A5009919146- Breast Cancer Treatment Studies
- Breast Implant and Reconstruction
- Cancer Genomics and Diagnostics
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Genomic variations and chromosomal abnormalities
- BRCA gene mutations in cancer
- Breast Lesions and Carcinomas
- RNA Research and Splicing
- Reconstructive Surgery and Microvascular Techniques
- Molecular Biology Techniques and Applications
- Genetic factors in colorectal cancer
- Cancer-related Molecular Pathways
- Circular RNAs in diseases
- Cancer Risks and Factors
- Cancer Cells and Metastasis
- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- Ubiquitin and proteasome pathways
- MicroRNA in disease regulation
- Advanced Proteomics Techniques and Applications
- Male Breast Health Studies
- Cancer and Skin Lesions
- Women's cancer prevention and management
- Reproductive Physiology in Livestock
Hospital Nossa Senhora das Graças
2015-2024
Universidade Positivo
2022
Ipsen (United Kingdom)
2022
Universidade Federal do Paraná
1999-2021
Empresa Mato-grossense de Pesquisa, Assistência e Extensão Rural
2020
Pontifícia Universidade Católica do Paraná
2018
Hospital de Clínicas
2007
Georgetown University
2006-2007
Background: Although there are many reports on different techniques in breast reconstruction, few data regarding immediate reconstruction with definitive form-stable anatomical implants terms of aesthetics and quality-of-life outcomes. Methods: Ninety-four patients underwent mastectomy using contralateral symmetrization. Aesthetic results were evaluated by three methods: the patient's self-report, assessment four independent specialists (two surgeons two plastic from institutions), BCCT.core...
Breast cancer is a very heterogeneous disease. The intrinsic molecular subtypes can explain the intertumoral heterogeneity and stem cell (CSC) hypothesis intratumoral of this kind tumor. CD44+/CD24- phenotype ALDH1 expression are major CSC markers described in invasive breast cancer. In present study, 144 samples carcinoma, no special type were distributed 15 tissue microarrays (TMA) then evaluated for to understand importance these clinical aspects classified into four according...
MicroRNAs derived from extracellular vesicles (EV-miRNAs) are circulating miRNAs considered as potential new diagnostic markers for cancer that can be easily detected in liquid biopsies. In this study, we performed RNA sequencing analysis a screening strategy to identify EV-miRNAs serum of clinically well-annotated breast (BC) patients the south Brazil. EVs three groups samples (healthy controls (CT), luminal A (LA), and triple-negative (TNBC)) were isolated using precipitation method...
Changes in the expression of protein disulfide isomerase genes PDIA3 and PDIA6 may increase endoplasmic reticulum stress, leading to cellular instability neoplasia.We evaluated invasive ductal carcinomas.Using reverse transcription-quantitative polymerase chain reaction, we compared mRNA level 45 samples carcinoma with that normal breast samples.Increased gene carcinomas (P = 0.0009) was observed.In addition, increased tumors lymph node metastasis 0.009) grade III < 0.02).The showed higher...
Abstract The R337H is a TP53 germline pathogenic variant that has been associated with several types of cancers, including breast cancer. Our main objective was to determine the frequency in sporadic cancer patients from Paraná state, South Brazil, its association prognosis and impact genomic instability. genotyping 805 tissues revealed genotypic allelic 2.36% 1.18%, respectively. In these R337H+ cases lower mean age at diagnosis observed when compared R337H-cases. Array-CGH analysis showed...
Objective: The aim of this study was to report the results prepectoral direct-to implant (DTI) reconstruction in nipple-sparing mastectomy (NSM), without acellular dermal matrices (ADM) or mesh. Methods: Multicenter cohort patients, from 2013 2020, where all sizes and types breasts, either for prophylactic therapeutic surgical purposes were included, except those with previously failed reconstruction, previous radiotherapy severe skin damage, locally advanced breast cancer, gigantomasty,...
Induction protocols based on progesterone (P4) are used prior to a synchronization program for timed-AI (TAI) increase number of pubertal heifers and pregnancy per AI (P/AI). Injectable, long-lasting P4 (iP4) is novel, practical method supplement in cattle. Here, we aimed test the effect an induction protocol single injection iP4 P/AI heifers. Bos indicus (Nellore) were classified as (PUB; n = 224) or prepubertal (PRE; 414) two ovarian ultrasonographyc exams conducted 10 d apart. Heifers...
Breast cancer (BC) is a heterogeneous disease, and establishing biomarkers essential to patient management. We previously described that extracellular vesicle-derived miRNAs (EV-miRNAs) miR-142-5p, miR-150-5p, miR-320a, miR-4433b-5p in serum discriminated BC from control samples, either alone or combined panel. Using these markers, we intend evaluate whether the same markers identified EVs are also potential tissue serum. Expression analysis using RT-qPCR was performed of 67 breast patients...
MTAP is a ubiquitously expressed gene important for adenine and methionine salvage. The located at 9p21, chromosome region often deleted in breast carcinomas, similar to CDKN2A, recognized tumor suppressor gene. Several research groups have shown that acts as suppressor, some therapeutic approaches were proposed based on tumors´ status. We analyzed CDKN2A (RT-qPCR) protein (western-blotting) expression seven cancer cell lines evaluated their promoter methylation patterns better characterize...
Triple negative breast cancer (TNBC), a clinically aggressive subtype, affects 15-35% of women from Latin America. Using an approach direct integration copy number and global miRNA profiling data, performed simultaneously in the same tumor specimens, we identified panel 17 miRNAs specifically associated with TNBC ancestrally characterized patients America, Brazil. This was differentially expressed between non-TNBC subtypes studied (p ≤ 0.05, FDR 0.25), their expression levels concordant...
To evaluate the accuracy values of 276 fine needle aspriations (FNA) breast lesions with a subsequent excisional biopsy diagnosis and to make comparison between 25 studies literature using same criteria calculate those values.Cytologic findings were compared histologic each mass. The correlation results was analyzed by decision-analysis approach, following concerning diagnostic calculated in present study other reports: sensitivity, specificity, positive predictive value, negative false...
Gene amplifications and deletions are common changes in human cancer cells. Previous studies indicate that the regions, where ACHE (7q22) BCHE (3q26.1-q26.2) genes localized, suffering such structural modifications breast cancer. Therefore, products of these genes, acetylcholinesterase butyrylcholinesterase, respectively, related to process cell differentiation proliferation, as well apoptosis. This study also included two other involved tumorigenesis, EPHB4 (7q22.1) MME (3q21-27). The aim...
Breast cancer is the second most common worldwide and first among women.Invasive ductal carcinoma (IDC) invasive lobular (ILC) are two major histological subtypes, clinical molecular differences between them justify search for new markers to distinguish them.As proteomic analysis allows a powerful analytical approach identify potential biomarkers, we performed comparative of IDC ILC samples by using two-dimensional electrophoresis mass spectrometry.Twenty-three spots were identified...
Breast cancer (BC) is the leading cause of death by this disease in women worldwide. Among factors involved tumorigenesis, long non-coding RNAs (lncRNAs) and their differential expression have been associated. Differences gene may be triggered variations DNA sequence, including single nucleotide polymorphisms (SNPs). In present study, we analyzed rs527616 (C>G), located lncRNA AQP4-AS1, using PCR-SSP 306 BC patients 312 controls, from a Brazilian population. group, frequency found for CG...
The sentinel lymph node (SLN) is considered to be the first axillary that contains malignant cells in metastatic breast tumors, and its positivity currently used clinical practice as an indication for dissection. Therefore, accurate evaluation of SLN presence essential. main aim our study characterize genomic changes present samples with ultimate goal improving predictive value evaluation. Twenty paired metastases their corresponding primary tumors (PBT) were investigated DNA copy number...