A5014906789- RNA and protein synthesis mechanisms
- Computational Drug Discovery Methods
- Multiple Myeloma Research and Treatments
- SARS-CoV-2 and COVID-19 Research
- Immune Cell Function and Interaction
- interferon and immune responses
- Asthma and respiratory diseases
- Cancer Immunotherapy and Biomarkers
- Genetics, Bioinformatics, and Biomedical Research
- Machine Learning in Bioinformatics
- Peptidase Inhibition and Analysis
- Genomics and Phylogenetic Studies
- Ubiquitin and proteasome pathways
- Monoclonal and Polyclonal Antibodies Research
- Research on Leishmaniasis Studies
- Autoimmune Neurological Disorders and Treatments
- Herpesvirus Infections and Treatments
- RNA regulation and disease
- vaccines and immunoinformatics approaches
- Viral gastroenteritis research and epidemiology
- Advanced biosensing and bioanalysis techniques
- Mast cells and histamine
- Protist diversity and phylogeny
- Artificial Intelligence in Healthcare and Education
- Cancer Mechanisms and Therapy
Shenzhen Bay Laboratory
2021-2024
Shanghai Ocean University
2023
Zhejiang Lab
2023
Peking University
2020-2021
University of Maryland, College Park
2015-2019
Virginia–Maryland College of Veterinary Medicine
2015-2019
Soochow University
2014-2016
University of California, San Francisco
2013
Nanomed (Brazil)
2013
National Institute of Quality
2006
Abstract SARS-CoV-2 is the pathogen responsible for COVID-19 pandemic. The papain-like cysteine protease (PLpro) has been implicated in playing important roles virus maturation, dysregulation of host inflammation, and antiviral immune responses. multiple functions PLpro render it a promising drug target. Therefore, we screened library approved drugs also examined available inhibitors against PLpro. Inhibitor GRL0617 showed vitro IC 50 2.1 μM an effective inhibition cell-based assays....
<p>Artificial intelligence (AI) is driving transformative changes in the field of medicine, with its successful application relying on accurate data and rigorous quality standards. By integrating clinical information, pathology, medical imaging, physiological signals, omics data, AI significantly enhances precision research into disease mechanisms patient prognoses. technologies also demonstrate exceptional potential drug development, surgical automation, brain-computer interface (BCI)...
// Jinle Tang 1 , Jialu Li Xuejun Zhu 3 Yuan Yu Dan Chen 2 Lei 4 Zhenyang Gu Xingding Zhang 1, 2, 6 Lin Qi Zhishu Gong 7 Pengjun Jiang Juhua Huimin Meng Gangli An Huyong Zheng 5, * Yang 6, The Cyrus Hematology Center, Soochow University, Suzhou, Collaborative Innovation Center of Hematology, China Suzhou Cancer Immunotherapy and Diagnosis Engineering Division Department Medicine, Jiangsu Provincial Traditional Chinese Medical Hospital, Nanjing, Province, PLA General Beijing, 5 Beijing Key...
Bispecific antibodies play an important role in immunotherapy. They have received intense interest from pharmaceutical enterprises. The first antibody drug, OKT3 (muromonab-CD3), showed great performance clinical treatment. We successfully developed a single-chain variable fragment (ScFv) combination of anti-CD3 ScFv and anti-CD138 with the hIgG1 Fc (hIgFc) sequence. novel bispecific T-cell engager (BiTE) additional hIgFc (BiTE-hIgFc, STL001) can target T cells, natural killer multiple...
Human cytomegalovirus (HCMV) can persistently infect humans, but how HCMV avoids humoral immunity is not clear. The neonatal Fc receptor (FcRn) controls IgG transport from the mother to fetus and prolongs half-life. Here we show that US11 inhibits assembly of FcRn with β2m retains in endoplasmic reticulum (ER), consequently blocking trafficking endosome. Furthermore, recruits ubiquitin enzymes Derlin-1, TMEM129 UbE2J2 engage FcRn, initiating dislocation ER cytosol facilitating its...
Head and neck squamous cell carcinoma (HNSCC) is a prevalent cancer with poor response to conventional treatments such as surgery, chemotherapy radiotherapy. Immune checkpoint inhibitors (ICIs) have revolutionized the treatment of HNSCC, but many patients still exhibit responses due insufficient T infiltration impaired dendritic (DC) function within tumor microenvironment. DCs are crucial for initiating anti-tumor immune responses, their dysfunction in HNSCC leads inadequate activation...
Impairments in mitochondrial energy metabolism are thought to be involved many neurodegenerative diseases. The inhibitor 3-nitropropionic acid (3-NP) induces striatal pathology mimicking neurodegeneration vivo. Previous studies showed that 3-NP also triggered autophagy activation and apoptosis. In this study, we focused on the high-mobility group box 1 (HMGB1) protein, which is important oxidative stress signaling as well apoptosis, explore whether mechanisms of apoptosis diseases associated...
Significance The OAS–RNase L system is an innate immunity pathway activated by viral infection. Viral dsRNA stimulates OAS enzymes to produce short 2′,5′-oligoadenylates (2-5A) that activate RNase L, resulting in cleavage of single-stranded (ss) RNA. We discovered a small-molecule inhibitor rescues the toxic phenotype cells deficient dsRNA-editing enzyme ADAR1. ADAR1 destabilizes prevent activity. mutations are responsible for subset cases Aicardi-Goutières syndrome (AGS), severe...
Double-stranded DNA-specific cytidine deaminase (DddA) base editors hold great promise for applications in bio-medical research, medicine, and biotechnology. Strict sequence preference on spacing region presents a challenge DddA to reach their full potential. To overcome this sequence-context constraint, we analyzed protein dataset identified novel DddAtox homolog from Ruminococcus sp. AF17-6 (RsDddA). We engineered RsDddA mitochondrial editing mammalian cell line demonstrated RsDddA-derived...
The pseudokinase (PK) RNase L is a functional ribonuclease and plays important roles in human innate immunity. activity of can be regulated by the kinase inhibitor sunitinib. combined use oncolytic virus sunitinib has been shown to exert synergistic effects anticancer therapy. In this study, we aimed uncover mechanism action through which inhibits L. We solved crystal structures complex with its analogs toceranib SU11652. Our results showed that bound ATP-binding pocket Unexpectedly, αA...
ABSTRACT SARS-CoV-2 is the pathogen responsible for COVID-19 pandemic. The papain-like cysteine protease has been implicated in virus maturation, dysregulation of host inflammation and antiviral immune responses. We showed that PLpro preferably cleaves K48-ubiquitin linkage while also being capable cleaving ISG15 modification. multiple functions render it a promising drug target. Therefore, we screened an FDA-approved library examined available inhibitors against PLpro. Inhibitor GRL0617 IC...
The pseudokinase-endoribonuclease RNase L plays important roles in antiviral innate immunity and is also implicated many other cellular activities. inhibition of showed therapeutic potential for Aicardi-Goutières syndrome (AGS). Thus, a promising drug target. In this study, using an enzyme assay NMR screening, we discovered 13 inhibitory fragments against L. Cocrystal structures separately complexed with two different were determined which both bound to the ATP-binding pocket pseudokinase...
ABSTRACT High-resolution protein structure determination by experimental techniques is notoriously costly and labor intensive. This problem mostly solved with arrival of deep-learning-based computational prediction AlphaFold2 but only for those proteins enough naturally occurring homologous sequences. Here, we attempt to close the remaining gap employing artificially generated, structure-stability-selected sequences as an input AlphaFold2. We showed that one round selection deeply mutated a...
ABSTRACT Tertiary structures of RNAs are increasingly found to be essential for their functions and yet notoriously challenging determined by experimental techniques or predicted energy-based AlphaFold2-like deep-learning-based computational methods. Here, we coupled deep mutational scanning with mobility-based selection separate structurally stable from nonstable RNA variants. The subsequent high-throughput sequencing allows the detection covariational signals key secondary tertiary base...
Abstract The critical role of tumour antigen-specific immune responses to restrain growth in patients with gastric cancer furthers the need dissect co-inhibitory pathways involved infiltrating lymphocyte (TIL) exhaustion/dysfunction. It was previously reported that PD-1 expression significantly increased on CD4+ and CD8+ T cells from tissues, compared normal donors. In this study, we observed up-regulation TIM-3 peripheral CTLA-4, TIGIT, TILs patients; however TIGIT down-regulated not...
Abstract The human low affinity IgE receptor, CD23 (FceRII), is capable of transporting or IgE-allergen complexes across the polarized airway epithelial cell (AEC) monolayer in vitro. However, it remains completely unknown whether CD23-dependent transfer pathway AECs initiates and facilitates allergic inflammation vivo. To prove this, we first showed that wild-type (WT) mice transcytosed either ovalbumin (OVA)-IgE barrier. Neither type transcytosis was observed CD23-knockout (KO) mice. OVA...