A5044373856- Lipid Membrane Structure and Behavior
- RNA and protein synthesis mechanisms
- Advanced NMR Techniques and Applications
- NMR spectroscopy and applications
- Electron Spin Resonance Studies
- ATP Synthase and ATPases Research
- Advanced biosensing and bioanalysis techniques
- Single-cell and spatial transcriptomics
- Crystallography and Radiation Phenomena
- Enzyme Structure and Function
- Advanced Chemical Physics Studies
- Various Chemistry Research Topics
- Sphingolipid Metabolism and Signaling
- Spectroscopy and Laser Applications
- Solid-state spectroscopy and crystallography
- DNA and Nucleic Acid Chemistry
- Metabolism and Genetic Disorders
- Lipid metabolism and biosynthesis
- Mass Spectrometry Techniques and Applications
- Influenza Virus Research Studies
- Renal and related cancers
- Cellular transport and secretion
- Protein Structure and Dynamics
Creighton University
2022-2025
École Normale Supérieure de Lyon
2019
Université Claude Bernard Lyon 1
2019
Centre National de la Recherche Scientifique
2019
University of Illinois Chicago
2014-2017
The highly conserved N-terminal 23 residues of the hemagglutinin glycoprotein, known as fusion peptide domain (HAfp23), is vital to membrane and infection mechanism influenza virus. HAfp23 has a helical hairpin structure consisting two tightly packed amphiphilic helices that rest on surface. We demonstrate new class amphipathic helix functions by leveraging negative curvature induced membranes. an inverted wedge shape characteristic lipids, with bulky hydrophobic region relatively small...
DNA packaged into cancer cell-derived EV is not well appreciated. Here, we uncovered signatures of secreted by pancreatic cells. The cells and non-cancer counterparts exhibit distinct low vs. high molecular weight (LMW HMW) fragments distribution, respectively. Genome sequencing Single Nucleotide Variants analysis revealed that 95% reads 94% SNVs map to noncoding regions the genome. Given ~1% human genome represents coding regions, 5% mapping rate suggests a non-random enrichment certain...
Abstract Narrow proton signals, high sensitivity, and efficient coherence transfers provided by fast magic‐angle spinning at magnetic fields make automated projection spectroscopy feasible for the solid‐state NMR analysis of proteins. We present first ultrahigh dimensional implementation this approach, where 5D peak lists are reconstructed from a number 2D projections protein samples different molecular sizes aggregation states, which show limited dispersion chemical shifts or inhomogeneous...
Residual Dipolar Couplings (RDCs) are integral to the refinement of membrane protein structures by NMR since they accurately define orientation helices and other structural units. Only a small set liquid crystals used for RDC measurements compatible with detergents needed in studies. The available detergent-compatible negatively charged, thus offering effectively only one five orthogonal components alignment Saupe matrix. In this communication, we present robust crystalline medium that is...
In vitro studies of protein structure, function, and dynamics typically preclude the complex range molecular interactions found in living tissues. vivo elucidate these relationships, yet they are incompatible with extensive controlled biophysical experiments available vitro. We present an alternative approach by extracting membranes from eukaryotic tissues to produce native bicelles capture rich environment while retaining advantages experiments. Native derived chicken egg or mouse cerebrum...
Isotropically tumbling discoidal bicelles are a useful biophysical tool for the study of lipids and proteins by NMR, dynamic light scattering, small-angle X-ray scattering. present low-curvature central region, typically enriched with DMPC in lamellar state, highly curved detergent rim, composed DHPC. In this report, we impact partitioning induced curvature few molecules foreign lipid on bicelle size, structure, curvature. Previous approaches studying have focused macroscopic bulk properties...
Hybrid NMR (hdNMR) is a powerful new tool that combines the strengths of solution- and solid-state to measure dipolar, chemical shift, quadrupolar tensors in aqueous solution. We introduce theory hdNMR partially randomly oriented (PRO) crystalline hydrogel samples. PRO samples produce spectra with characteristic Pake patterns from solid state, yet they maintain high-resolution dispersion solution experiments. With pulse sequences, we show how can be used high precision 1Hα-13Cα dipolar...
We have used velocity map ion imaging to measure the angular anisotropy of NO (A) products from photodissociation N2-NO complex. Our experiment ranged 108 758 cm-1 above threshold energy form + N2 (X) products, and these measurements reveal, for first time, a strong photodissociation. At threshold, we observed photoproducts recoil preferentially perpendicular laser polarization axis with an average parameter, β = -0.25; however, as available was increased, at energy, found +0.28. The changes...
The vivid colors of visible spectroscopy are amenable to classroom activities where students can view a demonstration and make observations. Here we report an active learning in-class activity that combines both absorption luminescence spectroscopy. is based on outreach involving glowing gelatin dessert. Students use absorbance spectra determine the optimal color prepare brightest most impactful demonstration. During activity, learn difference between emission relationship λmax observed...
Abstract Schmale Protonensignale, hohe Empfindlichkeit und effiziente Kohärenztransfers durch schnelles Drehen im magischen Winkel hohen Magnetfeldern ermöglichen eine automatisierte Projektionsspektroskopie in der Protein‐Festkörper‐NMR. Wir präsentieren die erste ultrahochdimensionale Implementierung dieses Ansatzes für Proteinproben mit unterschiedlicher Molekülgröße Aggregationsstufe. 5D‐Peaklisten werden aus einer Reihe von 2D‐Projektionen, begrenzte Dispersion chemischer Verschiebungen...