A5041460017- Chromosomal and Genetic Variations
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Animal Genetics and Reproduction
- Pharmacogenetics and Drug Metabolism
- CRISPR and Genetic Engineering
- Computational Drug Discovery Methods
- Soybean genetics and cultivation
- Plant Molecular Biology Research
- Prenatal Screening and Diagnostics
- Reproductive biology and impacts on aquatic species
- Renal and related cancers
- RNA Interference and Gene Delivery
- Phytoestrogen effects and research
- Genomic variations and chromosomal abnormalities
- Single-cell and spatial transcriptomics
- Epigenetics and DNA Methylation
- Bioinformatics and Genomic Networks
- DNA Repair Mechanisms
- Genetic Neurodegenerative Diseases
- Cell death mechanisms and regulation
- Acute Kidney Injury Research
- Advanced Welding Techniques Analysis
- Sperm and Testicular Function
- Endoplasmic Reticulum Stress and Disease
- Welding Techniques and Residual Stresses
Southern Medical University
2025
Nanfang Hospital
2025
East China University of Science and Technology
2024
Northeast Agricultural University
2024
Ministry of Ecology and Environment
2024
Nanjing Institute of Environmental Sciences
2024
Chinese Academy of Sciences
2012-2023
Harvard University
2023
Boston Children's Hospital
2022-2023
Nanjing Agricultural University
2017-2023
Since the discovery of cell-free DNA (cfDNA) in maternal plasma, it has opened up new approaches for non-invasive prenatal testing. With development whole-genome sequencing, small subchromosomal deletions and duplications could be found by NIPT. This study is to review efficacy NIPT as a screening test aneuploidies CNVs 42,910 single pregnancies.A total pregnancies with different clinical features were recruited. The fetal was directly sequenced. Each chromosome...
Neuronal tau reduction confers resilience against β-amyloid and tau-related neurotoxicity in vitro vivo. Here, we introduce a novel translational approach to lower expression of the gene MAPT at transcriptional level using gene-silencing zinc finger protein transcription factors (ZFP-TFs). Following single administration adeno-associated virus (AAV), either locally into hippocampus or intravenously enable whole-brain transduction, selectively reduced messenger RNA by 50 80% out 11 months,...
Abstract Renal tubular epithelial cells (TECs) can initiate an adaptive response to completely recover from mild acute kidney injury (AKI), whereas severe often leads persistence of maladaptive repair and progression fibrosis. Through profiling active DNA regulatory elements by ATAC-seq, we reveal widespread, dynamic changes in the chromatin accessibility TECs after ischemia–reperfusion injury. We show that injury-specific domains become accessible prior gene activation, creating poised...
Huanglian ( Rhizoma Coptidis ) as a popular herb has been used for the treatment of various diseases such diarrhea, eye inflammation and women's abdominal ailments. Alkaloids are considered to be responsible its pharmacological effects. In this investigation, six alkaloids (coptisine, epiberberine, berberine, jateorrhizine, palmatine magnoflorine) were systematically evaluated their inhibition cytochrome P450 isoforms (CYP1A2, CYP2C8, CYP2C9, CYP2C19, CYP2D6 CYP3A4) in human liver microsomes...
The effects of regular organic solvents on the metabolic activities various human cytochromes P450 (P450s) have been reported. However, very little is known about their influence mediated by P450s in rat liver microsomes (RLM). purpose this study was to investigate such as methanol, acetonitrile, dimethyl sulfoxide (DMSO), acetone, and ethanol CYP1A, CYP2C, CYP2D, CYP2E, CYP3A-mediated metabolism using RLM. results showed that most enzymes appeared be solvent-dependent, tested probes were...
Diabetic kidney disease (DKD) is the leading cause of chronic (CKD) and end-stage renal (ESRD), but pathogenesis not completely understood. Tubulointerstitial injury plays critical roles in development progression DKD. The present study aimed to investigate profile tubulointerstitial immune cell infiltration reveal underlying mechanisms between tubular interstitial inflammation DKD using bioinformatics strategies. First, xCell analysis identified cells displaying significant changes...
Wild soybeans retain many substances significantly reduced or lost in cultivars during domestication. This study utilized LC-MS to analyze metabolites the seed coats and embryos of wild cultivated soybeans. 866 815 were identified extracts both soybean types, with 35 10 differing coat embryos, respectively. The upregulated are linked plant defense, stress responses, nitrogen cycling. MALDI-MSI results further elucidated distribution these differential cotyledons, hypocotyls, radicles. In...
Background: Cisplatin is a widely used chemotherapeutic drug, whereas the clinical application greatly limited by its nephrotoxic side effect. Currently, there has been no effective treatment to prevent cisplatin-induced acute kidney injury (cisplatin-AKI). Human amniotic epithelial cells (hAECs) and their derived exosomes (EXOs) have proven effectively protect against ischemia reperfusion-induced AKI, yet roles in cisplatin-AKI are still unknown. Methods: C57BL/6J mice were given two doses...
Abstract Background Optical genome mapping (OGM) is a novel assay for detecting structural variants (SVs) and has been retrospectively evaluated its performance. However, prospective evaluation in prenatal diagnosis remains unreported. This study aimed to prospectively assess the technical concordance of OGM with standard care (SOC) testing diagnosis. Methods A cohort 204 pregnant women was enrolled this study. Amniotic fluid samples from these were subjected SOC testing, which included...
Complex developmental mechanisms of vertebrates are unraveled using comparative genomic approaches. Several teleosts, such as zebrafish, medaka and pufferfish, used genetic model systems because they amenable to studies gene function. The rice field eel, a freshwater fish, is emerging specific system for vertebrate sexual development its small genome size naturally occurring sex reversal. Data presented here support the use eel another important fish studies, especially in development. This...
Summary Cellular lineage histories along with their molecular states encode fundamental principles of tissue development and homeostasis. Current lineage-recording mouse models have limited barcode diversity poor single-cell coverage, thus precluding use in tissues composed millions cells. Here, we developed DARLIN, an improved Cas9 barcoding line that utilizes terminal deoxynucleotidyl transferase (TdT) to enhance insertion events over 30 CRISPR target sites, stably integrated into 3...
Cultivated soybeans (Glycine max) and wild soja) are rich in terpenoids, however, the distribution of these terpenoids their seeds, particularly G. soja, remains unclear. In this study, metabolomics was used to analyze terpenoid spectra seed coats cotyledons max matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) employed image primary differential cotyledons. The analysis showed that had a higher content diterpenoids, which related flavor germination....