A5046511815- Hematopoietic Stem Cell Transplantation
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Acute Myeloid Leukemia Research
- Erythrocyte Function and Pathophysiology
- Pancreatic function and diabetes
- Lymphoma Diagnosis and Treatment
- Metabolism, Diabetes, and Cancer
- Blood groups and transfusion
- Diabetes and associated disorders
- Growth Hormone and Insulin-like Growth Factors
- Hemoglobinopathies and Related Disorders
- Adipose Tissue and Metabolism
- Renal Transplantation Outcomes and Treatments
- Immunotherapy and Immune Responses
- Linguistics and Cultural Studies
- Gastrointestinal disorders and treatments
- Regulation of Appetite and Obesity
- CAR-T cell therapy research
- Chemical Synthesis and Analysis
- Hernia repair and management
- Congenital Diaphragmatic Hernia Studies
- Chronic Lymphocytic Leukemia Research
- Neuroendocrine Tumor Research Advances
- Streptococcal Infections and Treatments
Massachusetts General Hospital
2024-2025
Kyoto University
2008-2025
Kyoto University Hospital
2023
Shionogi (Japan)
1986-2021
RIKEN Center for Integrative Medical Sciences
2019
Kitano Hospital
2013-2016
Hiratsuka Kyosai Hospital
2013
Mie University Hospital
2007
Showa Pharmaceutical University
1995-2003
National Defense Medical College
1996-2000
Secondary lymphoid tissue chemokine (SLC) is a CC expressed mainly in lymph nodes, appendix and spleen, specifically chemotactic for lymphocytes (Nagira et al., J. Biol. Chem. 1997. 272: 19518-19524). Here, we carried out transendothelial migration assays to determine the classes subsets of migrating toward SLC. SLC attracted freshly isolated B cells with high efficiency T modestly. Thus, first strong activity on fresh cells. Among cell types subsets, broadly CD4+ CD8+ cells, CD45RO- (naive)...
The immune system encompasses acquired and innate immunity that matures through interaction with microenvironmental components. Cytokines serve as environmental factors foster functional maturation of cells. Although NOD/SCID/IL2rgKO (NSG) humanized mice support investigation human in vivo, a species barrier between cells the mouse microenvironment limits well function. To study roles cytokines cell development, we created NSG expressing hIL-7 hIL-15. alone was not sufficient for supporting...
A new type of mitogenic factor, termed MF, has been found in the culture supernatant Streptococcus pyogenes and its N‐terminal amino acid sequence determined. On basis this sequence, an S. gene encoding MF was cloned nucleotide The includes a long, open reading frame with 813 nucleotides capable precursor protein 271 acids. Removal putative 43 residues as signal peptide results mature 228 molecular mass is calculated 25,363 which consistent previously determined value 25,370 for secreted...
It has been demonstrated in vivo and vitro experiments that high-fat (HF) feeding causes insulin resistance. To elucidate the mechanism for this effect, we have measured kinase activity of receptor purified from livers HF-fed rats showed impaired action isolated rat adipocytes. In adipocyte experiments, HF led to a 65% decrease maximal response stimulated by 2-deoxyglucose uptake study. Although binding adipocytes also decreased 50% control due affinity, postbinding defect should be...
Human [LeuB-24]- and [LeuB-25]-insulins were semi-synthesized from porcine insulin by an enzyme-assisted coupling method. The receptor-binding ability of was 30--48% 2--5% respectively that human insulin. There no significant difference in degradation between these analogues on incubation with isolated adipocytes. decreased affinity due to increased dissociation rate rather than a change the association their binding cultured lymphocytes. negative co-operative effect [LeuB-25]-insulin 50 1%...
Background. The introduction of posttransplant cyclophosphamide (PTCy) for prophylaxis against graft-versus-host disease (GVHD) has led to an increase in the number transplants from haploidentical donors. Accordingly, we aimed understand impact HLA epitope mismatch on outcomes hematopoietic stem cell transplantation (HSCT) with GVHD. Methods. This retrospective study included 1037 patients who underwent their first HSCT hematologic malignancies peripheral blood donors a Japanese registry...
Abstract Molecular studies have highlighted recurrent alterations in epigenetic modifier genes, including TET2, DNMT3A, and IDH2, angioimmunoblastic T-cell lymphoma (AITL). However, the direct impact of gene regulatory elements on expression changes leading to lymphomagenesis remains poorly understood. We focus 3D chromatin organization. Hi-C RNA sequencing was performed diagnostic FFPE tumor samples from seventeen patients with AITL. Two CD4+ T cells individual donors one follicular helper...
There is no standard of care in relapsed/refractory T-cell/natural killer-cell lymphomas. Patients often cycle through cytotoxic chemotherapy (CC), epigenetic modifiers (EM) or small molecule inhibitors (SMI) empirically. Ideal therapy at each line remains unknown. We conducted a retrospective, multiple intervention, 'target-trial' using the PETAL global cohort. received front-line CC, then second and third (2L 3L) with either CC again, EM SMI (12 possible treatment scenarios). Overall...
Summary: The gene encoding a mitogenic factor, termed MF, was cloned from Streptococcus pyogenes and the recombinant MF overexpressed in Escherichia coli. Both natural had heat-resistant nuclease activity. activity of characterized using protein. showed endonuclease activity, digesting ssDNA, dsDNA tRNA. optimal pH for DNase 9.5. enhanced approximately tenfold by simultaneous presence two divalent cations, Mg 2+ Ca , compared to either alone inhibited EDTA or NaCI. heat stability biphasic;...
Graft-versus-host disease-free, relapse-free survival (GRFS) is a useful composite end point that measures without relapse or significant morbidity after allogeneic hematopoietic stem cell transplantation (allo-HSCT). We aimed to develop novel analytical method appropriately handles right-censored data and competing risks understand the risk for GRFS each component of GRFS. This study was retrospective data-mining on cohort 2207 adult patients who underwent their first allo-HSCT within Kyoto...
The CD34
A modified erythrocyte insulin receptor assay was developed. The major advantage of our method is the smaller amount blood necessary for and consequent simple handling in binding assay. Three ml enough this assay, making it possible to assess receptors a group pediatric patientts. There close relationship between mononuclear cells assays (r=0.900, p<0.05).With studies were done on normal subjects adult onset non-obese diabetics. Decreased seen diabetic group, due decreased number...
A new integron, located on the R plasmid of Pseudomonas aeruginosa, was isolated in Japan. This integron made up two conserved segments (5'-and 3'-conserved segments) and a single streptomycin resistance gene as cassette. The structure this resembles that InC, existence which postulated by Bissonnette Roy (J. Bacteriol. 174, 1248-1257, 1992).
The time-course of insulin binding to isolated adipocytes pretreated with chloroquine (0.1 mM) showed that no steady state condition was reached and the kept increasing time incubation up three hours. Sixty-one percent bound labeled could not be dissociated from these cells by 100 microgram/ml unlabeled insulin, whereas 86% control cells. Chromatography revealed intact which had ability bind receptors adipocytes, increased degraded decreased in treated However, normal stimulation...