Background: Necroptosis, a cell death of caspase-independence, plays pivotal role in cancer biological regulation. Although necroptosis is closely associated with oncogenesis, metastasis, and immunity, there remains lack studies determining the necroptosis-related genes (NRGs) highly immunogenic type, kidney renal clear carcinoma (KIRC). Methods: The information clinicopathology transcriptome was extracted from TCGA database. Following division into train test cohorts, three-NRGs (TLR3,...

Enzyme-assisted ultrasonic extraction (EAUE) was utilized and optimized for extracting polysaccharides from Schizochytrium limacinum meal (SLMPs) via the response surface methodology. The optimal EAUE conditions were determined as follows: enzyme concentration at 5.18%, temperature 53 °C, duration of 40 min, power 60 W, a liquid-to-material ratio 34 mL/g, achieving polysaccharide yield 11.86 ± 0.61%. purified component, SLMP1-1, isolated using DEAE Sepharose Fast Flow Sephadex G-100 columns,...

Soy protein isolate (SPI) has promising applications in various food products because of its excellent functional properties and nutritional quality. The structural emulsifying covalently modified SPI by (-)-epigallocatechin-3-gallate (EGCG) were investigated.SPI was EGCG under alkaline conditions. SDS-PAGE analysis revealed that modification caused crosslinking proteins. Circular dichroism spectra demonstrated the secondary structure proteins changed modification. In addition, modifications...

Abstract As an energy‐saving and effective physical method, ultrasound technologies are gaining research attention for the production of modified starches. Corn starch–caffeic acid complexes prepared via process, effect power on physicochemical properties in vitro digestion is investigated. The results Fourier‐transform infrared spectroscopy amount analysis indicate that can help with formation complexes, caffeic depended power. Scanning electron microscopy shows exhibit obvious dense...

Thrombin is a critical serine protease in the coagulation system and widely used as target protein for antithrombotics. Spectroscopic analysis simple effective method that to study interaction between small molecules proteins. In this study, interactions of potential antithrombotic peptide AGFAGDDAPR (P10) with thrombin were investigated by fluorescence spectroscopy, UV-vis circular dichroism, Fourier-transform infrared spectroscopy Raman respectively. The results showed P10 bonded via...

Abstract The quality and shelf life of mushrooms are critical to their commercial viability. In this study, the effects simulated transport vibration on postharvest shiitake ( Lentinus edodes ) were assessed over 12 days storage. Furthermore, protective performance foam cushion material used in packaging during was evaluated. Changes respiration rate, weight loss, browning index, firmness, malondialdehyde content measured following treatment. results revealed that contributed deterioration...

This work was aimed at producing peptides containing iron-binding capabilities from scad (Decapterus maruadsi) processing by-product with alcalase hydrolysis. The chelating were purified by ultrafiltration, immobilized-metal affinity chromatography, gel filtration and reversed-phase high-performance liquid chromatography. A novel peptide 1,386.63 Da molecular weight amino acid sequence of QKGTYDDYVEGL. binds to iron mainly through carboxyl hydroxyl oxygen bonds. can significantly promote the...

Objective: To evaluate the efficacy and safety of a novel tip-flexible suction ureteral access sheath (NTFS-UAS) combined with flexible ureteroscopic lithotripsy (FURS) for treating kidney stones ≥30 mm in large cohort. Methods: The clinical data 206 patients renal calculi treated by NTFS-UAS FURS from June 2021 to September 2023 were analyzed retrospectively. outcomes under investigation encompassed demographic information, stone-related characteristics, operative time, stone-free rates...

The tumor suppressor gene pl6/MTSl, located on chromosome 9p21, is a cell cycle regulatory which frequently altered in human cancers. role of this prostate cancer unknown. To determine the frequency deletions and point mutations p16/MTS1 cancer, we examined 18 matched benign hyperplastic tissue specimens. Deletions pl6/MTS1 were detected by semi-quantitative multiplex polymerase chain reaction portion exon 2 control marker, glyceraldehyde 3-phosphate dehydrogenase gene, amplified...

Purpose: The interplay of inflammation and immunity affects all stages from tumorigenesis to progression, even tumor response therapy. A growing interest has been attracted the biological function MICALL2 its effects on progression. This study was designed verify whether could be a prognostic biomarker predict kidney renal clear cell carcinoma (KIRC) inflammation, immune infiltration within microenvironment (TME). Methods: We firstly analyzed expressions across 33 cancer types UCSC Xena...

In this paper, a fiber sensing system combined with loop ring down (FLRD) technique to detect aflatoxin B <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">1</sub> (AFB ) in food is proposed. The single-mode (SMF) taper was set as the sensor head, and waist diameter of it determined be 17.1 μm by refractive index (RI) measurement experiments. When light pulse enters circulates loop, experiences little loss each time passes through head. ring-down...

Oxalic acid and its oxalate salts have been linked to kidney stones other health problems about 80% are made up of calcium oxalate. Oxalyl coenzyme A decarboxylase (OXC) is a key enzyme in the catabolism In this study, we performed bioinformatic biochemical analysis OXC. First, mined OXC sequences from public protein database collected 1396 putative sequences. These were widely spread mainly distributed Actinobacteria, Alphaproteobacteria, Gammaproteobacteria, Betaproteobacteria classified...

Summary Inhibition of α‐glucosidases is recognised as an effective approach towards managing postprandial hyperglycaemia. This study aimed to identify a specific compound in Carya cathayensis Sarg. kernels (CCSKs) exhibiting strong inhibitory activities against α‐glucosidase and elucidate the kinetic mechanism. Employing bioassay‐guided approach, inhibitor was isolated from CCSKs through sequential chromatography protocol involving AB‐8 macroporous adsorptive resin, Sephadex LH‐20 C 18...

The aqueous enzymatic extraction (AEE) of oil from shrimp processing by-products was investigated. Four kinds proteases, including alkaline protease, neutral flavor protease and compound were applied to hydrolysis by-products. results showed that the best enzyme for obtain high recovery. influences four factors, amount, liquid/solid ratio, time temperature, on yield also studied. condition optimized as following: amount 2.0% (w/w), ratio 9.0ml/g, 2.6 h temperature 50 °C. Under these optimum...