A5061928795- Enzyme Production and Characterization
- Antibiotic Resistance in Bacteria
- Microbial Metabolites in Food Biotechnology
- Biofuel production and bioconversion
- Bacterial biofilms and quorum sensing
- Peptidase Inhibition and Analysis
- Diet, Metabolism, and Disease
- Antimicrobial Peptides and Activities
- Trace Elements in Health
- Vibrio bacteria research studies
- Cannabis and Cannabinoid Research
- Advanced Cellulose Research Studies
- Drug Transport and Resistance Mechanisms
- Chemical Synthesis and Analysis
- Pancreatic function and diabetes
- Enzyme Structure and Function
- Microbial Metabolism and Applications
- Pharmaceutical and Antibiotic Environmental Impacts
- Fungal Biology and Applications
- Pharmacology and Obesity Treatment
- Cassava research and cyanide
- Studies on Chitinases and Chitosanases
- Bacterial Genetics and Biotechnology
- Biochemical effects in animals
- Digestive system and related health
Centre d'Enseignement et de Recherches des Industries Alimentaires et Chimiques
2013-2014
Université Libre de Bruxelles
2008-2012
University of Liège
2003-2008
Institut de Chimie
2004
2-Arachidonoylglycerol plays a major role in endocannabinoid signaling, and is tightly regulated by the monoacylglycerol lipase (MAGL). Here we report crystal structure of human MAGL. The protein crystallizes as dimer, despite structural homologies to haloperoxidases esterases, it distinguishes itself wide hydrophobic access catalytic site. An apolar helix covering active site also gives insight into amphitropic character MAGL, likely explains how MAGL interacts with membranes recruit its...
Resistance nodulation cell division (RND)-based efflux complexes mediate multidrug and heavy-metal resistance in many Gram-negative bacteria. Efflux of toxic compounds is driven by membrane proton/substrate antiporters (RND protein) the plasma membrane, linked a fusion protein (MFP) to an outer-membrane protein. The three-component complex forms system that spans entire envelope. MFP required for assembly this proposed play important active role substrate efflux. To better understand MFPs...
Monoacylglycerol lipase (MAGL) is a key enzyme responsible for the termination of endocannabinoid signaling. Its crucial role in 2-arachidonoylglycerol (2-AG) metabolism, together with numerous pharmacological properties mediated by this endocannabinoid, emphasize interest MAGL as therapeutic target, along need to design potent and selective inhibitors. Meanwhile, complexity 2-AG degradation pathways underscores use purified source evaluation studies new We report here first heterologous...
The activity of class D β-lactamases is dependent on Lys70 carboxylation in the active site. Structural, kinetic and affinity studies show that this post-translational modification can be affected by presence a poor substrate such as moxalactam but also V117T substitution. Val117 strictly conserved hydrophobic residue located In addition, inhibition chloride ions due to competition between side chain carboxylate modified ions. Determination individual constants shows deacylation acyl-enzyme...
ABSTRACT The heterologous production in Escherichia coli , the purification, and kinetic characterization of four plasmid-encoded class C β-lactamases (ACT-1, MIR-1, CMY-2, CMY-1) were performed. Except for their instability, these enzymes are very similar to known chromosomally encoded AmpC β-lactamases. Their parameters did not show major differences from those obtained corresponding chromosomal enzymes. However, K m values CMY-2 cefuroxime, cefotaxime, oxacillin significantly decreased...
Several aminopeptidases of the M42 family have been described as tetrahedral-shaped dodecameric (TET) aminopeptidases. A current hypothesis suggests that these enzymes are involved, along with tricorn peptidase, in degrading peptides produced by proteasome. Yet remains ill defined, some members annotated cellulases because their homology CelM, formerly an endoglucanase Clostridium thermocellum. Here we describe catalytic functions and substrate profiles CelM TmPep1050, latter having...
RBcel1 is an endoglucanase belonging to glycoside hydrolase family 5 subfamily (GH5_5) that was recently identified from a soil metagenome library the Antarctic. Unlike its closest structural homologue (Cel5A Thermoascus aurantiacus), this enzyme reported be able catalyze transglycosylation reactions and has putatively been implicated in bacterial cellulose-synthesis process. Here, structure of at 1.4 Å resolution, solved by molecular replacement, reported. The putative substrate-binding...
ABSTRACT The ybxI gene of Bacillus subtilis 168 encodes a preprotein 267 amino acid residues, including putative signal peptide 23 residues. YbxI primary structure exhibits high similarity scores with two members the superfamily serine penicillin-recognizing enzymes: class D β-lactamases and hydrophilic carboxy-terminal domains BlaR MecR penicillin receptors. To determine function activity this enzyme, we have subcloned in pET-26b expression vector. Transformation Escherichia coli BL21(DE3)...
The discovery of new glycoside hydrolases that can be utilized in the chemoenzymatic synthesis carbohydrates has emerged as a promising approach for various biotechnological processes. In this study, recombinant Ps_Cel5A from Pseudomonas stutzeri A1501, novel member GH5_5 subfamily, was expressed, purified and crystallized. Preliminary experiments confirmed ability to catalyze transglycosylation with cellotriose substrate. crystal structure revealed several structural determinants around...
Surveys of beta-lactamases in different parts the world show an important increase class C beta-lactamases, thus study these enzymes is becoming issue. We created overproduction system for Mox-1, a plasmid beta-lactamase, by cloning gene encoding this enzyme, and placing it under control T7 promoter, using vector pET 28a. The purified ion exchange chromatography, was used to obtain molecular mass (38246), N-terminal sequence (GEASPVDPLRPVV), pI (8.9), perform detailed kinetic study....
The crystallographic structure of ACT-1, which is the first plasmid-mediated AmpC-type β-lactamase to have been completely analyzed in terms nucleotide sequence and has a high degree similarity chromosomal AmpC enzymes Enterobacter cloacae plasmid-encoded MIR-1, solved at 2.4 Å resolution. overall ACT-1 similar those other class C β-lactamases, such as from E. P99 Escherichia coli.