A5069139292- Estrogen and related hormone effects
- Stress Responses and Cortisol
- Hypothalamic control of reproductive hormones
- Neuroscience and Neuropharmacology Research
- Menopause: Health Impacts and Treatments
- Receptor Mechanisms and Signaling
- Cholinesterase and Neurodegenerative Diseases
- Alzheimer's disease research and treatments
- Neuroendocrine regulation and behavior
- Hormonal Regulation and Hypertension
- Tryptophan and brain disorders
- Neuropeptides and Animal Physiology
- Advanced Fluorescence Microscopy Techniques
- Reproductive System and Pregnancy
- Biochemical effects in animals
- Nanoplatforms for cancer theranostics
- Luminescence Properties of Advanced Materials
- Perovskite Materials and Applications
- Retinal Development and Disorders
- Nerve injury and regeneration
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurogenesis and neuroplasticity mechanisms
- Ion channel regulation and function
- Circadian rhythm and melatonin
- Phytoestrogen effects and research
University of Pecs
2015-2023
University of Otago
2008-2020
Hungarian Academy of Sciences
1998-2010
Eötvös Loránd University
1984-2010
University of Pittsburgh
2008
University of Groningen
1998-2006
Babraham Institute
2002-2005
National Institute of Environmental Health Sciences
2003
HUN-REN Institute of Experimental Medicine
1996-2001
Czech Academy of Sciences, Institute of Experimental Medicine
1997
Abstract Whereas a cardinal role for β‐amyloid protein (Aβ) has been postulated as major trigger of neuronal injury in Alzheimer's disease, the pathogenic mechanism by which Aβ deranges nerve cells remains largely elusive. Here we report correlative vitro and vivo evidence that an excitotoxic cascade mediates neurotoxicity rat magnocellular nucleus basalis (MBN). In application to astrocytes elicits rapid depolarization astroglial membranes with concomitant inhibition glutamate uptake....
Estrogen exerts classical genomic as well rapid nongenomic actions on neurons. The mechanisms involved in estrogen signaling are poorly defined, and the roles of receptors (ERs alpha beta) unclear. We examined here vivo role ERs by evaluating estrogen-induced effects two major pathways within brains alphaER-, betaER-, double alphabetaER-knockout (ERKO) ovariectomized female mice. significantly (P < 0.05) increased numbers phospho-cAMP response element binding protein...
Abstract Fluorescence lifetime imaging provides more possibility of in vivo multiplexing second near infrared (NIR‐II) window. However, it still faces the obstacle that fluorescent probes with differentiable often exhibit quite different fluorescence intensity, especially short usually accompanies a weak resulting difficulty for simultaneously decoding multiplexed information due to interference background noise. To facilitate high‐fidelity imaging, we developed series Er 3+ doped double...
Abstract The amino acid γ-aminobutyric (GABA) plays an important role in the regulation of GnRH neurons. We examined whether GABA depolarizes or hyperpolarizes neurons over postnatal development using gramicidin, perforated-patch electrophysiology combined with GnRH-LacZ transgenic mice whom can be made to fluoresce. basic membrane properties and responsiveness were not altered by transgene expression fluorescence. Ten 12 immature (10–17 d) depolarized a direct dose-dependent manner that was...
The gonadal steroid estrogen exerts an important modulatory influence on the activity of multiple neuronal networks. In addition to classical genomic mechanisms action, also poorly understood rapid, nongenomic effects neurons. To examine whether may exert rapid actions intracellular signaling within gonadotropin-releasing hormone (GnRH) neurons in vivo ,we examined phosphorylation status cAMP response element-binding protein (CREB) these cells after administration 17-β-estradiol...
In addition to classical genomic mechanisms, estrogen also exerts nonclassical effects via a signal transduction system on neurons. To study whether has effect basal forebrain cholinergic system, we measured the intensity of cAMP response element-binding protein (CREB) phosphorylation (pCREB) in neurons after administration 17β-estradiol ovariectomized (OVX) mice. A significant time-dependent increase number pCREB-positive cells was detected medial septum-diagonal band (MS-DB) and substantia...
There is increasing recognition that estrogen exerts multifaceted regulatory effects on GnRH neurons. The acute of calcium dynamics in these cells were examined using a transgenic mouse line allows real-time measurement intracellular concentration ([Ca2+]i) neurons the brain slice preparation. 17-beta-Estradiol (E2) at 100 pm-100 nm was found to activate [Ca2+]i transients approximately 40% with an approximate 15-min latency. This effect not replicated by E2-BSA, which limits E2 action...
Sex differences exist in chronic pain pathologies, and gonadal estradiol (E2) alters the sensation. The nocisensor transient receptor potential vanilloid 1 (TRPV1) plays a critical role triggering pain. Here we examined impact of E2 on function TRPV1 mice sensory neurons vitro vivo. Both mechano- thermonociceptive thresholds plantar surface paw female were significantly lower proestrus compared with estrus phase. These higher ovariectomized (OVX) sham-operated This difference was absent...
The negative feedback mechanism through which 17β-estradiol (E2) acts to suppress the activity of GnRH neurons remains unclear. Using inducible and cell-specific genetic mouse models, we examined estrogen receptor (ER) isoforms expressed by that mediate acute feedback. Adult female mutant mice in ERα was deleted from all neonatal period failed exhibit estrous cycles or with neuronal ERβ deletion exhibited normal cycles, but a failure E2 LH secretion seen ovariectomized mice. Mutant...
Abstract In Alzheimer’s disease (AD), there is a loss in cholinergic innervation targets of basal forebrain which has been implicated substantial cognitive decline. Amyloid beta peptide (Aβ 1–42 ) accumulates AD that highly toxic for (BFC) neurons. Although the gonadal steroid estradiol neuroprotective, administration associated with risk off-target effects. Previous findings suggested non-classical action on intracellular signaling pathways ameliorative potential without estrogenic side...
The impact of glucocorticoids on β ‐amyloid (1–42) (A ) and NMDA‐induced neurodegeneration was investigated in vivo . A or NMDA injected into the cholinergic magnocellular nucleus basalis adrenalectomized (ADX) rats, ADX rats supplemented with 25%, 100%, 2×100% corticosterone pellets, sham‐ADX controls. ‐ damage neurones assessed by quantitative acetylcholinesterase histochemistry. Plasma concentrations fibre loss after injection showed a clear U‐shaped dose–response relationship. subsequent...
Abstract Postnatal handling, as a crucial early life experience, plays an essential role in the development of hypothalamo‐pituitary–adrenal axis responses to stress. The impact postnatal handling on reactivity stress‐related neuronal circuitries was investigated animals that were handled for first 21 days and adults they exposed physical (ether) or emotional (restraint) challenge. To assess activation we relied induction immediate‐early gene product c‐Fos analysed its spatial temporal...
Effect of ether stress on dialysate concentration extracellular amino acids in the hippocampus was studied by microdialysis freely moving rats that have been either sham operated (SHAM) or adrenalectomized and supplemented with subcutaneous steroid pellets (ADX+CORT) providing constant corticosterone (CORT) plasma levels. In SHAM rats, resulted a peak glutamate taurine 30 min after stress, while aspartate increased 120 challenge. These changes acid levels as well glutamate/glutamine ratio...
Somatostatin is an important mood and pain-regulating neuropeptide, which exerts analgesic, anti-inflammatory, antidepressant effects via its Gi protein-coupled receptor subtype 4 (SST4) without endocrine actions. SST4 suggested to be a unique novel drug target for chronic neuropathic pain, depression, as common comorbidity. However, neuronal expression cellular mechanism are poorly understood. Therefore, our goals were (i) elucidate the pattern of Sstr4/SSTR4 mRNA, (ii) characterize...