A5032286593- Metalloenzymes and iron-sulfur proteins
- Ion channel regulation and function
- Genomics and Chromatin Dynamics
- Metabolism and Genetic Disorders
- Microbial metabolism and enzyme function
- Asthma and respiratory diseases
- Enzyme Structure and Function
- Cell Image Analysis Techniques
- Amino Acid Enzymes and Metabolism
- CRISPR and Genetic Engineering
- Hemoglobin structure and function
- Ion Channels and Receptors
- 3D Printing in Biomedical Research
- RNA and protein synthesis mechanisms
- Ubiquitin and proteasome pathways
- Genetics, Aging, and Longevity in Model Organisms
- Inhalation and Respiratory Drug Delivery
- Ion Transport and Channel Regulation
- Bacterial Genetics and Biotechnology
- Chromosomal and Genetic Variations
- Cancer-related gene regulation
- Microbial Fuel Cells and Bioremediation
- Chemical Reactions and Isotopes
- Vanadium and Halogenation Chemistry
- Redox biology and oxidative stress
Aeon Imaging (United States)
2024
Amgen (Germany)
2014-2019
Amgen (United States)
2018
Brain (Germany)
2014
Ludwig-Maximilians-Universität München
2011
University of California, Berkeley
2002-2003
Howard Hughes Medical Institute
2002-2003
Max Planck Institute for Terrestrial Microbiology
1995-2000
Philipps University of Marburg
1995-1999
Max Planck Society
1998
There is an unmet need in severe asthma where approximately 40% of patients exhibit poor beta-agonist responsiveness, suffer daily symptoms and show frequent exacerbations. Antagonists the Ca2+-activated-Cl¯ channel, TMEM16A, offers a new mechanism to bronchodilate airways block multiple contractiles operating disease. To identify TMEM16A antagonists we screened library ~580,000 compounds. The anthelmintics niclosamide, nitazoxanide related compounds were identified as potent that blocked...
The 100 copies of tandemly arrayed Drosophila linker (H1) and core (H2A/B H3/H4) histone gene cluster are coordinately regulated during the cell cycle. However, molecular mechanisms that must allow differential transcription versus histones prevalent development remain elusive. Here, we used fluorescence imaging, biochemistry, genetics to show TBP (TATA-box-binding protein)-related factor 2 (TRF2) selectively regulates TATA-less Histone H1 promoter, while TBP/TFIID targets transcription....
Formylmethanofuran dehydrogenases are molybdenum or tungsten iron‐sulfur proteins containing a pterin dinucleotide cofactor. We report here on the primary structures of four subunits FwdABCD enzyme from Methanobacterium thermoautotrophicum which were determined by cloning and sequencing encoding genes fwdABCD. FwdB was found to contain sequence motifs characteristic for molybdopterin‐dinucleotide‐containing enzymes indicating that this subunit harbors active site. FwdA, FwdC FwdD showed no...
Methanobacterium thermoautotrophicum contains a tungsten formylmethanofuran dehydrogenase (FwdABCD) and molybdenum (FmdABC). The fwdHFGDACB operon encoding the enzyme has recently been characterized. We report here on structure expression of gene cluster enzyme. This is composed three open reading frames (fmdECB). fmdB was found to encode molybdopterin-dinucleotide-binding subunit harboring enzyme's active site; FmdB thus functionally equivalent FwdB. fmdC encodes protein with sequence...
Investigating molecular mechanisms underlying human taste sensation requires functionally dedicated and at the same time proliferating cells. Here, we isolated viable fungiform papillae cells from biopsy samples, adenovirally transduced proliferation promoting genes, obtained stably cell lines. Analysis of gene expression 1 line termed HTC-8 revealed that these express 13 TAS2R bitter receptor CD36, OXTR encoding oxytocin receptor, as well genes implicated with signal transduction fate...
Sweating is an important physiological process to regulate body temperature in humans, and various disorders are associated with dysregulated sweat formation. Primary secretion human eccrine glands involves Ca(2+) -activated Cl(-) channels (CaCC). Recently, members of the TMEM16 family were identified as CaCCs secretory epithelia; however, their molecular identity remained elusive. Here, we investigated function TMEM16A glands. Gene expression analysis revealed that expressed NCL-SG3 gland...
In Methanobacterium thermoautotrophicum , the fmdECB operon encoding molybdenum formylmethanofuran dehydrogenase is directly preceded by an open reading frame tfx predicted to encode a DNA binding protein. The 16.1 kDa protein has N‐terminal basic domain with helix–turn–helix motif for and C‐terminal acidic possibly transcriptional activation. We report here on properties of Tfx heterologously overproduced in Escherichia coli . was found bind specifically sequence downstream promoter operon,...
There is an unmet need in severe asthma where approximately 40% of patients exhibit poor β-agonist responsiveness, suffer daily symptoms and show frequent exacerbations. Antagonists the Ca 2+ -activated-Cl − channel, TMEM16A, offers a new mechanism to bronchodilate airways block multiple contractiles operating disease. To identify TMEM16A antagonists we screened library ~580,000 compounds. The anthelmintics niclosamide, nitazoxanide related compounds were identified as potent that blocked...