A5030561069- Lower Extremity Biomechanics and Pathologies
- Total Knee Arthroplasty Outcomes
- RNA Interference and Gene Delivery
- Synthesis and bioactivity of alkaloids
- Foot and Ankle Surgery
- Advanced Fluorescence Microscopy Techniques
- Photoreceptor and optogenetics research
- Advanced biosensing and bioanalysis techniques
- bioluminescence and chemiluminescence research
- Receptor Mechanisms and Signaling
- Knee injuries and reconstruction techniques
- Protein Kinase Regulation and GTPase Signaling
- Osteoarthritis Treatment and Mechanisms
- Galectins and Cancer Biology
- Hip disorders and treatments
- Glycosylation and Glycoproteins Research
- Synthesis and Biological Evaluation
- Sports injuries and prevention
- Click Chemistry and Applications
- Orthopaedic implants and arthroplasty
- Photochromic and Fluorescence Chemistry
- Protein purification and stability
- Cell Adhesion Molecules Research
- Retinal Development and Disorders
- Neuroscience and Neural Engineering
Xijing Hospital
2025
Anhui Medical University
2025
Third Hospital of Hebei Medical University
2019-2024
Hebei Medical University
2019-2024
Fujian Normal University
2024
Peking University
2004-2024
University of California, San Diego
2019-2024
Zhejiang Taizhou Hospital
2024
Wenzhou Medical University
2024
Integrated Chinese Medicine (China)
2024
Metabolic labeling of glycans with chemical reproters (e.g., alkyne or azide) in conjunction bioorthogonal chemistry is a powerful tool for imaging glycome; however, this method lacks protein-specificity and therefore not applicable to glycosylation specific protein interest (POI). Here we report the development cis-membrane FRET-based methodology that allows protein-specific on live cells. We exploit metabolic glycan site-specific simultaneously install FRET acceptor donor onto...
Abstract CD147 plays a critical role in the invasive and metastatic activity of malignant melanoma cells by stimulating surrounding fibroblasts to express matrix metalloproteinases vascular endothelial growth factor. We developed system that blocks human cell line, A375, using RNA interference. By transfecting with small interfering (siRNA) targets CD147, we were able establish two stable clones which expression was significantly down-regulated. This resulted decreased proliferation invasion...
Abstract Thousands of intracellular proteins are post‐translationally modified with O ‐GlcNAc, and O‐GlcNAcylation impacts the function mediates diverse biological processes. However, ubiquity this important glycosylation makes it highly challenging to probe state a specific protein at cellular level. Herein, we report development FLIM–FRET‐based strategy, which exploits spatial proximity ‐GlcNAc moiety attaching protein, for protein‐specific imaging in single cells. We demonstrated strategy...
Section:ChooseTop of pageAbstract <<MATERIALS AND METHODSRESULTSDISCUSSIONReferencesCITING ARTICLES
BACKGROUND: Long noncoding RNA MIAT expression is related to the development of some diseases. However, role in melanoma was has seldom been studied. OBJECTIVE: To investigate effect lncRNA on cells. METHOD: Microarray used analyze lncRNAs tissue samples. The detected by qRT-PCR. A CCK-8 assay assess cell viability, and counting proliferation. Wound healing Transwell invasion assays were detect migration abilities, respectively, Western blotting performed explore molecular mechanisms...
Membrane voltage is an important biophysical signal that underlies intercellular electrical communications. A fluorescent indicator presented enables the investigation of signaling at high spatial resolution. The method built upon site-specific modification microbial rhodopsin proteins with organic fluorophores, resulting in a hybrid scaffold one most sensitive and fastest orange-colored indicators developed to date. We applied this technique optically map connectivity cultured cells, which...
Aims The purpose of this study was to explore the correlation between femoral torsion and morphology distal condyle in patients with trochlear dysplasia lateral patellar instability. Methods A total 90 (64 female, 26 male; mean age 22.1 years (SD 7.2)) dislocation who were awaiting surgical treatment January 2015 June 2019 retrospectively analyzed. All underwent CT scans lower limb assess femur. at various levels assessed using a) anteversion angle (FAA), b) proximal angle, c)...
Abstract The regulation and function of the signaling adaptor protein p130 Cas in tumor cell anchorage‐independent survival, or anoikis resistance, were investigated human lung adenocarcinoma cells. tyrosine phosphorylation during detachment analyzed cells compared with that normal epithelial Cell trigged rapid dephosphorylation nontumorigenic anoikis‐sensitive cells, but had no effect on anoikis‐resistant Further analysis revealed total kinase activities associated are significantly higher...
The placenta forms the maternal-fetal interface to protect developing fetus from xenobiotics or pathogens. However, understanding of complex placental features and responses pathogens are hindered due lack near-physiological models. Here, we present an engineered human organoid microphysiological system (MPS) incorporated with vascular endothelium, which allows recapitulate early in a niche. MPS comprises customized insert-based organ chip rocker, enabling situ differentiation formation...
JNK is a member of the MAPK family and has essential roles in inflammation cell differentiation apoptosis. In recent years, there have been accumulating data indicating novel role for growth migration. this report, we demonstrate that activity necessary serotonin (5-HT)-induced proliferation migration bovine pulmonary artery smooth muscle cells (PASMCs). Stimulation with 5-HT was found to lead activation maximal at 10 min. Inhibition its specific inhibitor, SP-600125, or dominant-negative...