A5035965978- Spine and Intervertebral Disc Pathology
- Spinal Fractures and Fixation Techniques
- Hematopoietic Stem Cell Transplantation
- Polyomavirus and related diseases
- Musculoskeletal pain and rehabilitation
- Cytomegalovirus and herpesvirus research
- Acute Myeloid Leukemia Research
- Cervical and Thoracic Myelopathy
- Lymphoma Diagnosis and Treatment
- Vector-Borne Animal Diseases
- Herpesvirus Infections and Treatments
- T-cell and B-cell Immunology
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- T-cell and Retrovirus Studies
- Viral-associated cancers and disorders
- Transplantation: Methods and Outcomes
- Multiple Myeloma Research and Treatments
- Animal Disease Management and Epidemiology
- Blood disorders and treatments
- Urinary Bladder and Prostate Research
- Medical Imaging and Analysis
- Spinal Hematomas and Complications
- Scoliosis diagnosis and treatment
- Immune Cell Function and Interaction
- Pelvic floor disorders treatments
Kyoto University
2018-2025
Tokyo National Hospital
2025
Tohoku Medical and Pharmaceutical University
2025
National Agriculture and Food Research Organization
2023-2024
National Institute of Animal Health
2023-2024
The University of Tokyo
1996-2023
Kyoto University Hospital
2021-2023
Yamamoto Hospital
2023
Nippon Institute for Biological Science
2023
National Cancer Center
2022
Vesicle-mediated nucleocytoplasmic transport is a nuclear pore-independent mechanism for the export of macromolecular complexes, but molecular basis this remains largely unknown. Here we show that endosomal sorting complex required transport-III (ESCRT-III) recruited to inner membrane (INM) during herpes simplex virus 1 (HSV-1). Scission HSV-1 budding through INM prevented by depletion ESCRT-III proteins. Interestingly, in uninfected human cells, proteins induces aberrant proliferation. Our...
Lipid nanoparticles (LNPs) are one of the most successful technologies in messenger RNA (mRNA) delivery. While liver is frequent target for LNP delivery mRNA, delivering mRNA molecules to extrahepatic tissues also important. Herein, it reported on development an that targets secondary lymphoid tissues. New types alcohol-soluble phosphatidylserine (PS) derivatives designed as materials immune cells and then incorporated into LNPs using a microfluidic technique with high degree scalability...
Azacitidine is a mainstay of therapy for myelodysplastic syndrome (MDS)-related diseases. The purpose our study to elucidate the effect gene mutations on hematological response and overall survival (OS), particularly focusing their posttreatment clone size. We enrolled total 449 patients with MDS or related myeloid neoplasms. They were analyzed in pretreatment (n = 449) 289) bone marrow samples using targeted-capture sequencing assess impact size treatment outcomes. In Cox proportional...
African swine fever (ASF) is an infectious Suidae disease caused by the ASF virus (ASFV). Adaptation to less susceptible, non-target host cells one of most common techniques used attenuate virulent viruses. However, this may induce many mutations and large-scale rearrangements in viral genome, resulting immunostimulatory potential loss vivo. This study continuously maintained ASFV strain, Armenia2007 (Arm07), establish attenuated strain with minimum genetic alteration a susceptible cell...
African swine fever is a hemorrhagic viral disease with mortality rate of nearly 100% in pigs. Hence, it classified as notifiable by the World Organization for Animal Health. Because no field-available vaccine exists, virus (ASFV) control and eradication solely depend on good farm biosecurity management rapid accurate diagnosis. In this study, we developed new indirect serological enzyme-linked immunosorbent assay (ELISA) using recombinant p11.5 protein from ASFV solid-phase target antigen....
HSV-1 UL51 is conserved in all members of the Herpesviridae family. This viral protein phosphorylated and functions cell-cell spread cytoplasmic virion maturation HSV-1-infected cells. Although downstream effects have been clarified, there a lack information on how this regulated as well significance phosphorylation In study, we show that at Ser-184 promotes replication, spread, nuclear egress cell cultures pathogenicity mice. first report to identify mechanism by which infection. Our study...
Abstract Venetoclax plus azacitidine (VEN + AZA) is widely used in acute myeloid leukemia (AML). This study explored the role of static and dynamic profiles mutational clonal burden to predict outcomes by analyzing marrow samples from 228 VEN AZA treated AML cases at “Pre-treatment” ( n = 228), “Best-response” 105), “Relapse” 27) phases using targeted-capture sequencing. In a multivariate model, older age, prior AZA, TP53 mutation with variant allele frequency ≥0.10, RAS-pathway mutations...
Transforaminal lumbar interbody fusion (TLIF) is widely accepted for the treatment of arthrodesis. However, exact characteristics TLIF depend on number, location, shape, or materials implants, and type posterior instrument. Clinical biomechanical each procedure are still unclear. The present study investigated clinical radiological improvements after single level asymmetrical TLIF, in which a box-shaped spacer was obliquely inserted into intervertebral space, degenerative spondylolisthesis...
Abstract Identification of the complete set translated genes viruses is important to understand viral replication and pathogenesis as well for therapeutic approaches control infection. Here, we use chemical proteomics, integrating bio-orthogonal non-canonical amino acid tagging high-resolution mass spectrometry, characterize newly synthesized herpes simplex virus 1 (HSV-1) proteome in infected cells. In these cells, host cellular protein synthesis shut-off, increasing chance preferentially...
Transforaminal lumbar interbody fusion (TLIF) procedure is widely used, but the surgical indications for TLIF in elderly patients remain controversial because of potential risks such as inferior bone quality and higher rate postoperative complications. Clinical efficacy operative risk are unclear. This study investigated clinical effect safety degenerative spondylolisthesis with radiculopathy or neurogenic claudication aged 70 years older. The records were retrospectively reviewed 35...
Patients with recurrent adult T-cell leukemia/lymphoma (ATL) after allogeneic hematopoietic cell transplantation (allo-HCT) have a dismal prognosis. We retrospectively evaluated the safety and efficacy of lenalidomide (LEN) in 11 consecutive patients ATL allo-HCT. The median time from allo-HCT to recurrence was 111 days (range, 20-1476), that initiation LEN 162 43-1560). initial daily dose 10 mg 5-25), duration treatment 37 3-1078). Three (27%) achieved complete response two (18%) partial...
A 39-year-old man presented with an extremely rare discal cyst at the L3-4 level manifesting as a left L4 radiculopathy. Two months after onset, he suffered right radiculopathy new lumbar disc protrusion. Five medical treatment, patient's symptoms improved, and showed complete regression on magnetic resonance imaging. Most cases of are surgically treated, only two previous spontaneous regression. The present case suggests clinical radiological recovery symptomatic can be obtained by...
We describe 5 cases of osteochondroma (OC) originating from lumbosacral spine which caused radiculopathy. Four originated the lumbar spine; all L4 inferior articular process and presented L5 radiculopathy, other one case sacrum; S1 superior In cases, definitive diagnosis was made with histopathological findings; typical cartilaginous capping confirmed. The functional recovery completed in cases. As for imaging study, post myelography computed tomography revealed most diagnostic tool...
The herpes simplex virus 1 (HSV-1) Us8A gene overlaps the that encodes glycoprotein E (gE). Previous studies have investigated roles of in HSV-1 infection using null mutations and gE; therefore, role remains to be elucidated. In this study, we function its phosphorylation at serine 61 (Ser-61), which recently identified as a site by mass spectrometry-based phosphoproteomic analysis HSV-1-infected cells, pathogenesis. We observed (i) Ser-61 infected cells was dependent on activity...
VP26 is a herpes simplex virus 1 (HSV-1) small capsomere-interacting protein. In this study, we investigated the function of in HSV-1-infected cells with following results. (i) The null mutation significantly impaired incorporation minor capsid protein UL25 into nucleocapsids (type C capsids) nucleus. (ii) caused improper localization discrete punctate domains containing multiple proteins (e.g., VP5 major protein) nucleus; these corresponded to aggregates. (iii) packaging replicated viral...