A5037118838- Vector-Borne Animal Diseases
- Animal Disease Management and Epidemiology
- Viral Infections and Vectors
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- CRISPR and Genetic Engineering
- Immune Cell Function and Interaction
- HIV Research and Treatment
- Cell Adhesion Molecules Research
- RNA and protein synthesis mechanisms
- vaccines and immunoinformatics approaches
- Malaria Research and Control
- Cytomegalovirus and herpesvirus research
- Microbial infections and disease research
- Complement system in diseases
- Virus-based gene therapy research
- Veterinary Oncology Research
- Animal health and immunology
- Immune Response and Inflammation
- Invertebrate Immune Response Mechanisms
- Infectious Diseases and Mycology
Ragon Institute of MGH, MIT and Harvard
2018-2024
Massachusetts General Hospital
2024
MRC University of Glasgow Centre for Virus Research
2015-2018
University of Milan
2013
Moredun Research Institute
2012
Engineering better bnAbs A highly effective HIV vaccine has been the goal of vaccinologists for nearly 35 years. successful would need to induce broadly neutralizing antibodies (bnAbs) that are capable multiple strains (see Perspective by Agazio and Torres). Steichen et al. report a strategy in which first shot can lead immune responses generate desired bnAbs. By combining knowledge human antibody repertoires structure guide design, they validated candidate immunogens through functional...
Viruses have often evolved overlapping reading frames in order to maximize their coding capacity. Until recently, the segmented dsRNA genome of viruses Orbivirus genus was thought be monocistronic, but identification bluetongue virus (BTV) NS4 protein changed this assumption. A small ORF segment 10, NS3 +1 position, is maintained more than 300 strains 27 different BTV serotypes and 200 phylogenetically related African horse sickness (AHSV). In BTV, (named S10-ORF2 study) encodes a putative...
Eliciting broadly neutralizing antibodies (bnAbs) is the core of HIV vaccine design. bnAbs specific to V2-apex region envelope acquire breadth and potency with modest somatic hypermutation, making them attractive vaccination targets. To evaluate Apex germline-targeting (ApexGT) candidates, we engineered knockin (KI) mouse models expressing germline B cell receptor (BCR) bnAb PCT64. We found that high affinity ApexGT immunogen for PCT64-germline BCRs was necessary specifically activate KI...
Abstract Current prophylactic human immunodeficiency virus 1 (HIV-1) vaccine research aims to elicit broadly neutralizing antibodies (bnAbs). Membrane-proximal external region (MPER)-targeting bnAbs, such as 10E8, provide exceptionally broad neutralization, but some are autoreactive. Here, we generated humanized B cell antigen receptor knock-in mouse models test whether a series of germline-targeting immunogens could drive MPER-specific precursors toward bnAbs. We found that recruitment 10E8...
Arboviruses cause acute diseases that increasingly affect global health. We used bluetongue virus (BTV) and its natural sheep host to reveal a previously uncharacterized mechanism by an arbovirus manipulate immunity. Our study shows BTV, similarly other antigens delivered through the skin, is transported rapidly via lymph peripheral nodes. Here, BTV infects disrupts follicular dendritic cells, hindering B-cell division in germinal centers, which results delayed production of high affinity...
Resource1 December 2020Open Access Transparent process Multiplexed CRISPR/CAS9-mediated engineering of pre-clinical mouse models bearing native human B cell receptors Xuesong Wang The Ragon Institute Massachusetts General Hospital, Technology and Harvard University, Cambridge, MA, USAThese authors contributed equally to this work Search for more papers by author Rashmi Ray Sven Kratochvil USA Eleonora Melzi orcid.org/0000-0002-1520-735X Ying-Cing Lin Sophie Giguere Liling Xu John Warner...
Repeat antigens, such as the Plasmodium falciparum circumsporozoite protein (PfCSP), use both sequence degeneracy and structural diversity to evade immune response. A few PfCSP-directed antibodies have been identified that are effective at preventing malaria infection, including CIS43, but how these repeat-targeting might be improved has unclear. Here, we engineered a humanized mouse model in which B cells expressed inferred human germline CIS43 (iGL-CIS43) cell receptors used vaccination...
Abstract Official guidelines do not consider bone marrow ( BM ) assessment mandatory in staging canine lymphoma unless blood cytopenias are present. The aim of this study was to find out if abnormalities can predict involvement large B‐cell lymphoma. infiltration assessed via flow cytometry. No difference found between dogs without haematological and with at least one. However, the degree significantly higher thrombocytopenia, leucocytosis or lymphocytosis negatively correlated platelet...
Resource7 August 2018Open Access Transparent process One-step CRISPR/Cas9 method for the rapid generation of human antibody heavy chain knock-in mice Ying-Cing Lin Ragon Institute MGH, MIT and Harvard, Cambridge, MA, USA Search more papers by this author Simone Pecetta Jon M Steichen Department Immunology Microbial Science IAVI Neutralizing Antibody Center, The Scripps Research Institute, La Jolla, CA, Sven Kratochvil Eleonora Melzi orcid.org/0000-0002-1520-735X Johan Arnold Stephanie K...
Arboviruses can cause a variety of clinical signs, including febrile illness, arthritis, encephalitis, and hemorrhagic fever. The recent Zika epidemic highlighted the possibility that arboviruses may also negatively affect male reproductive tract. In this study, we focused on bluetongue virus (BTV), causative agent one major ruminants. We show rams recovered from displayed signs testicular degeneration azoospermia up to 100 days after initial infection. Importantly, was induced in...
Sheep are not only a major livestock species globally, they also an important large animal model for biomedical research and have contributed to our understanding of the ontogeny architecture mammalian immune system. In this study, we applied immunohistochemistry multicolor immunofluorescence in fixed paraffin-embedded lymph nodes phenotype key populations antigen presenting cells, lymphocytes stromal cells that orchestrate host adaptive response. We used extensive panel antibodies directed...
Abstract Background Bovine neonatal pancytopenia (BNP) is a syndrome characterised by thrombocytopenia associated with marked bone marrow destruction in calves, widely reported since 2007 several European countries and 2011 New Zealand. The disease epidemiologically the use of an inactivated bovine virus diarrhoea (BVD) vaccine currently considered to be caused absorption colostral antibody produced some vaccinated cows (“BNP dams”). Alloantibodies capable binding leukocyte surface have been...