A5039129582- Cancer, Hypoxia, and Metabolism
- Neonatal Respiratory Health Research
- Cardiovascular Function and Risk Factors
- Advanced MRI Techniques and Applications
- Tissue Engineering and Regenerative Medicine
- MRI in cancer diagnosis
- Cancer Cells and Metastasis
- Mitochondrial Function and Pathology
- Cancer Immunotherapy and Biomarkers
- Angiogenesis and VEGF in Cancer
- Caveolin-1 and cellular processes
- ATP Synthase and ATPases Research
- Cardiac Fibrosis and Remodeling
- Electrospun Nanofibers in Biomedical Applications
- Pancreatic function and diabetes
- Cystic Fibrosis Research Advances
- Mesenchymal stem cell research
- Asthma and respiratory diseases
- Medical Imaging Techniques and Applications
- Diet and metabolism studies
- Autophagy in Disease and Therapy
- Radiopharmaceutical Chemistry and Applications
- Metabolism, Diabetes, and Cancer
- Peroxisome Proliferator-Activated Receptors
- Pediatric health and respiratory diseases
UCLouvain
2016-2025
Directorate-General Joint Research Centre
2019-2025
Institut d'Imagerie Biomédicale
2024
Cliniques Universitaires Saint-Luc
2013-2022
IMEC
2019-2020
Imec the Netherlands
2020
KU Leuven
2004-2012
Ludwig-Maximilians-Universität München
2004-2011
University of Namur
2011
Vanderbilt University
2011
Metastatic progression of cancer is associated with poor outcome, and here we examine metabolic changes underlying this process. Although aerobic glycolysis known to promote metastasis, have now identified a different switch primarily affecting mitochondria. The involves overload the electron transport chain (ETC) preserved mitochondrial functions but increased superoxide production. It provides metastatic advantage phenocopied by partial ETC inhibition, another situation enhanced Both cases...
Gadolinium (Gd) Extracellular volume fraction (ECV) by Cardiovascular Magnetic Resonance (CMR) has been proposed as a non-invasive method for assessment of diffuse myocardial fibrosis. Yet only few studies used 3 T CMR to measure ECV, and the accuracy ECV measurements at not established. Therefore aims present study were validate measurement MOLLI T1 mapping against fibrosis measured histopathology. We also evaluated recently hypothesis that native-T1 without contrast injection would be...
Objective To investigate the beneficial role of prebiotics on endothelial dysfunction, an early key marker cardiovascular diseases, in original mouse model linking steatosis and dysfunction. Design We examined contribution gut microbiota to vascular dysfunction observed apolipoprotein E knockout (Apoe −/− ) mice fed n-3 polyunsaturated fatty acid (PUFA)-depleted diet for 12 weeks with or without inulin-type fructans (ITFs) supplementation last 15 days. Mesenteric carotid arteries were...
The activation of monocytes involves a stimulation glycolysis, release potent inflammatory mediators, and alterations in gene expression. All these processes are known to be further increased under hypoxic conditions. activated express inducible 6-phosphofructo-2-kinase (iPFK-2), which synthesizes fructose 2,6-bisphosphate, stimulator glycolysis. During ischemia, AMP-activated protein kinase (AMPK) activates the homologous heart isoform by phosphorylating its Ser-466. Here, we studied...
Background— In endothelial cells, caveolin-1, the structural protein of caveolae, acts as a scaffolding to cluster lipids and signaling molecules within caveolae and, in some instances, regulates activity proteins targeted caveolae. Specifically, different putative mediators endothelium-derived hyperpolarizing factor (EDHF)–mediated relaxation are located and/or regulated by such potassium channels, calcium regulatory proteins, connexin 43, molecular component gap junctions. Methods Results—...
Abstract Hypoxia is a common feature in tumors associated with an increased resistance of tumor cells to therapies. In addition O2 diffusion–limited hypoxia, another form hypoxia characterized by fluctuating changes pO2 within the disorganized vascular network described. Here, we postulated that this intermittent promotes endothelial cell survival, thereby extending concept hypoxia-driven vasculature. We found exposure cycles reoxygenation not only rendered them resistant proapoptotic...
Abstract Tumors may evade immune responses at multiple levels, including through a defect in the lymphocyte-vessel wall interactions. The angiogenic nature of endothelial cells (EC) lining tumor blood vessels account for such anergy. In this study, we examined whether mechanisms other than down-regulation adhesion molecules could be involved, particularly signaling pathways dependent on caveolae platforms. To mimic influence microenvironment, EC were exposed to TNF-α and proangiogenic...
In chronic obstructive pulmonary disease (COPD), epithelial changes and subepithelial fibrosis are salient features in conducting airways. Epithelial-to-mesenchymal transition (EMT) has been recently suggested COPD, but the mechanisms relationship to peribronchial remain unclear. We hypothesised that de-differentiation of COPD respiratory epithelium through EMT could participate airway thereby, obstruction. Surgical lung tissue primary broncho-epithelial cultures (in air-liquid interface...
Extracellular acidosis resulting from intense metabolic activities in tumors promotes cancer cell migration, invasion, and metastasis. Although host cells die at low extracellular pH, resist, as they are well equipped with transporters enzymes to regulate intracellular pH homeostasis. A further activates proteolytic that remodel the matrix facilitate migration invasion. Monocarboxylate transporter MCT1 is a passive of lactic acid has attracted interest target for small-molecule drugs prevent...
High lactate concentration in tumors is associated with bad prognosis. Lactate released by glycolytic cells and recaptured oxidative cancer to feed the tricarboxylic acid (TCA) cycle after conversion into pyruvate. Monocarboxylate transporters (MCT) mediate these fluxes of proton-linked represent attractive targets interrupt shuttle inhibit tumor growth. Here, we investigated properties 7-aminocarboxycoumarins (7ACC) developed selectively interfere lactate-rich microenvironment. The...
The generation of protective secretory IgA relies on the epithelial polymeric immunoglobulin receptor (pIgR). pIgR expression is reduced in chronic obstructive pulmonary disease (COPD), but correlation to severity and underlying mechanisms remains unknown.To address hypothesis that down-regulation COPD concerns severe relation aberrant programming bronchial epithelium.Surgical lung tissue primary epithelium (cultured air-liquid interface, ALI) obtained from a large series patients (n = 116)...
Abstract In COPD, epithelial changes are prominent features in the airways, such as goblet cell hyperplasia and squamous metaplasia. contrast, it remains unclear whether ciliated cells reduced which pathways dysregulate differentiation. We hypothesized that bronchial lineage specification is dysregulated COPD because of an aberrant reprogramming through transforming growth factor (TGF)-β1. Surgical lung tissue from 81 61 control (smokers non-smokers) patients was assessed for phenotyping by...
Abstract STUDY QUESTION How do elastic matrisome components change during the lifetime of human ovary? SUMMARY ANSWER The deposition and remodeling mechanical (collagen, elastin, elastin microfibril interface-located protein 1 (EMILIN-1), fibrillin-1 glycosaminoglycans (GAGs)) that play key roles in signaling pathways related to follicle activation development evolve an age- stage-related manner. WHAT IS KNOWN ALREADY mechanobiology ovary dynamic reciprocity exists between ovarian cells...
Pharmacological inhibition of AQP1 prevents H 2 O transport and hypertrophy cardiac myocytes.
Rationale: ACE2 (angiotensin-converting enzyme 2), the entry receptor for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is expressed in type alveolar epithelial cells (AT2) that may play key roles postinjury repair. An imbalance between and ACE has also been hypothesized to contribute lung injury. Objectives: To characterize expression distribution of compare AT2 with endothelial cell disease (COVID-19)-related or -unrelated distress (ARDS) controls. Methods: Lung tissue...
To decipher the mechanisms by which major human milk oligosaccharide (HMO), 2'-fucosyllactose (2'FL), can affect body weight and fat mass gain on high-fat diet (HFD) feeding in mice. We wanted to elucidate whether 2'FL metabolic effects are linked with changes intestinal mucus production secretion, mucin glycosylation degradation, as well modulation of gut microbiota, faecal proteome endocannabinoid (eCB) system.
BACKGROUND: Cardiac β3-adrenergic receptors (ARs) are upregulated in diseased hearts and mediate antithetic effects to those of β1AR β2AR. β3AR agonists were recently shown protect against myocardial remodeling preclinical studies improve systolic function patients with severe heart failure. However, the underlying mechanisms remain elusive. METHODS: To dissect functional, transcriptional, metabolic effects, isolated ventricular myocytes from mice harboring a moderate, cardiac-specific...
Encapsulating peritoneal sclerosis (EPS) is a rare but severe complication of dialysis (PD) characterized by extensive fibrosis the peritoneum. Changes in water transport may precede EPS, mechanisms and potential predictive value that defect are unknown. Among 234 patients with ESRD who initiated PD at our institution over 20-year period, 7 subsequently developed EPS. We evaluated changes time on these 28 matched controls using 3.86% glucose equilibration tests. Compared long-term controls,...
Antibody‐labeled gold nanoparticles represent a promising novel tool regarding cancer imaging and therapy. Nevertheless, the characterization of biodistribution such immunonanocarriers has been poorly documented. In this study, 89 Zr‐labeled cetuximab before after coupling reaction to (AuNPs) was compared quantitative performance Zr immuno‐PET evaluated. Cetuximab functionalized with desferal moiety labeled ( Zr–Df–Bz–NCS–cetuximab). AuNPs mean diameter 5 nm were synthesized according new...
The incidence of melanoma is considerably increasing worldwide. Frequent failing classical treatments led to development novel therapeutic strategies aiming at managing advanced forms this skin cancer. Additionally, the implication endocannabinoid system in malignancy actively investigated.We investigated cytotoxicity endocannabinoids and their hydrolysis inhibitors on murine B16 cell line using a MTT test. Enzyme receptor expression was measured by RT-PCR enzymatic degradation radiolabeled...
Introduction: Human ear reconstruction is recognized as the emblematic enterprise in tissue engineering. Up to now, it has failed reach human applications requiring appropriate complexity along with an accessible vascular tree. We hereby propose a new method process auricles order provide poorly immunogenic, complex and vascularized graft scaffold. Methods: 12 ears their pedicles were procured. Perfusion-decellularization was applied using SDS/polar solvent protocol. Cell antigen removal...
Chronic rhinosinusitis (CRS) defines a group of disorders characterized by persistent inflammation the sinonasal tract. Epithelial changes and structural remodelling are present, but whether epithelial differentiation is altered remains uncertain.To evaluate state epithelium in CRS, biopsies from patients with CRS nasal polyps (CRSwNP) or without (CRSsNP), allergic rhinitis (AR), as compared to controls, were processed immunohistochemistry RT-qPCR for terminal (E-cadherin, high molecular...
Human and mouse cardiac beta3-adrenergic receptors (beta3AR) exert antipathetic effects to those of beta1-2AR stimulation. We examined their role in modulating myocardial remodelling, particularly fibrosis response haemodynamic stress. Mice with myocyte-specific expression beta3AR (ADRB3-tg) or tamoxifen-inducible homozygous deletion (c-Adrb3-ko, loxP-targeted Adrb3) were submitted transaortic constriction. A superfusion assay was used for proteomic analysis paracrine mediators between...