Withania coagulans contains a complex mixture of various bioactive compounds. In order to reduce the complexity plant extract purify its phytochemical biomolecules, novel fractionation strategy using different solvent combination ratios was applied isolate twelve fractions. These fractions were tested for activity in biogenic synthesis cobalt oxide nanoparticles, biofilm and antifungal activities. The results revealed that with 30% ratio all combinations showed more potent bioreducing power,...

Silver nanoparticles have received much attention, due to their wide range of biological applications as an alternative therapy for disease conditions utilizing the nanobiotechnology domain synthesis. The current study was performed examine antioxidant, anticancer, antibacterial, and antifungal potential biosynthesized silver (TpAgNPs) using plant extract. TpAgNPs were produced by reacting Tradescantia pallida extract AgNO 3 solution in nine various concentration ratios subjected...

Present study was conducted to investigate the adsorption and ultrasound-assisted potential of silver nanoparticles (AgNPs) loaded on chitosan (AgCS composite) as nano-adsorbents for methylene blue (MB) removal. AgNPs were synthesized using leaf extract Ligustrum lucidum, which incorporated chitosan’s surface modification. UV–Vis Spectroscopy, FTIR, XRD, SEM, EDX techniques used confirm synthesis characterization nanomaterials. Batch sono-adsorption experiments removal MB executed under...

Phenol is the most common organic pollutant in many industrial wastewaters that may pose a health risk to humans due its widespread application as ingredients and additives. In this study, waste green tea leaves (WGTLs) were modified through chemical activation/carbonization used an adsorbent presence of ultrasound (cavitation) eliminate phenol aqueous solution. Different treatments, such cavitation, adsorption, sono-adsorption investigated remove phenol. The scanning electron microscope...

Soil salinity caused by NaCl is a major challenge to agricultural crops worldwide. For this, two WRKY transcription factors were evaluated for their role in salt stress tolerance tomato plants (Solanum lycopersicum; Sl). SlWRKY36 and SlWRKY51 provided novel insight into the regulatory mechanism against via virus-induced gene silencing (VIGS). Salt significantly reduced chlorophyll-a, an abundant form of chlorophyll content 6.0 5.1 mg/g proline 0.06 0.09 respectively silenced plants. This...

Wild marigold (Tagetes minuta), a flowering plant of the family Asteraceae contains compounds pharmaceutical and nutritional importance especially essential oils flavonols. Identification, characterization flavonols determination their antibacterial activity were major objectives current study. The isolation purification was accomplished using chromatographic techniques while structural elucidation completed by LC-MS NMR spectroscopy. extracts purified tested against various bacterial...

Bauhinia variegata plant is a very popular and traditionally potent ethnomedicine. Therefore, it need of hour to study ameliorative characteristics B. for novel secondary metabolites. The current was designed explore antiproliferative potential due scant reports on this aspect. Extracts various parts (flowers, leaves, bark, stem, roots) were prepared by successive maceration using organic solvents in increasing order polarity (n-hexane, ethyl acetate, methanol, water). determination...

Taxus wallichiana is an evergreen tree species found in the Himalayan region of Pakistan. The possesses important secondary metabolites such as Taxol that has been implicated treating breast, ovarian and colon cancer. Therefore keeping view importance this plant species, silver nanoparticles were synthesized using aqueous leaf extract evaluated for their anti-bacterial anti-cancer properties.Silver (Ag) (NPs) characterized optical, morphological structural features techniques UV-visible...

Abstract Dicliptera is a genus belonging to family Acanthaceae. bupleuroides small perennial herb commonly known as roxburg's foldwing. It locally used in folkloric medicines for the treatment of different diseases and general tonic wound healing. The current investigational study was optimize solvent extraction protocol, identify quantitative qualitative biochemical composition, antioxidant cytotoxic activities D. characterize gene associated with biosynthesis flavonol. plant collected from...

<abstract> <p>We investigated the combined impact of convective boundary conditions, thermal conductivity, and magnetohydrodynamic on flow a tangent hyperbolic nanofluid across stratified surface. Furthermore, ramifications Brownian motion, thermophoresis, activation energy were considered. Heat generation, chemical reactions, mixed convection, other elements considered when analyzing heat transfer phenomena. The governing equations converted via similarity transformations into...

Introduction: This study aims to isolate and characterize potential cytotoxic compounds from the roots of Bauhinia variegata Linn. (Caesalpiniaceae) evaluate their activity against human cancer cell lines. Five compounds, namely β-sitosterol (1), piperine (2), piperolein B (3), retrofractamide A (4), dehydropipernonaline (5), were isolated B. using various chromatographic procedures. Methods: The root extracts prepared aqueous organic solvents, including n-hexane, ethyl acetate, methanol....

Wild marigold (Tagetes minuta L.) is grown over a wider range of climatic conditions starting from 3000 to 11000 feet altitude in the north and Northwestern parts Pakistan. The plant yield strongly aromatic essential oils other compounds, reported be great medicinal importance. information regarding biological biochemical activities compounds present Tagetes Northern Pakistan unknown. In current study our main goal was carry out bioassays bioactive extracts seeds naturally growing were...

In the present study ultrasound (US) and ultrasound-assisted hydrogen peroxide, iron sulfate mediated Fenton process (sono-Fenton) was carried out for degradation of Congo red (CR) dye.Degradation rate, COD removal, mechanism dye in combined were studied factors affecting system performance i.e., pH, initial concentration, ultrasonic power, time, effect variations concentrations peroxide also investigated.The results showed that removal sono-Fenton 83% 89% after 60 min contact time coupling...

Tagetes, a genus of flowering marigolds in the family Asteraceae (Compositeae), is reported to be medicinal plant with hypotensive, spasmolytic, anti-inflammatory, antimicrobial, and antifungal properties.Tagetes minuta characteristically contains high concentrations essential oils, flavonoids, polyphenols, polysaccharides that interfere DNA, causing erroneous or no PCR products.We tested modified various standard protocols an effort isolate high-quality DNA from different tissues T....

Nanobiotechnology is the intersection of nanotechnology and biology, where nano systems are applied to help study biological systems. There a growing interest researchers in application improving efficacy photodynamic therapy. In this study, antioxidant, photodynamic, anticancer, antibacterial potential plant extracts silver nanoparticles (AgNPs) were investigated. order synthesize AgNPs, 10 g dried powder Tecoma stans Narcissus tazetta was boiled deionized water (100 ml) mixed with aqueous...

The silver-embedded wheat straw biochar (Ag–WBC) composite was tailored effectively via the green synthetic route and used as a nano-adsorbent for removal of phenol by using adsorption sono-adsorption processes. Ligustrum lucidum leaf extract employed reducer to prepare silver nanoparticles, synthesized from pyrolysis at 450–500°C. Ag–WBC were characterized UV–Vis, SEM, EDX, FTIR. study confirms ability plant L. synthesize AgNPs composites first time. UV–vis spectroscopic analysis formation...

Potato is one of the highly consumed vegetable crop grown in different regions across Pakistan that affected by fungal diseases. The current research was conducted to identify pathogen causing mold-like disease potato Khyber Pakhtunkhwa (KP), Pakistan. For molecular identification and characterization disease; tuber samples were collected followed culturing on dextrose agar (PDA). Based morphological features, identified as a Penicillium species. This result obtained 45 isolates from tubers....

The development and optimization of controlled release lipospheres (LS) from safe biocompatible behenic acid (BA) was performed for not only enhancing patient’s compliance against highly prevailed chronic diabetes but also to vanquish the insufficiencies traditional methods drug delivery. Box-Bhenken design (BBD) utilized statistically investigate impact formulation variables on percentage yield ( <math xmlns="http://www.w3.org/1998/Math/MathML" id="M1"> <msub> <mrow> <mi>Y</mi> </mrow>...

Transgenic Arabidopsis thaliana (ecotype Columbia) was successfully transformed with the gene fructose-1,6-bisphosphatase (FBPas e) and named as AtFBPase plants. plants exhibited stable transformation, integration significantly higher expressions for gene. Morphological evaluation of transgenic showed increased plant height (35cm), number leaves (25), chlorophyll contents (28%), water use efficiency (increased from 1.5 to 2.6μmol CO2 μmol-1 H2 O) stomatal conductance (20%), which all...