A5016723782- Chemical Synthesis and Analysis
- Supramolecular Self-Assembly in Materials
- Antimicrobial Peptides and Activities
- Advanced biosensing and bioanalysis techniques
- Lipid Membrane Structure and Behavior
- Molecular Sensors and Ion Detection
- Carbohydrate Chemistry and Synthesis
- Receptor Mechanisms and Signaling
- RNA modifications and cancer
- Bacteriophages and microbial interactions
- Helicobacter pylori-related gastroenterology studies
- Retinal Development and Disorders
- Infant Nutrition and Health
- Mass Spectrometry Techniques and Applications
- Analytical Chemistry and Chromatography
- Photosynthetic Processes and Mechanisms
- Fluorine in Organic Chemistry
- Protein Hydrolysis and Bioactive Peptides
- RNA and protein synthesis mechanisms
- Diatoms and Algae Research
- Microtubule and mitosis dynamics
- 14-3-3 protein interactions
- Protein Kinase Regulation and GTPase Signaling
- Ion channel regulation and function
- Cellular transport and secretion
Université Laval
2008-2023
PROTEO
2011-2023
Analogues of a synthetic ion channel made from helical peptide were used to study the mechanism cation translocation within bilayer membranes. Derivatives bearing two, three, four, and six crown ethers as relays synthesized, their transport abilities across lipid bilayers measured. The results showed that maximum distance sodium is permitted travel between two binding sites environment 11 Å.
We present experimental data that demonstrate the potential of synthetic crown ether modified peptide nanostructures to act as selective and efficient chemotherapeutic agents operate by attacking destroying cell membranes.
Toward developing nanostructures for single molecule detection, we report the synthesis of new derivatives artificial devices that possess ion channel activity. The membrane stability and transport ability these multiple crown α-helical peptides have been optimized by modulating polarity N- C-terminal groups with incorporation hydrophilic non-ionic units. Circular dichroism ATR studies confirmed conformation while 23Na NMR assays shown a significant increase their ability.
ABSTRACT Oriented circular dichroism was used to study the alignment crown ether‐modified peptides. The influence of different N‐ and C‐functionalities assessed using at variable peptide:lipid ratios from 1:20 1:200. Neither functionalities nor concentration had any major effect on orientation. 21‐mer peptides also examined with lipid membranes bilayer thickness. use synchrotron radiation as light source allowed molar 1:1000. For all conditions studied, were found be predominantly...
A novel 21-residue peptide incorporating six fluorinated amino acids was prepared. It designed to fold into an amphiphilic alpha helical structure of nanoscale length with one hydrophobic face and face. The formation a fluorous interface serves as the main vector for superstructure in bilayer membrane. Fluorescence assays showed this ion channel's ability facilitate translocation alkali metal ions through phospholipid membrane, selectivity sodium ions. Computational studies that tetramer is...
Abstract Fluorinated peptides attract much interest in the biomedical area because they generally exhibit an enhanced stability compared to their hydrogenated counterparts and fluorine atoms represent efficient probes investigate peptide assemblies, especially membranes. We previously designed characterized a fluorinated intended form ion channels This peptide, designated as LX2, adopts predominantly α‐helical structure acts selective channel for various cations. Molecular dynamics indicated...
Pierre-Alexandre Paquet-Côtéa, Matthieu Fillionb, Marie-Ève Provenchera, François Otisa, Justine Dionneb, Sébastien Cardinala, Barbara Collignonc, Jochen Bürckd, Patrick Lagüec, Anne S. Ulrichd, Michèle Augerb & Normand Voyer*aa Département de chimie and PROTEO, Université Laval, Québec, Canadab chimie, CERMA CQMF, Canadac biochimie, microbiologie et bio-informatique Canadad Institute of Biological Interfaces (IBG-2), Karlsruhe Technology (KIT), Karlsruhe, Germany
We report the design and synthesis of membrane-active peptide nanostructures, as well their use signal transducer in a fluorimetric assay for biologically relevant analytes. Addition hydrophobic 21-residue peptides bearing six crown ether side chains to solution small unilamellar vesicles loaded with pH-sensitive fluorophore induces rapid fluorescence increase associated Na<sup>+</sup>/H<sup>+</sup> transport across bilayer membrane. To demonstrate usefulness these nanostructures development...
Background: Retinol dehydrogenase 8 (RDH8) is a 312-amino acid (aa) protein involved in the visual cycle. Bound to outer segment disk membranes of photoreceptors, it reduces all-trans-retinal all-trans-retinol1 as one rate-limiting steps cycle2. RDH8 member short-chain dehydrogenase/reductase family. Its C-terminal allows its membrane-anchoring through postulated presence an amphipathic α-helix and 1 3 acyl groups at positions 299, 302 3043. The secondary structure membrane binding...