A5067748199- Herpesvirus Infections and Treatments
- Virus-based gene therapy research
- Nuclear Structure and Function
- RNA regulation and disease
- RNA Interference and Gene Delivery
- Viral Infections and Immunology Research
- HIV Research and Treatment
- Cognitive Abilities and Testing
- Extracellular vesicles in disease
- Language, Metaphor, and Cognition
- Nuclear Materials and Properties
- Nuclear and radioactivity studies
- Language, Communication, and Linguistic Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Education, Innovation and Language Studies
- Hepatitis Viruses Studies and Epidemiology
- Radar Systems and Signal Processing
- Emotional Intelligence and Performance
- Graphite, nuclear technology, radiation studies
- Cytomegalovirus and herpesvirus research
- MicroRNA in disease regulation
- Cellular transport and secretion
- interferon and immune responses
- Infrared Target Detection Methodologies
- RNA Research and Splicing
National Institute of Advanced Industrial Science and Technology
2022-2025
The University of Tokyo
2017-2022
National Institute of Infectious Diseases
2017-2022
Electronic Navigation Research Institute
1973
Vesicle-mediated nucleocytoplasmic transport is a nuclear pore-independent mechanism for the export of macromolecular complexes, but molecular basis this remains largely unknown. Here we show that endosomal sorting complex required transport-III (ESCRT-III) recruited to inner membrane (INM) during herpes simplex virus 1 (HSV-1). Scission HSV-1 budding through INM prevented by depletion ESCRT-III proteins. Interestingly, in uninfected human cells, proteins induces aberrant proliferation. Our...
ABSTRACT Although viral protein expression and progeny virus production were independently shown to be highly heterogeneous in individual cells, their direct relationship, analyzed by considering heterogeneities, has not been investigated date. To elucidate the relationship between production, we constructed a reporter herpes simplex 1 (HSV-1) tagging Venus late Us11. We then separated HSV-1-infected cell population into multiple subpopulations according fluorescence intensity of Venus—which...
Upon herpes simplex virus 1 (HSV-1) infection, the CD98 heavy chain (CD98hc) is redistributed around nuclear membrane (NM), where it promotes viral de-envelopment during egress of nucleocapsids. In this study, we attempted to identify factor(s) involved in CD98hc accumulation and demonstrated following: (i) null mutation HSV-1 UL34 caused specific dispersion throughout cytoplasm regulators, glycoproteins B H (gB gH); (ii) as observed with CD98hc, gB, gH, wild-type infection redistribution...
Hutchinson-Gilford progeria syndrome (HGPS) is a premature aging disorder, caused by mutation in the gene encoding lamin A/C, which produces truncated protein called progerin. In cells from HGPS patients, progerin accumulates at nuclear membrane (NM), where it causes NM deformations. this study, we investigated whether progerin-induced deformation involved ESCRT-III, complex that remodels and cytoplasmic membranes. The ESCRT-III CHMP4B was recruited to sites of aberrant proliferation human...
This study developed a system consisting of two rounds screening cellular proteins involved in the nuclear egress herpes simplex virus 1 (HSV-1). Using this system, we first screened that interacted with HSV-1 complex (NEC) UL34 and UL31 HSV-1-infected cells, which are critical for HSV-1, by tandem affinity purification coupled mass spectrometry-based proteomics technology. Next, performed CRISPR/Cas9-based live reporter cells under fluorescence microscopy using single guide RNAs targeting...
Herpes simplex virus (HSV) amplicon vector is a promising molecular tool with large transgene capacity of 150 kb. However, the conventional production method labor-intensive and expensive, limiting its application. Here, we generated HEK293 cells harboring HSV genome (293/HSV) developed an EASY-HSV (Efficient And Simple high-Yield Simplex Vector) system in which 293/HSV are transfected only two plasmids.
The authors have measured the echo power of ship target, Training Ship (G/T 360tons), as a function azimuth aspect angle at 4 miles range. result measurement and presumed Radar Cross Section are shown in Table 1, Fig.3 Fig.4. On results measurement, they point out follows ; (1) target is quite sensitive to aspect. minimum cross section occurs near head-on 360tons broadside roughly sixty times larger than that (2) received from almost never constant. chief source this fluctuation variations section.
ABSTRACT Although viral protein expression and progeny virus production were independently shown to be highly heterogenous in individual cells, their direct relationship, analyzed by considering heterogeneities, has not been investigated date. This study established a system fractionate cells infected with herpesvirus based on the levels of global late proteins, which are largely virion structural titrate yields these fractions. demonstrated relationship indicated there was threshold for...
Abstract In recent years, research has explored the use of microRNA (miRNA) analysis in extracellular vesicles (EVs) as a minimally invasive strategy for diagnosis and prediction diseases. This is because miRNAs EVs partly reflect miRNA information cellular status origin cells. However, not all intracellular are internalized into EVs. Therefore, obtained from limited. To get more information, we aimed to produce artificial (aEVs) encapsulating Argonaute 2 (Ago2) miRNA-binding protein, which...
原発性肺癌151例の血清CEA, フェリチン値とも非癌性胸部疾患に対し有意に高い陽性率を示した.特にCEA単独よりも, 両者の併用により肺癌での陽性率は高まった.CEA, フェリチンとも肺癌の進展の指標としても有用であった.胸水での検討では, CEAは癌性胸水で, ADAは結核性胸水でそれぞれ有意に高く, 両者の併用 (CEAは2.5ng/ml以上, ADAは50U/1以下) は癌性胸水での特異性を高め, 結核性胸水との鑑別に有用であった.