A5089727549- Neuroscience and Neuropharmacology Research
- Neurotransmitter Receptor Influence on Behavior
- Schizophrenia research and treatment
- Cannabis and Cannabinoid Research
- Mental Health Research Topics
- Receptor Mechanisms and Signaling
- Phytochemicals and Medicinal Plants
- Neuroinflammation and Neurodegeneration Mechanisms
- Forensic Toxicology and Drug Analysis
- Epilepsy research and treatment
- Neuroendocrine regulation and behavior
- Synthesis and Reactions of Organic Compounds
- Alcohol Consumption and Health Effects
- Neural dynamics and brain function
- Obsessive-Compulsive Spectrum Disorders
- Mental Health and Psychiatry
- Pain Mechanisms and Treatments
- Pharmacological Receptor Mechanisms and Effects
- Diet, Metabolism, and Disease
- Urinary Bladder and Prostate Research
- Pharmacological Effects and Toxicity Studies
- Treatment of Major Depression
- Growth Hormone and Insulin-like Growth Factors
- Neuropeptides and Animal Physiology
- Bipolar Disorder and Treatment
Istituto di Farmacologia Traslazionale
2012-2023
National Research Council
2015-2023
University of Cagliari
1995-2021
Ospedale Vincenzo Cervello
2017
National Academies of Sciences, Engineering, and Medicine
2009-2014
University of Bologna
2014
Sardegna Ricerche (Italy)
2007-2013
Weatherford College
2009
McGill University
2005
Institute of Genetic and Biomedical Research
2002
The compound N-piperidinyl-[8-chloro-1-(2,4-dichlorophenyl)-1,4,5,6-tetrahydrobenzo [6,7]cyclohepta[1,2-c]pyrazole-3-carboxamide] (NESS 0327) was synthesized and evaluated for binding affinity toward cannabinoid CB1 CB2 receptor. NESS 0327 exhibited a stronger selectivity receptor compared with N-piperidinyl-5-(4-chlorophenyl)-1-(2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide (SR 141716A), showing much higher (Ki = 350 +/- 5 fM 1.8 0.075 nM, respectively) the 21 0.5 nM 514 30...
Reduction in both presynaptic and postsynaptic structures the aging neocortex may significantly affect functional synaptic properties this area. To directly address issue, we combined whole-cell patch-clamp recording of spontaneously occurring currents (PSCs) with morphological analysis layer V pyramidal neurons parietal cortex young adult (1- to 2-month-old) aged (28- 37-month-old) BNxF344 F 1 hybrid rats. Analysis spontaneous PSCs was used contrast basal input structural alterations...
Previous reports have shown that the Delta(9)-tetrahydrocannabinol (Delta(9)TCH), major psychoactive cannabinoid components of marijuana, is able [corrected] to inhibit thyroid hormonal activity. The aim this study was characterize CB1 functional expression in rat by a multi-methods approach.RT-PCR used detect mRNA receptor (17.8+/-4.0% normalizing reference gene beta(2) microglobulin), as well endocannabinoid hydrolyzing enzyme, fatty acid amide hydrolase (46.9+/-4.3% gland. CB1-encoded...
The anti-inflammatory properties of the medicinal plant Withania somnifera (L.) Dunal (WS) are generally related to withanolides; consistently, several strategies under investigation increase concentration these compounds in WS extracts. However, a potential toxicity withanolides has been highlighted, thus questioning safety such preparations. At variance, relative contribution alkaloids is underrated, spite preliminary evidence underlining possible pharmacological relevance. Starting from...
Abstract In the present work, we investigated if an impairment of dopaminergic neurons after subchronic haloperidol treatment might be a possible physiopathologic substrate ‘early onset’ vacuous chewing movements (VCMs) in rats. For this purpose, different antipsychotics were used to analyse relationship between VCMs development and morphological alterations tyrosine‐hydroxylase‐immunostained (TH‐IM) neurons. Rats treated twice day with displayed significant increase that was both time‐ (2–4...
The effect on rat catalepsy induced by Δ 9 ‐tetrahydrocannabinol (Δ ‐THC) in association with haloperidol (HP) or clozapine (CLOZ) administration was investigated. ‐THC dose‐dependently increased HP (0.05–1 mg kg −1 , s.c.)‐induced catalepsy, while no observed after CLOZ (1–20 s.c.) ‐THC+CLOZ administration. CB 1 antagonist SR141716A (0.5–5 i.p.) reversed the increase mediated HP‐induced catalepsy. D 2 agonist quinpirole completely both and HP+Δ ‐THC; however, higher doses of were needed...
The effect of two α-adrenergic receptor antagonists widely employed in the therapy benign prostatic hyperplasia, tamsulosin [(-)-(<i>R</i>)-5-[2-[[2-(0-ethoxyphenoxy) ethyl]amino]propyl]-2-methoxybenzenesulfonamide] and alfuzosin [(±)-<i>N</i>-[3-[(4-amino-6,7-dimethoxy-2-quinazolinyl) methylamino]propyl] tetrahydro-2-furancarboxamide], was investigated rat vas deferens. Because several clinical studies have shown that causes ejaculatory disorders, this study also evaluated possible...
It is proposed that to achieve a therapeutic effect in schizophrenia patients, dopamine D(2)-receptor occupancy by antipsychotics within the striatum must exceed 60-65%. However, at high levels of occupancy, risk extrapyramidal symptoms (EPS) increased. Following oral dosing antipsychotics, peaks and troughs plasma drug concentrations may be mirrored fluctuations occupancy. Paliperidone, novel antipsychotic available as extended-release tablets (paliperidone ER), major active metabolite...
The expression of the 170-kDa (alpha) and 180-kDa (beta) isoforms DNA topoisomerase II (topo II) was investigated with two specific monoclonal antibodies (MoAbs) in human peripheral blood lymphocytes (PBL), before after phytohaemoagglutinin (PHA) stimulation. Binding each MoAb detected by indirect immunofluorescence labelling quantified flow cytometry. In resting PBL, intensity immunostaining very low for both isozymes; however, topo IIbeta-associated about 2.5 times significantly higher...
Withania somnifera (L.) Dunal extracts (WSEs) may possess therapeutic perspectives in the treatment of inflammation and pain. We aimed to evaluate antinociceptive property a WSE formalin test investigate involvement several neurotransmitter systems this effect. The time spent licking formalin-injected paw was recorded CD1 mice after pretreatment with increasing doses WSE. Also, c-Fos spinal cord expression effects different compounds were investigated under these experimental conditions....