A5016928831- Immune Cell Function and Interaction
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Cytokine Signaling Pathways and Interactions
- Mesenchymal stem cell research
- Cancer Immunotherapy and Biomarkers
- Immune Response and Inflammation
- Systemic Lupus Erythematosus Research
- Bone and Joint Diseases
- Lipid metabolism and disorders
- Animal health and immunology
- Viral Infectious Diseases and Gene Expression in Insects
- NF-κB Signaling Pathways
- Cell Adhesion Molecules Research
- Hematological disorders and diagnostics
- Immune cells in cancer
- Hip disorders and treatments
- RNA Interference and Gene Delivery
- Aquaculture Nutrition and Growth
- T-cell and Retrovirus Studies
- Liver physiology and pathology
- Hedgehog Signaling Pathway Studies
- Invertebrate Immune Response Mechanisms
Zhejiang Chinese Medical University
2018-2023
University of Louisville
2019-2023
Henan Normal University
2023
Federation of American Societies for Experimental Biology
2020
National Institutes of Health
2020
Yale University
2020
Harvard University
2020
Rockefeller University
2020
Brigham and Women's Hospital
2020
Cornell University
2020
Despite the remarkable success of immunotherapy in many types cancer, pancreatic ductal adenocarcinoma has yet to benefit. Innate immune cells are critical anti-tumor immunosurveillance and recent studies have revealed that these populations possess a form memory, termed trained innate immunity, which occurs through transcriptomic, epigenetic, metabolic reprograming. Here we demonstrate yeast-derived particulate β-glucan, an inducer traffics pancreas, causes CCR2-dependent influx...
Regulation of myeloid-derived suppressor cells (MDSC) by ongoing inflammation following repeated chemotherapy remain elusive. In this study, we show that a multidose clinical regimen gemcitabine (GEM) treatment enhances the immunosuppressive function monocytic MDSC (M-MDSC), although tumor development is delayed in E0771 tumor-bearing mice. Accordingly, effector IFN-γ-producing CD4 and CD8 T are significantly decreased microenvironment (TME) GEM-treated The conditioned medium differentiation...
Mesenchymal stem cells (MSCs) play a crucial role in tissue repair by secretion of nutrient factors such as hepatocyte growth factor (HGF). However, studies examining the effects HGF on proliferation and differentiation MSCs used different concentrations reported conflicting conclusions. This study aimed to determine mechanisms which regulate MSC osteogenic differentiation, validate mechanism an animal model early stage avascular necrosis femoral head (ANFH). Our results demonstrate that low...
Background The importance of CD4+ and CD8+ T cells in protection against tuberculosis (TB) is well known, however, the association between changes to cell repertoire disease presentation has never been analyzed. Characterization T-cells TB patients previous study only analyzed TCR β chain omitted analysis Vα family even though α also contribute antigen recognition. Furthermore, limited information available regarding heterogeneity compartment overall function as common structural features...
Autologous transplantation of modified mesenchymal stem cells (MSCs) is a promising candidate for the treatment refractory clinical disease, avascular necrosis femoral head (ANFH). Our previous attempts by compounding MSCs with medical fibrin glue to treat ANFH in animal model have achieved excellent effects. However, underlying molecular mechanism unclear, especially on transgenic gene expression. Rabbit were isolated and compounded glue. Following degrading glue, proliferation, viability,...
To investigate the correlation between normalization of T cell receptor (TCR) repertoire and remission advanced colorectal cancer. Forty-one patients were randomly assigned to receive either folinic acid/fluorouracil/irinotecan alone (n = 20) or in combination with recombinant human endostatin 21). Efficacy toxicity evaluated, changes TCR diversity assessed by detecting spectratypes complementarity-determining region three before after several cycles therapy. A scoring system was used...
Potent antitumor responses can be induced through cytokine immunotherapy. Interleukin (IL)-2 and granulocyte-macrophage colony-stimulating factor (GM-CSF) are among the most effective cytokines to induce tumor-specific systemic immune act synergistically. To overcome limitations of combined use these two cytokines, we have constructed an IL2-GMCSF fusion protein characterized its effects in this study.The expression IL-2 receptor GM-CSF cell lines were detected with quantitative real-time...
The invariant natural killer T (iNKT) cell has been shown to play a central role in early stages immune responses against Mycobacterium tuberculosis (Mtb) infection, which become nonresponsive (anergic) and fails control the growth of Mtb patients with active tuberculosis. Enhancement iNKT antigens can help resist infection.In present study, an 38-kDa antigen-specific receptor (TCR) was isolated from human CD8(+) cells stimulated by antigen vitro, then transduced into primary retrovirus...
Background: Toll-like receptor 4 (TLR4) initiates both innate and adaptive immune responses, which plays an important protective role in self-defense mechanisms. Excessive or inappropriate TLR4 activation causes the development of many autoimmune diseases. Dihydropyrimidinone derivatives are medicinally molecules with diverse pharmacological activities, including anti-inflammatory activity. The present study focused on novel synthesized 3,4-dihydropyrimidinone evaluated their inhibitory...
Herein, we report (R)-3,3′-(3,5-(CF3)2-C6H3)2-BINOL-catalyzed enantioselective conjugate addition of organic boronic acids to β-silyl-α,β-unsaturated ketones, furnishing moderate excellent yields the corresponding β-silyl carbonyl compounds with stereogenic centers in enantioselectivities (up 98% ee). Moreover, catalytic system features mild reaction conditions, high efficiency, broad substrate scope, and easy scale-up.
The signal transducer and activator of transcription 3 (STAT3), which regulates multiple oncogenic processes, has been found to be constitutively activated in lymphoma, suggesting its potential as a therapeutic target. Here, we constructed an anti-CD19-N-(4-carboxycyclohexylmethyl) maleimide N-hydroxysuccinimide ester (SMCC)-protamine (CSP)-STAT3 small interfering RNA (siRNA) conjugate demonstrated that the CSP-STAT3 siRNA could specifically bind normal B cells A20 lymphoma vitro. It...
The combination of IL‐2 (interleukin‐2) and GM‐CSF (granulocyte/macrophage colony‐stimulating factor) has been broadly studied in antitumour immune therapy, but its efficacy is uncertain. To better exert the activities two cytokines study them a mouse model, we have constructed bifunctional protein, hIL‐2–mGM‐CSF (human IL‐2–mouse GM‐CSF), fused to C‐terminal tag six histidine residues (His 6 ). fusion protein was expressed Escherichia coli as IBs (inclusion bodies). After extracting...