A5076070269- Hematopoietic Stem Cell Transplantation
- Mesenchymal stem cell research
- Cancer Genomics and Diagnostics
- Single-cell and spatial transcriptomics
- T-cell and B-cell Immunology
- Epigenetics and DNA Methylation
- Erythrocyte Function and Pathophysiology
- DNA Repair Mechanisms
- Acute Myeloid Leukemia Research
- Immune cells in cancer
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Science, Research, and Medicine
- Immunotherapy and Immune Responses
- Pluripotent Stem Cells Research
- Zebrafish Biomedical Research Applications
- Genetic factors in colorectal cancer
- Molecular Biology Techniques and Applications
- Blood disorders and treatments
- Kruppel-like factors research
- Cancer Cells and Metastasis
- Immune Cell Function and Interaction
- Eosinophilic Disorders and Syndromes
- Tryptophan and brain disorders
- Lipid metabolism and disorders
- Lymphatic System and Diseases
German Cancer Research Center
2015-2024
Heidelberg University
2016-2024
St Andrew's Healthcare
2023
Heidelberg Institute for Stem Cell Technology and Experimental Medicine
2018-2022
DKFZ-ZMBH Alliance
2015-2022
Open Geospatial Consortium
2021
International Society for Experimental Hematology
2019
University of Freiburg
2012-2013
University Medical Center Freiburg
2012-2013
Center for Clinical Research (United States)
2012
Abstract The rates and patterns of somatic mutation in normal tissues are largely unknown outside humans 1–7 . Comparative analyses can shed light on the diversity mutagenesis across species, long-standing hypotheses about evolution their role cancer ageing. Here we performed whole-genome sequencing 208 intestinal crypts from 56 individuals to study landscape 16 mammalian species. We found that was dominated by seemingly endogenous mutational processes all including 5-methylcytosine...
Iron is mostly devoted to the hemoglobinization of erythrocytes for oxygen transport. However, emerging evidence points a broader role metal in hematopoiesis, including formation immune system. availability mammalian cells controlled by iron-regulatory protein 1 (IRP1) and IRP2. We report that global disruption both IRP1 IRP2 adult mice impairs neutrophil development differentiation bone marrow, yielding immature neutrophils with abnormally high glycolytic autophagic activity, resulting...
The molecular etiology of myeloproliferative neoplasms (MPNs) remains incompletely understood, despite recent advances incurred through the discovery several different mutations in MPN patients. We have recently described overexpression transcription factor NF-E2 patients and shown that elevated levels vivo cause an phenotype predispose to leukemic transformation transgenic mice. report presence acquired insertion deletion gene These result truncated proteins enhance wild-type (WT) function...
The molecular pathophysiology of myeloproliferative neoplasms (MPNs) remains poorly understood. Based on the observation that transcription factor NF-E2 is often overexpressed in MPN patients, independent presence other aberrations, we generated mice expressing an transgene hematopoietic cells. These exhibit many features MPNs, including thrombocytosis, leukocytosis, Epo-independent colony formation, characteristic bone marrow histology, expansion stem and progenitor compartments,...
The proliferative activity of aging hematopoietic stem cells (HSCs) is controversially discussed. Inducible fluorescent histone 2B fusion protein (H2B-FP) transgenic mice are important tools for tracking the mitotic history murine HSCs in label dilution experiments. A recent study proposed that primitive symmetrically divide only four times to then enter permanent quiescence. We observed background fluorescence due leaky H2B-FP expression, occurring all transgenes independent induction,...
Within regenerating tissues, aging is characterized by a progressive general deterioration of organ function, thought to be driven the gradual depletion functional adult stem cells. Although there are probably multifactorial mechanisms that result in compromized cell functionality with advancing age, accumulation DNA damage within compartment likely make major contribution this process. However, physiologic source different tissue specific compartments remains determined, as does fate cells...
Abstract Hematopoietic stem cells (HSCs) are canonically defined by their capacity to maintain the HSC pool via self-renewal divisions. However, accumulating evidence suggests that function is instead preserved sustaining long-term quiescence. Here, we study kinetics of recovery in mice, following an inflammatory challenge induces HSCs exit dormancy. Repeated resulted a progressive depletion functional HSCs, with no sign later recovery. Underlying this observation, label retention...
Abstract The rates and patterns of somatic mutation in normal tissues are largely unknown outside humans. Comparative analyses can shed light on the diversity mutagenesis across species long-standing hypotheses regarding evolution their role cancer ageing. Here, we used whole-genome sequencing 208 intestinal crypts from 56 individuals to study landscape 16 mammalian species. We found be dominated by seemingly endogenous mutational processes all species, including 5-methylcytosine deamination...
Quantitative 3D imaging of organ-wide cellular and subcellular components is central for revealing understanding complex interactions between stem cells their microenvironment. Here, we present a gentle but fast whole-mount immunofluorescence staining protocol confocal microscopy (iFAST3D) that preserves the structure entire tissue structures with high fidelity. The iFAST3D enables reproducible high-resolution various niche many mouse organs tissues. For complete details on use execution...
Abstract The molecular etiology of polycythemia vera (PV) remains incompletely understood. Patients harbor increased numbers hematopoietic stem cells and display Epo-independent erythroid maturation. However, the mechanism underlying Epo hypersensitivity cell expansion is unclear. We have previously shown that transcription factor nuclear erythroid-2 (NF-E2) overexpressed in majority PV patients. Here we demonstrated elevation NF-E2 expression healthy CD34+ to levels observed caused...
Abstract The proliferative activity of adult hematopoietic stem cells (HSCs) is controversially discussed. Inducible fluorescent histone 2B fusion protein (H2B-FP) transgenic mice are important tools for tracking the mitotic history murine HSCs in label dilution experiments. A recent study proposed that most primitive divide only four times, to then enter permanent quiescence. We observed background fluorescence due leaky H2B-FP expression, occurring all transgenes independent induction,...
Professor Cecília Maria Arraiano directs a research group named 'Control of Gene Expression' at Instituto de Tecnologia Química e Biológica, Universidade NOVA Lisboa, Oeiras, Portugal. She started her scientific journey the University Lisbon, where she graduated in Biology, before completing PhD Genetics as Fulbright-Hays Fellow Georgia, Athens, USA. After postdoc USA, returned to Lisbon establish own lab. has authored close 200 publications mainly field RNA degradation mechanisms, with...
Hematopoietic stem cells (HSCs) are canonically defined by their capacity to maintain the HSC pool via self-renewal divisions. However, accumulating evidence suggests that function is instead preserved sustaining long-term quiescence. Here, we study kinetics of recovery in mice, following an inflammatory challenge induces HSCs exit dormancy. Repeated resulted a progressive depletion functional HSCs, with no sign later recovery. Underlying this observation, label retention experiments...
ABSTRACT Iron is mostly devoted to the hemoglobinization of erythrocytes for oxygen transport. Yet, emerging evidence points a broader role metal in hematopoiesis, including formation immune system. availability mammalian cells controlled by iron-regulatory proteins (IRP)-1 and −2. We report that global disruption both IRP1 IRP2 adult mice impairs neutrophil development differentiation bone marrow, yielding immature neutrophils with abnormally high glycolytic autophagic activity, resulting...