo assays were conducted verify effect on phenotype HCC. pull-down applied reveal proteins...
Cyclodextrin glycosyltransferase (CGTase) is an important industrial enzyme for production of cyclodextrins (CDs) from starch by intramolecular transglycosylation. CGTase consists five domains labeled A to E. For optimizing catalytic activity CGTase, Geobacillus sp. was fused with the family 20 carbohydrate-binding module (CBM) Bacillus circulans strain 251 CGTase. The CBMbc251 that has a low binding free energy maltohexaose, selected in silico design. Then fusion enzyme, CGTΔE-CBMbc251,...
Abstract Hepatocellular carcinoma (HCC) is a leading cause of cancer death worldwide, accounting for over 700,000 deaths each year. The lack predictive and prognostic biomarkers HCC, with effective therapy, remains significant challenge HCC management. Long non-coding RNAs (lncRNAs) play key role in tumorigenesis have clinical value as potential the early diagnosis prediction HCC. Jun activation domain-binding protein 1 (Jab1, also known COP9 signalosome subunit 5, CSN5) oncogene that plays...
Background: Excessive accumulation of extracellular matrix is a key feature pulmonary fibrosis (PF), and myofibroblasts are the main producers matrix. Fibroblasts major source myofibroblasts, but mechanisms transdifferentiation unclear. Methods: In vitro, transforming growth factor-β1 was used to induce NIH-3T3 cell transdifferentiation. DMOG increase hypoxia-inducible factor-1α subunit (HIF-1α) expression. KC7F2 siRNA decreased HIF-1α vivo, silica particles were PF in C57BL/6N mice, reduce...
Cavin1 is a cell membrane caveolin, with controversial function in different tumors. Meanwhile, the role of hepatocellular carcinoma (HCC) progression remains unclear. In this study, we attempted to elucidate significance HCC occurrence and progression. content was examined tissues paired adjacent normal liver by qRT-PCR IHC among 81 patients. The Cavin1-mediated regulation proliferation metastasis assessed through vitro vivo experiments. Finally, using GSEA, found out could be potential...
Manganese is essential trace elements, to participate in the body a variety of biochemical reactions, has important physiological functions, such as stimulate immune cell proliferation, strengthen cellular immunity, etc. However, excessive manganese exposure can cause damage multiple systems body.The system extremely vulnerable external toxicants, however research on are inadequate and biomarkers lacking. Therefore, here we applied manganese-exposed rat model make preliminary observations...
Intrahepatic cholangiocarcinoma (ICC) is one of the most commonly diagnosed malignancies worldwide, and second common primary liver tumor. The lack effective diagnostic treatment methods results in poor patient prognosis high mortality rate. Atypical protein kinase C-ι (aPKC-ι) highly expressed metastatic ICC tissues, regulates epithelial mesenchymal transition (EMT) through aPKC-ι/P-Sp1/Snail signaling pathway. Recent studies have correlated aberrant glucose metabolism with EMT. Given vital...