A5070887762- Veterinary Oncology Research
- Asthma and respiratory diseases
- Virus-based gene therapy research
- Infectious Diseases and Mycology
- Microbial infections and disease research
- Lymphoma Diagnosis and Treatment
- Cancer Research and Treatments
- Respiratory and Cough-Related Research
- Melanoma and MAPK Pathways
- Dermatology and Skin Diseases
- Phonocardiography and Auscultation Techniques
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Cancer-related Molecular Pathways
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Acute Myeloid Leukemia Research
- Insect symbiosis and bacterial influences
- Clinical Nutrition and Gastroenterology
- Eosinophilic Disorders and Syndromes
- Epigenetics and DNA Methylation
- Immune cells in cancer
- Cell Adhesion Molecules Research
- Cardiac Imaging and Diagnostics
- Rabbits: Nutrition, Reproduction, Health
- Immunodeficiency and Autoimmune Disorders
- Vascular Anomalies and Treatments
Tokai University
2016-2024
Tokai University Hachioji Hospital
2023-2024
North Carolina State University
2015-2024
North Central State College
2023
The University of Tokyo
2010-2018
Osaka University
2017
National Defense Medical College
2000-2012
Gunma University
1990-2009
Hitachi (Japan)
2004-2005
Tokyo Medical Center
2004
Activating mutations of the BRAF gene lead to constitutive activation MAPK pathway. Although many human cancers carry mutated gene, this mutation has not yet been characterized in canine cancers. As and share molecular abnormalities, we hypothesized that also exist To test hypothesis, sequenced exon 15 BRAF, hot spot 667 primary tumors 38 control tissues. Sequencing analysis revealed a single nucleotide T A transversion at 1349 occurred 64 (9.6%), with particularly high frequency prostatic...
Urothelial carcinoma (UC) of the lower urinary tract and prostatic (PC) are aggressive genitourinary cancers in dogs, characterized by invasion to surrounding tissues high metastatic potential. Current diagnosis canine UC PC requires histopathological examination a biopsy. Such specimens require specialized medical equipment invasive procedures, limiting availability histopathology for many patients. Access non-invasive means confirm is currently an unmet need. Recently, BRAF V595E mutation...
Abstract The aim of this study was to evaluate the relationship between breed and histopathological grade canine mast cell tumours ( MCTs ). A retrospective survey pathology data 9375 histopathologically confirmed diagnoses cutaneous in US evaluated context prevalence over two million registered purebred dogs. Association with breed, age, sex spay/neuter status assessed. indicate that proportion high‐grade increases advancing male intact dogs have increased odds developing tumours....
Background T helper‐type 2 cytokines, such as interleukin‐4 (IL‐4) and IL‐13, may play a central role in allergic diseases. The protein known ‘signal transducers activators of transcription 6’ (STAT‐6) is key factor involved both IL‐4‐ IL‐13‐mediated biological responses. Objective objective this study was to evaluate the possible STAT‐6 gene modulating atopy Japanese population. Methods We screened all 23 exons from 10 subjects for mutations by direct polymerase chain reaction (PCR)...
The Adjuvant Chemotherapy Trial of TS-1 for Colon Cancer (ACTS-CC) is a phase III trial designed to validate the non-inferiority S-1 UFT/leucovorin (LV) as postoperative adjuvant chemotherapy stage colon cancer. We report results planned safety analysis. Patients aged 20–80 years with curatively resected cancer were randomly assigned receive UFT/LV (UFT, 300 mg m−2 per day tegafur; LV, 75 on days 1–28, every 35 days, 5 courses) or (80, 100, 120 42 4 courses). Treatment status and evaluated....
Abstract: For elementary school children with atopic dermatitis, a skin care program using shower therapy was performed during the lunch break for 6 weeks from June to July in 2004 and 2005. All 53 participants showed an improvement their dermatitis 6‐week periods studied. Skin daily showering at thus found be effective treatment of dermatitis.
Abstract Recent discovery of the BRAF V595E mutation in a variety canine cancers indicates that mutant may represent novel therapeutic target. Presence RAS mutations is associated with poor tumour response to inhibition but has not been investigated ‐mutated cancers. The aim this study was evaluate mutational status three genes ( HRAS , KRAS and NRAS ) four types carcinoma without mutation. Novel were identified 18% (3/17) oral squamous cell carcinoma, whereas 17% (3/18) pulmonary carried or...
Metastasis is a multistep process in which cells must detach, migrate/invade local structures, intravasate, circulate, extravasate, and colonize. A full understanding of the complexity this has been limited by lack ability to study these steps isolation with detailed molecular analyses. Leveraging comparative oncology approach, we injected canine osteosarcoma into circulation transgenic zebrafish fluorescent blood vessels biologically dynamic metastasis extravasation model. Circulating tumor...
Macrophages play an important role in a variety of situations, including pathogen elimination, inflammation, and tissue repair. However, these cells are not fully studied dogs, part, due to the difficulty efficiently isolating culturing them vitro. In this study, we cultured canine peripheral blood mononuclear (PBMCs) with 10 ng/ml phorbol 12-myristate-13-acetate (PMA) for 5 days obtain macrophages. A high number round-adherent were obtained without addition any cytokine. These showed active...
Urothelial carcinoma (UC) is the most common neoplasm of canine urinary tract. Clinical presentation UC shared with several other, more tract disorders, and this often delays diagnosis UC. Definitive requires histopathologic examination a biopsy specimen, but cost invasiveness for these diagnostic tests result in diagnoses being made on basis clinical findings, imaging, cytologic urine sediment. Regardless process used, UCs currently are not diagnosed until they at an advanced stage so...
Breath sound parameters have been suggested as biomarkers of the airway narrowing in children. Using a commercially available breath analyzer, characteristics condition were investigated infants with risk factors for asthma development.A total 443 (mean age, 9.9 months; range, 3-24 months) included present study. The frequency limiting 99% power spectrum (F99), roll-off from 600 to 1200 Hz (Slope) and curve indices, area under dBm data (A3/AT) ratio at 50% 75% highest (RPF75 RPF50),...
The common bed bug, Cimex lectularius harbors the endosymbiotic microorganism, Wolbachia (wCle), in a gonad-associated bacteriome as an obligate nutritional mutualist. obligatory nature of this association suggests that all individuals C. populations would be infected with wCle. However, studies spanning past several decades have reported variation both infection frequency and relative abundance wCle field-collected samples bugs. Since growth kinetics is poorly understood, objective study...
Abstract Background The common bed bug, Cimex lectularius , is an obligatory blood-feeding ectoparasite that requires a blood meal to molt and produce eggs. Their frequent biting obtain meals intimate association with humans increase the potential for disease transmission. However, despite more than 100 years of inquiry into bugs as vectors, they still have not been conclusively linked any pathogen or disease. This ecological niche extraordinarily rare, given nearly every other arthropod...
Viral infection is a major trigger for exacerbation of asthma and induces overproduction mucins. We investigated whether dsRNA could amplify the induction mucin by TGF-alpha in human bronchial epithelial cells, as well molecular mechanisms regulating MUC5AC expression. Human pulmonary mucoepidermoid carcinoma (NCI-H292) cells normal were exposed to polyinosinic-cytidyric acid (poly(I:C)) TGF-alpha. Then, protein production, mRNA expression, promoter activity evaluated. Cells pretreated with...