A5101811865- Oral microbiology and periodontitis research
- Dental Health and Care Utilization
- Endodontics and Root Canal Treatments
- Dental Research and COVID-19
- Streptococcal Infections and Treatments
- Bacterial biofilms and quorum sensing
- Dental Anxiety and Anesthesia Techniques
- Regulation of Appetite and Obesity
- Adipokines, Inflammation, and Metabolic Diseases
- Biochemical Analysis and Sensing Techniques
- Intramuscular injections and effects
- Biochemical and Structural Characterization
- Dental Erosion and Treatment
- Fluoride Effects and Removal
- Data Quality and Management
- Dental Trauma and Treatments
- Bone Tissue Engineering Materials
- Salivary Gland Disorders and Functions
- Inflammasome and immune disorders
- Child Nutrition and Water Access
- Facial Trauma and Fracture Management
- Dental Radiography and Imaging
- Machine Learning in Bioinformatics
- Listeria monocytogenes in Food Safety
- Inflammatory mediators and NSAID effects
Shanghai Ocean University
2025
The University of Sydney
2013-2023
Westmead Hospital
2013-2023
Westmead Institute
2013-2020
Bridge University
2020
Sydney Dental Hospital
2019
Westmead Institute for Medical Research
2017
Millennium Institute
2013
Abstract Porphyromonas gingivalis is a member of the human oral microbiome abundant in dysbiosis and implicated pathogenesis periodontal (gum) disease. It employs newly described type-IX secretion system (T9SS) for virulence factors. Cargo proteins destined through T9SS carry recognition signal conserved C-terminal domain (CTD), which removed by sortase PorU during translocation. Here, we identified novel component T9SS, PorZ, essential surface exposure posttranslational modification cargo...
Abstract Porphyromonas gingivalis is a keystone bacterial pathogen of chronic periodontitis. P . unable to synthesise the porphyrin macrocycle and relies on exogenous porphyrin, including haem or biosynthesis intermediates from host sources. We show that under iron-limited conditions prevailing in tissue environments, expresses haemophore-like protein, HusA, mediate uptake essential support survival within epithelial cells. The structure together with titration studies, mutagenesis silico...
Abstract Next Generation sequencing has greatly progressed the exploration of oral microbiome’s role in dental diseases, however, there been little focus on effect sample storage conditions and their interaction with DNA extraction method. Dental plaque samples collected from 20 healthy participants were pooled stored either 75% ethanol or Bead solution for up to 6-months at −80 °C, prior QIAamp (non-bead beating) PowerSoil (bead-beating) kit, followed by Illumina 16S rRNA gene . We found...
The Gram-negative periodontal pathogen Porphyromonas gingivalis produces a family of outer membrane-anchored proteases, the gingipains, shown to play an essential role in virulence organism. C-terminal domain (CTD) gingipains and other secreted proteins is known be targeting signal for maturation translocation protein through membrane. CTD subsequently cleaved during secretion process. Multiple alignment various CTDs failed define consensus sequence at putative processing site. Using...
Vibrio parahaemolyticus, a halophilic Gram-negative bacterium commonly found in aquatic products, can colonize the human small intestine, causing gastroenteritis and potentially leukemia. As major intestinal pathogen, it poses significant threat to public health. This study aims investigate phenotypic heterogeneity of V. parahaemolyticus low-salinity bile salt environments tract elucidate its mechanisms tolerance pathogenicity using proteomics. The experimental results indicated that under...
Background The aim of this study was to determine the caries removal effectiveness (CRE) and minimal invasiveness potential (MIP) four dentine methods. Methods After carious molars were scanned using micro-computerized tomography (micro-CT), removed by fluorescence aided excavation (FACE) technology, laser induced (LIF), chemomechanical (CME), conventional (CE). Micro-CT then repeated. CRE determined based on volume residual caries/initial (RC/IC) mean mineral density (MD) at cavity floor....
Introduction: Increasing attention is being given to the roles of data management and sharing in advancement research. This study was undertaken explore opinions past experiences established dental researchers as related management. Methods: Researchers were recruited from International Association for Dental Research scientific groups complete a survey consisting Likert-type, multiple-choice, open-ended questions. Results: All 42 respondents indicated that should be promoted facilitated,...
Abstract Background Recent preventive strategies for dental caries focus on targeting the mechanisms underlying biofilm formation, including inhibition of bacterial adhesion. A promising approach to prevent adhesion is modify composition acquired salivary pellicle. This in vitro study investigated effect and possible mechanism pellicle modification by casein phosphopeptide (CPP) Streptococcus mutans ( S. ) initial adhesion, impact fluoride efficacy CPP. Methods The pellicle-coated...
Ghrelin plays a major role in obesity-related diseases which have been shown to be associated with periodontitis. This study sought analyze the expression of functional receptor for ghrelin (GHS-R1a) periodontal cells and tissues under microbial conditions vitro vivo. The GHS-R1a human challenged periodontopathogen Fusobacterium nucleatum, gingival biopsies from periodontally healthy diseased individuals, rats without ligature-induced periodontitis was analyzed by real-time PCR,...
Aging population will lead to the increase of incidence root caries globally. The clinical management is challenging due difficulty in moisture isolation. caused by release organic acids from cariogenic bacteria which results dissolution cementum and dentin root. purpose this study efficacy modified saturated calcium phosphate solution (CaP) supplement with zinc (Zn(2+)) and/or fluoride (F(-)) providing surfaces less susceptible acid bacterial colonization.Human sections extracted premolars...
Abstract Background The COVID-19 pandemic challenged all healthcare providers including dental practitioners. This cross-sectional study aimed to investigate the practitioners’ perceptions and attitudes towards impacts of on their professional practice, career decision patient care. Methods Data was collected from practitioners registered in New South Wales (NSW), Australia using an online survey. Results conclusion Responses received 206 revealed infection risk, clinical guidelines,...
Abstract Background: Recent preventive strategies for dental caries focus on targeting the mechanisms underlying biofilm formation, including inhibiting bacterial adhesion. A promising approach to prevent adhesion is modify composition of acquired salivary pellicle. This in vitro study investigated effect and possible mechanism pellicle modification by casein phosphopeptide (CPP) Streptococcus mutans ( S. ) initial adhesion, impact fluoride efficacy CPP. Methods: The pellicle-coated...