A5061343374- Microbial Metabolism and Applications
- Bacterial biofilms and quorum sensing
- Antimicrobial Resistance in Staphylococcus
- Enzyme-mediated dye degradation
- Clostridium difficile and Clostridium perfringens research
- Enzyme Production and Characterization
- Probiotics and Fermented Foods
- Heat shock proteins research
- Microbial Inactivation Methods
- Nematode management and characterization studies
- Listeria monocytogenes in Food Safety
- Plant-Microbe Interactions and Immunity
- Biofuel production and bioconversion
- Bacterial Genetics and Biotechnology
- Plant Pathogens and Fungal Diseases
- Viral gastroenteritis research and epidemiology
- Genomics and Phylogenetic Studies
- Dye analysis and toxicity
- Pharmaceutical and Antibiotic Environmental Impacts
- Microbial Metabolic Engineering and Bioproduction
- Biochemical and Structural Characterization
- Antibiotic Resistance in Bacteria
- Veterinary medicine and infectious diseases
- Bacillus and Francisella bacterial research
- Fungal Biology and Applications
Université Libre de Bruxelles
2012-2024
Centre d'Enseignement et de Recherches des Industries Alimentaires et Chimiques
2019-2022
Université du littoral côte d'opale
1998-2009
Brigham and Women's Hospital
2007
Inserm
2005
Institute for Biological Sciences
2005
Université de Caen Normandie
1996-2002
ABSTRACT Staphylococcus aureus and coagulase-negative staphylococci, primarily epidermidis , are recognized as a major cause of nosocomial infections associated with the use implanted medical devices. It has been established that clinical isolates often produce biofilm, which is involved in adherence to biomaterials provides enhanced resistance bacteria against host defenses antibiotic treatments. thought staphylococcal biofilm contains two polysaccharides, one responsible for primary cell...
Stress tolerance and cross-protection in Enterococcus faecalis ATCC19433 were examined after exposure to bile salts, acid or heat shock. Bile salts adapted cells demonstrated induced homologous cross-resistance. No of against stress is observed pretreatment with even sensitized the this challenge. Whole-cell protein extract analysis revealed that each treatment a battery proteins. Some these polypeptides are by more than one treatment. The greatest overlap between treatments. Eighteen...
The alkaline shock response in Enterococcus faecalis was studied this work. Cells adapted to an optimum pH of 10.5 were tolerate 11.9 conditions but acquired sensitivity acid damage. An analysis stress proteins revealed that 37 polypeptides amplified. Two these are DnaK and GroEL. combined results show bile salts responses closely related.
Enterococcus faecalisis a resident bacterium of the intestinal tract humans and animals. This can be responsible for serious diseases is one largest causes hospital-based infections. hardy organism resists many kinds stresses used as major indicator hygienic quality food, milk, drinking water. On other side, enterococci seem to have beneficial role in development cheese aroma are added certain starter cultures. Since ten years, our laboratory has two-dimensional electrophoresis (2-DE)...
The resistance to detergents and detergent-induced tolerance of a gastrointestinal organism, Enterococcus faecalis ATCC 19433, were examined. most remarkable observation was the rapid response cells in contact with bile salts sodium dodecyl sulfate (SDS). killing by high concentrations nearly instantaneous. A 5-s adaptation moderate sublethal or SDS (0.08 0.01%, respectively) sufficient induce significant against homologous lethal conditions (0.3% 0.017% SDS). However, subsequent challenge...
ABSTRACT The Enterococcus faecalis general stress protein Gsp65 has been purified from two-dimensional gel electrophoresis. Determination of its N-terminal sequence and characterization the corresponding gene revealed that gsp65 product is a 133-amino-acid sharing homologies with organic hydroperoxide resistance (Ohr) proteins. Transcriptional analysis gave evidence for monocistronic mRNA initiated 52 nucleotides upstream ATG start codon an induction in response to hydrogen peroxide, heat...
Summary: The response of Enterococcus faecalis ATCC 19433 to salt stress has been characterized previously in complex media. In this report, it demonstrated that bacterium actively accumulates the osmoprotectant glycine betaine (GB) from salt-enriched medium BHI. To further understand specific effects GB and other osmoprotective compounds adaptation salt-induced cross-tolerance lethal challenges, a chemically defined lacking putative osmoprotectants was used. medium, bacterial growth...
The DNA sequence of the genome Staphylococcus haemolyticus JCSC1435 revealed a putative capsule operon composed 13 genes in tandem. first seven (capABCDEFG(Sh)) showed > or = 57% similarity with aureus cap5 cap8 locus. However, capHIJKLM(Sh) are unique to S. and include encoding flippase, an aminotransferase, two glycosyltransferases, transcriptional regulator. Capsule-like material was readily apparent by immunoelectron microscopy on bacteria harvested postexponential phase growth. Electron...
Aims Evaluation of the antibacterial activity cultivable bacteria associated with marine sponges Hymeniacidon perlevis and Halichondria panicea against multi-drug–resistant Staphylococcus aureus. Methods Results One hundred fourteen bacterial isolates were recovered from H. panicea. Antibacterial action was demonstrated by 70% reference strain aureus ATCC 29213 31·6% Pseudomonas aeruginosa 27853 in agar overlay assays. potential further analysed 36 hospital strains diverse resistance...
ABSTRACTABSTRACTOptimization of xylanase and cellulase production by a newly isolated Aspergillus fumigatus strain grown on Stipa tenacissima (alfa grass) biomass without pretreatment was carried out using Box-Behnken design. First, the polysaccharides dried ground alfa grass were characterized chemical methods (strong diluted acid). The effect substrate particle size carboxymethylcellulase (CMCase) selected identified then investigated. Thereafter, experiments statistically planned with...
Relationship between intrinsic thermal resistance, thermotolerance and heat shock proteins (hsp) synthesis is studied in Enterococcus faecalis. We showed that an impressive phenotypic resistance was induced by mild a slight developed various sublethal pretreatments such as NaCl, SDS bile salts. Hydrogen peroxide, acid alkaline shifts or "thermomimetic" agent ethanol, did not enhance the survival of adapted cells against lethal (62 degrees C). The inhibition protein chloramphenicol rifampin...
Enterococci, fomerly confounded with faecal streptococci, are recognized since the beginning of century as being in origin and generally searched for waste waters food products; their detection may fact indicate presence enteropathogenic organisms. Although nearly ubiquitous, preferred ecological niche is intestine sphere. Rejected environment by means human faeces or animal dejecta, they scattered afterwards diverse niches. Once external environment, survival linked exceptional aptitude to...