A5101580848- Retinal Development and Disorders
- CRISPR and Genetic Engineering
- RNA regulation and disease
- Pluripotent Stem Cells Research
- Photochromic and Fluorescence Chemistry
- Neuroinflammation and Neurodegeneration Mechanisms
- Heme Oxygenase-1 and Carbon Monoxide
- Barrier Structure and Function Studies
- Neonatal Health and Biochemistry
- Cancer-related molecular mechanisms research
- Endoplasmic Reticulum Stress and Disease
- Multiple Sclerosis Research Studies
- Natural Antidiabetic Agents Studies
- NF-κB Signaling Pathways
- Ocular Oncology and Treatments
- Mechanisms of cancer metastasis
- Caveolin-1 and cellular processes
- RNA and protein synthesis mechanisms
- Photoreceptor and optogenetics research
- Alcohol Consumption and Health Effects
- Cancer-related Molecular Pathways
- Enzyme Structure and Function
- Muscle Physiology and Disorders
- Mitochondrial Function and Pathology
- Immune Response and Inflammation
Third Affiliated Hospital of Sun Yat-sen University
2007-2023
Sun Yat-sen University
2007-2023
Hefei University of Technology
2016
Health First
2016
Microglial activation plays an important role in neuroinflammation, which contributes to neuronal damage, and inhibition of microglial may have therapeutic benefits that could alleviate the progression neurodegeneration. Recent studies indicated antimalarial agent artemisinin has ability inhibit NF-κB activation. In this study, inhibitory effects on production proinflammatory mediators were investigated lipopolysaccharide (LPS)-stimulated primary microglia. Our results show significantly...
Urine cells, a body trash, have been successfully reprogrammed into human induced pluripotent stem cells (U-hiPSCs) which hold huge promise in regenerative medicine. However, it is unknown whether or to what extent U-hiPSCs can generate retinal so far. With modified differentiation protocol without addition of retinoic acid (RA), our study revealed that were able differentiate towards fates and form 3D organoids containing laminated neural retina with all cell types located proper layer as...
Abstract Retinoblastoma (RB) is the most common malignant tumor of retina in human children. Although it has been hypothesized for a long time that RB derives from multipotent retinal stem cells (RSCs) or retinoblasts, direct evidence presence tumorigenic RSCs tumors still lacking. Some studies indicate contain similar to their normal tissue cell counterparts. With vitro culture and differentiation method we demonstrate stem‐like (RSLCs) indeed exist lesions tumor‐derived cultures encompass...
RPE65-associated Leber congenital amaurosis (LCA) is one of highly heterogeneous, early onset, severe retinal dystrophy with at least 130 gene mutation sites identified. Their pathogenicity has not been directly clarified due to lack diseased cells. Here, we generated human induced pluripotent stem cells (hiPSCs) from putative LCA patient carrying two novel RPE65 mutations c.200T>G (p.L67R) and c.430T>C (p.Y144H), named RPE65-hiPSCs, which were confirmed contain the same mutations. The...
Induced pluripotent stem cells (iPSCs) have provided new opportunities for motor neuron disease (MND) modeling, drug screening, and cellular therapeutic development. Among the various types of iPSCs, urine-derived iPSCs become a promising source because they can be safely noninvasively isolated easily reprogrammed. Here, first time, we differentiated (urine-iPSCs) into neurons (MNs) compared capacity urine-iPSCs cord-blood-derived (B-iPSCs) to differentiate MNs. With use small molecules,...
Purpose: Retinal pigment epithelium (RPE) and neural retina could be generated concurrently through retinal organoid induction approaches using human induced pluripotent stem cells (hiPSCs), providing valuable sources for cell therapy of degenerations. This study aims to enrich expand hiPSC-RPE acquired with this platform explore characteristics serially passaged RPE cells. Methods: has been differentiated from hiPSCs a published method. After detachment on the 4th week, remaining mixture...
Scope The overproduction of very low density lipoprotein (VLDL) is an important cause for initiation and development atherosclerosis, which highly associated with insulin signaling. aim this work to verify whether the inhibition VLDL underlying mechanism a Laminaria japonica polysaccharide (LJP61A (where LJP L. )) resist atherosclerosis. Methods results LJP61A (50 200 mg/kg/day) was orally administered high‐fat diet (HFD)‐fed LDL receptor deficient mice 14 weeks. significantly attenuated...
Müller glial cells (MGCs) play important roles in human retina during physiological and pathological conditions. However, the development process of MGCs vivo remains unclear, how to obtain large numbers with high quality faces technical challenges, which hinder further study application MGCs. Human induced pluripotent stem cell (hiPSC)-derived retinal organoids (ROs) all subtypes provide an unlimited resource a platform for studies disorders. This explored hiPSC-derived ROs developed...
Background Bilirubin is the end product of heme catabolism and has strong antioxidant properties. Serum bilirubin levels are reported to be reduced in patients with multiple sclerosis (MS) neuromyelitis optica (NMO). The pathophysiology optic neuritis (ON) resembles that MS; however, role endogenous ON unclear. aim this study measure serum ON, investigate correlation between status bilirubin. Methods were measured 42 50 (MS), 48 (NMO) healthy control subjects. Results total (Tbil), direct...
To study the proteins related to paclitaxel-resistant of ovarian cancer cell line.The total and paclitaxel-sensitive human lines were separated by 2-dimensional gel electrophoresis (2-DE). The differentially expressed analyzed using image analysis software. differential identified matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Western blot was used determine expression levels 2 proteins.Forty found software, 24 These included proliferation nuclear antigen...
Glioblastoma (GBM) is the most lethal malignant primary brain tumor. Although multimodal therapy has been applied for GBM, median survival time remains less than 16 months. Thus, better therapeutic targets in GBM are urgently needed. Herein, we first identified five new N-terminal-truncated Cx32 isoforms (GJB1-28k, GJB1-22k, GJB1-20k, GJB1-15k, and GJB1-13k) further demonstrated that they were generated via cap-independent internal translation through ribosome entry sites (IRESs) coding...
CLCN2-related leukoencephalopathy (CC2L) is a rare disease due to autosomal recessive loss-of-function mutations in CLCN2 gene. We generated an induced pluripotent stem cell (iPSC) line (SKLOi001-A) from urine cells isolated CC2L patient carrying homozygotic mutation: c.2257C>T (p.Arg753*) gene via integration-free methods. The established iPSC kept the mutation and displayed normal karyotype, expressed pluripotency markers, showed differentiation potential. This newly could be served as...
Wolfram syndrome (WS), caused by mutations of the 1 (WFS1) gene on chromosome 4p16.1, is an autosomal recessive disorder characterized diabetes insipidus (DI), neuro-psychiatric disorders, hearing deficit, and urinary tract anomalies. Here we report a 11-year-old Chinese boy who presented with visual loss, was suspected optic neuritis (ON) or neuromyelitis optica (NMO) referred to our department for further diagnosis. Finally he diagnosed WS because mellitus (DM) atrophy (OA). Eight exons...
Abstract Background： The blood-brain barrier (BBB) and tight junction (TJ) proteins maintain the homeostasis of central nervous system (CNS). dysfunction BBB allows peripheral T cells infiltration into CNS contributes to pathophysiology multiple sclerosis (MS). Teriflunomide is an approved drug for treatment MS by suppressing lymphocytes proliferation. However, whether teriflunomide has a protective effect on in not understood. Methods： analysis patient samples, experiment autoimmune...