A5014714289- Marine and coastal ecosystems
- Cannabis and Cannabinoid Research
- Aquatic Ecosystems and Phytoplankton Dynamics
- Pancreatic function and diabetes
- Sphingolipid Metabolism and Signaling
- Mycotoxins in Agriculture and Food
- Pesticide Residue Analysis and Safety
- Diet, Metabolism, and Disease
- Biocrusts and Microbial Ecology
- Plant Toxicity and Pharmacological Properties
- Inhalation and Respiratory Drug Delivery
- Plant and fungal interactions
- Cancer, Hypoxia, and Metabolism
- Autophagy in Disease and Therapy
- Aquatic Ecosystems and Biodiversity
- Alcohol Consumption and Health Effects
- Poisoning and overdose treatments
- Advanced Sensor Technologies Research
- Cholesterol and Lipid Metabolism
- Microbial Metabolism and Applications
- Sirtuins and Resveratrol in Medicine
- Chemical Reactions and Isotopes
- Cancer, Stress, Anesthesia, and Immune Response
- Scientific Measurement and Uncertainty Evaluation
- Phytochemicals and Medicinal Plants
Sciensano (Belgium)
2020-2025
Institut Scientifique de Santé Publique
2023
UCLouvain
2012-2021
Cliniques Universitaires Saint-Luc
2014
Obesity and its associated disorders are becoming a major health issue in many countries. The resulting low-grade inflammation not only affects the periphery but also central nervous system. We set out to study, time-dependent manner, effects of high-fat diet on different regions system with regard inflammatory tone. used diet-induced obesity model compared at several time-points (1, 2, 4, 6, 8, 16 weeks) group mice fed respective control standard diet. performed large-scale analysis lipids...
Significance 2-Arachidonoylglycerol (2-AG) is an endogenous bioactive lipid implicated in numerous (patho)physiological processes. 2-AG classically activates the cannabinoid receptors, and its activity terminated by enzymatic hydrolysis. The main enzyme studied this context monoacylglycerol lipase (MAGL). Although inhibition, to increase levels, has proven beneficial, it hindered psychotropic side effects due drastically elevated brain 2-AG. Here we show anti-inflammatory of inhibition...
The incidence of melanoma is considerably increasing worldwide. Frequent failing classical treatments led to development novel therapeutic strategies aiming at managing advanced forms this skin cancer. Additionally, the implication endocannabinoid system in malignancy actively investigated.We investigated cytotoxicity endocannabinoids and their hydrolysis inhibitors on murine B16 cell line using a MTT test. Enzyme receptor expression was measured by RT-PCR enzymatic degradation radiolabeled...
Monoacylglycerol lipase (MAGL) is responsible for signal termination of 2-arachidonoylglycerol (2-AG), an endocannabinoid neurotransmitter endowed with several physiological effects. Previously, we showed that the arylthioamide scaffold represents a privileged template designing MAGL inhibitors. A series 37 compounds resulting from pharmacomodulations around were synthesized and tested to evaluate their inhibitory potential on activity as well selectivity over fatty acid amide hydrolase...
Oxysterols are cholesterol derivatives that have been suggested to play a role in inflammatory diseases such as obesity, atherosclerosis, or neuroinflammatory diseases. However, the effect of neuroinflammation on oxysterol levels has only partially studied so far.We used an HPLC-MS method quantify over ten oxysterols both vitro and vivo models neuroinflammation. In same models, we RT-qPCR analyze expression enzymes responsible for metabolism. Using BV2 microglial cell line, explored...
In the context of increasing occurrences toxic cyanobacterial blooms worldwide, their monitoring in Belgium is currently performed by regional environmental agencies (in two three regions) using different protocols and restricted to some selected recreational ponds lakes. Therefore, a global assessment based on comparison existing datasets not possible. For this study, 79 water samples from five lakes Wallonia occasional Flanders Brussels, including canal, were analyzed. A Liquid...
Food supplements are gaining popularity worldwide. However, harmful natural compounds can contaminate these products. In the case of algae-based products, presence toxin-producing cyanobacteria may cause health risks. data about prevalence algal food on Belgian market and possible contaminations with cyanotoxins scarce. Therefore, we optimized validated a method based Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry to quantify eight microcystin congeners nodularin in...
Mycotoxins are naturally occurring secondary metabolites produced by specific fungal strains. They can cause adverse effects, posing a serious health threat to both humans and livestock. Focusing on several mycotoxins, this study first aimed at optimizing validating an ultra-high liquid chromatography-tandem mass spectrometry quantification method. This method was then applied evaluate the production of targeted mycotoxins in maize cultivated presence Aspergillus spp., Fusarium Alternaria...
Cyanotoxins can be produced in surface waters by cyanobacterial blooms, mostly during summer and early autumn. Intoxications would result from consumption of water contaminated with the potent hepatotoxins, microcystins nodularin. Therefore, WHO has set a guideline value for drinking quality concerning one congener microcystin. Consequently, design validated, public reference method to detect quantify hepatotoxins is necessary. During this study, was developed cyanotoxins (eight microcystin...
Apple and apple derivatives (e.g., juices, puree) are the most important foodstuffs contaminated with patulin (PAT) in human diet. To routinely monitor these ensure that PAT levels below maximum permitted levels, a method using liquid chromatography combined tandem mass spectrometry (LC-MS/MS) has been developed. Afterwards, was successfully validated, reaching quantification limits of 1.2 μg/L for juice cider, 2.1 μg/kg puree. Recovery experiments were performed samples fortified range...
Dairy milk holds a prominent position as widely consumed food, particularly among infants and children. However, it is crucial to address the presence of multiple natural toxic compounds that may co-occur in dairy ensure its safety prior consumption. Aflatoxin M1 (AFM1), an emerging mycotoxin interest, potential contaminant animals who ingest aflatoxin B1 (AFB1). The toxin regulated European Union under Commission Regulation No 1881/2006. Unfortunately, there notable lack data concerning...
Following pending new legislation in the European Union setting a maximum of 20 ng g-1 for total sum ergot alkaloids dry cereal-based baby food, UHPLC-MS/MS method was developed. It is suitable quantification six alkaloids: Ergocornine, ergocristine, ergometrine, ergosine, ergotamine, α-ergocryptine, and their corresponding epimers. The able to reliably detect individual at level as low 0.5 g-1. uses modified QuEChERS extraction approach before analysis. showed good sensitivity, accuracy,...
ABSTRACT Cancer-associated fibroblasts (CAFs) are the major cellular stromal component of many solid tumors. In prostate cancer (PCa), CAFs establish a metabolic symbiosis with PCa cells, contributing to aggressiveness through lactate shuttle. this study, we report that uptake alters NAD + /NADH ratio in which culminates SIRT1-dependent PGC-1α activation and subsequent enhancement mitochondrial mass activity. The high exploitation mitochondria results tricarboxylic acid cycle deregulation,...
The cholinic phenotype, characterized by elevated phosphocholine and a high production of total-choline (tCho)-containing metabolites, is metabolic hallmark cancer. It can be exploited for targeted therapy. Non-invasive imaging biomarkers are required to evaluate an individual's response anticancer agents that usually do not rapidly cause tumor shrinkage. Because changes manifest at earlier stages therapy than in size, the aim current study was (1)H-MRS diffusion-weighted MRI (DW-MRI) as...
Cardiac glycosides (CGs) are naturally occurring plant secondary metabolites that can be toxic to humans and animals. The aim of this work was develop a targeted analytical method utilizing liquid chromatography—tandem mass spectrometry (LC-MS/MS) for quantification these toxins in herbal-based food human urine. included oleandrin, digoxin, digitoxin, convallatoxin, ouabain. Samples culinary herbs were extracted with acetonitrile cleaned using Oasis® MAX solid-phase extraction (SPE), while...