A5068069780- Respiratory Support and Mechanisms
- COVID-19 Clinical Research Studies
- Cardiac Arrest and Resuscitation
- Organ Transplantation Techniques and Outcomes
- Mechanical Circulatory Support Devices
- Long-Term Effects of COVID-19
- Renal Transplantation Outcomes and Treatments
- Liver Disease and Transplantation
- Antibiotic Resistance in Bacteria
- SARS-CoV-2 and COVID-19 Research
- Trauma and Emergency Care Studies
- Respiratory viral infections research
- Intensive Care Unit Cognitive Disorders
- Pulmonary Hypertension Research and Treatments
- Nitric Oxide and Endothelin Effects
- Liver Disease Diagnosis and Treatment
- Neuroscience of respiration and sleep
- Sepsis Diagnosis and Treatment
- Pneumocystis jirovecii pneumonia detection and treatment
- Emergency and Acute Care Studies
- Asthma and respiratory diseases
- Clinical Nutrition and Gastroenterology
- Thermal Regulation in Medicine
- Mycobacterium research and diagnosis
- Antibiotic Use and Resistance
University Hospital Leipzig
2014-2025
Leipzig University
2010-2024
University Hospital Heidelberg
2024
Universitätsmedizin Göttingen
2024
Integrated Oncology (United States)
2024
University of Cologne
2024
Heinrich Heine University Düsseldorf
2024
Düsseldorf University Hospital
2024
University Hospital Cologne
2024
Heidelberg University
2024
To investigate the immune response and mechanisms associated with severe coronavirus disease 2019 (COVID-19), we performed single-cell RNA sequencing on nasopharyngeal bronchial samples from 19 clinically well-characterized patients moderate or critical five healthy controls. We identified airway epithelial cell types states vulnerable to acute respiratory syndrome 2 (SARS-CoV-2) infection. In COVID-19, cells showed an average three-fold increase in expression of SARS-CoV-2 entry receptor...
Children have reduced severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection rates and a substantially lower risk for developing disease 2019 compared with adults. However, the molecular mechanisms underlying protection in younger age groups remain unknown. Here we characterize single-cell transcriptional landscape upper airways of SARS-CoV-2-negative (n = 18) age-matched SARS-CoV-2-positive 24) children corresponding samples from adults 44), covering an range 4 weeks to 77...
In coronavirus disease 2019 (COVID-19), hypertension and cardiovascular diseases are major risk factors for critical progression. However, the underlying causes effects of main anti-hypertensive therapies—angiotensin-converting enzyme inhibitors (ACEIs) angiotensin receptor blockers (ARBs)—remain unclear. Combining clinical data (n = 144) single-cell sequencing airway samples 48) with in vitro experiments, we observed a distinct inflammatory predisposition immune cells patients that...
Abstract Children are consistently reported to have reduced SARS-CoV-2 infection rates and a substantially lower risk for developing severe COVID-19. However, the molecular mechanisms underlying protection against COVID-19 in younger age groups remain widely unknown. Here, we systematically characterized single-cell transcriptional landscape upper airways negative age-matched positive children (n=42) corresponding samples from adults (n=44), covering an range of four weeks 77 years....
Mechanical ventilation is life-saving for patients with acute respiratory insufficiency. In a German prevalence study, 13.6% of in intensive care units received mechanical more than 12 hours; 20% these as treatment distress syndrome (ARDS). The new S3 guideline the first to contain recommendations entire process groups (indications, modes/parameters, ac- companying measures, treatments refractory impairment gas exchange, weaning, and follow-up care).
Recommendations concerning the management of hemoglobin levels and hematocrit in patients on extracorporeal membrane oxygenation (ECMO) still advise maintenance a normal hematocrit. In contrast, current transfusion guidelines for critically ill support restrictive practice. We report series receiving venovenous ECMO (vvECMO) acute respiratory distress syndrome (ARDS) treated according to regimen recommended patients. retrospectively analyzed 18 vvECMO due severe ARDS. Hemoglobin...
Abstract Background Management of critically ill nontrauma (CINT) patients in the resuscitation room emergency department (ED) is very challenging. Detailed data describing patient characteristics and management this population are lacking. This observational study describes epidemiology, outcome CINT ED room. Methods prospective, single center included all adult who were consecutively admitted to during 2 periods 1 year (September 2014–August 2015 vs. September 2017– August 2018). Patient...
This executive summary of a national living guideline aims to provide rapid evidence based recommendations on the role drug interventions in treatment hospitalized patients with COVID-19.
For patients with acute respiratory insufficiency, mechanical (“invasive”) ventilation is a fundamental therapeutic measure to ensure sufficient gas exchange. Despite decades of strong research efforts, central questions on therapy are still answered incompletely. Therefore, many different modes and settings have been used in daily clinical practice without scientifically sound bases. At the same time, implementation few evidence-based concepts (e.g., “lung protective ventilation”) into...
With most of the immunosuppressive protocols consisting calcineurin inhibitors (CI), nephrotoxicity has become a major long-term complication often compromising outcome. In single-center retrospective study, we reviewed 1173 liver transplantations to identify variables indicative for occurrence chronic renal dysfunction (CRD) (defined as > or = 1 episode serum creatinine increase 1.8 mg/dL 2 wk). Chronic was found in 137 (11.7%) all transplants [82 (7%) early (after 3-12 months), 55 (4.7%)...
Background Extracorporeal Membrane Oxygenation (ECMO) use is increasing despite limited evidence. The aim of this study was to demonstrate heterogeneity ECMO and its association with hospital size annual frequency in Germany. Methods This a database analysis all cases Germany from 2010 2016 using the German Diagnosis Related Groups (DRG) coding system for ECMO. Results During period, 510 hospitals performed 29,929 runs (12,572 vvECMO, 11,504 vaECMO, 1993 pECLA) an increase over time....
Abstract Molecular changes underlying the persistent health effects after SARS-CoV-2 infection remain poorly understood. To discern gene regulatory landscape in upper respiratory tract of COVID-19 patients, we performed enzymatic DNA methylome and single-cell RNA sequencing nasal cells patients ( n = 19, scRNA-seq 14) controls 14, 10). In addition, resampled a subset these for transcriptome analyses at 3 7) 12 months 5) post followed expression differentially regulated genes over time....
Objective . On 16 December 2006, most Eurotransplant countries changed waiting time oriented liver allocation policy to the urgency Model for End-stage Liver Disease (MELD) system. There are limited data on effects of this change within community. Patients and methods. A total 154 patients who had undergone deceased donor transplantation (LT) were retrospectively analyzed in three periods: period (1-year pre-MELD, n = 42) versus B post-MELD, 52) C (2 years after MELD implementation, 60)....
HMG-CoA-reductase inhibitors (statins) influence lipid metabolism and have pleiotropic effects. Several statins reduce various forms of pulmonary hypertension (PH) in animal models. The relationship between atorvastatin expression serotonin transporter protein (5-HTT) remains unknown. This study focused on the effects course monocrotaline (MCT)-induced PH its relation to 5-HTT expression. Male Sprague-Dawley rats were challenged with MCT or without subsequent daily oral treatment 0.1, 1, 10...
Abstract We studied the lung proteome changes in two widely used models of pulmonary arterial hypertension (PAH): monocrotaline (MCT) injection and chronic hypoxia (CH); untreated rats were as controls ( n = 6/group). After 28 days, invasive right ventricular systolic pressure (RVSP) was measured. Lungs immunostained for α‐smooth muscle actin (αSMA). 2‐DE 4/group) followed by nano‐LC‐MS/MS applied protein identification. Western blotting additionally if possible. RVSP significantly increased...
ABSTRACT The clinical course of COVID-19 is highly variable, however, underlying host factors and determinants severe disease are still unknown. Based on single-cell transcriptomes nasopharyngeal bronchial samples from clinically well-characterized patients presenting with moderate critical severities, we reveal the different types states airway epithelial cells that vulnerable for SARS-CoV-2 infection. In patients, observed a two- to threefold increase expressing entry receptor ACE2 within...
Information concerning the effects of genetic variation between different background strains on hemodynamic, morphometric, and gene expression response to hypoxia would be useful. Three mice were kept in phenotyped followed by profiling analysis. Among variables examined, hematocrit, right heart muscularization, ventricular systolic pressure showed a strain-specific effect. Increased inflammatory, muscle, angiogenesis genes seen all strains, though specific changed varied among groups. These...