A5022063584- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- MicroRNA in disease regulation
- Circular RNAs in diseases
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Cancer-related gene regulation
- Cancer, Hypoxia, and Metabolism
- RNA Research and Splicing
- Cancer-related Molecular Pathways
- Antimicrobial Peptides and Activities
- Cancer Cells and Metastasis
- Ubiquitin and proteasome pathways
- Hydrology and Watershed Management Studies
- Epigenetics and DNA Methylation
- Soil and Water Nutrient Dynamics
- Wnt/β-catenin signaling in development and cancer
- Extracellular vesicles in disease
- Mycobacterium research and diagnosis
- Environmental Quality and Pollution
- Cell Adhesion Molecules Research
- PI3K/AKT/mTOR signaling in cancer
- Supramolecular Self-Assembly in Materials
- Virus-based gene therapy research
- Fibroblast Growth Factor Research
China Three Gorges University
2014-2025
Jinan University
2017-2025
First Affiliated Hospital Zhejiang University
2025
Southern Medical University Shenzhen Hospital
2019-2025
Huazhong University of Science and Technology
2024
Guangzhou University of Chinese Medicine
2024
Second Affiliated Hospital of Zhejiang University
2014-2024
Huadong Hospital
2024
Fudan University
2015-2024
Helmholtz Zentrum München
2024
Esophageal cancer is one of the most lethal cancers worldwide with poor survival and limited therapeutic options. The discovery microRNAs created a new milestone in research. miR-377 located chromosome region 14q32, which frequently deleted esophageal squamous cell carcinoma (ESCC), but biological functions, clinical significance implication ESCC are largely unknown. In this study, we found that expression was significantly downregulated tumor tissue serum patients ESCC. Both levels were...
Objective: miR-126, the miRNA considered to be specially expressed in endothelial cells and hematopoietic progenitor cells, is strongly associated with angiogenesis.The purpose evaluate role of miR-126 hypoxia-induced angiogenesis possible mechanisms.Methods: The expression was detected hypoxia-treated RF/6A diabetic retinas using real-time PCR.The up-or down-regulated by transfecting miR-126-mimics or inhibitors into cells.Cell cycle analysis performed flow cytometry.The protein levels...
Abstract Our previous study demonstrated a progressive glycolytic perturbation during the course of DMBA-induced hamster oral carcinogenesis, which was attenuated by salvianolic acid B (Sal-B) treatment along with decreased incidences squamous cell carcinoma (OSCC) formation. It proposed that metabolic modulation should be an additional mode action attributable to Sal-B’s anti-carcinogenic activity. However, molecular mechanisms underlying Sal-B-induced function remained elusive. In present...
// Bin Li 1, 2, 3, 4 , Wen Xu Alfred King Y. Lam 5 Yang Wang 2 Hui-Fang Hu Xin Yuan Guan 6 Yan Ru Qin 7 Nassim Saremi Sai Wah Tsao 3 Qing-Yu He and Annie L. M. Cheung 1 School of Biomedical Sciences, Ka Shing Faculty Medicine, The University Hong Kong, Pokfulam, Kong SAR, China College Life Science Technology, Jinan University, Guangzhou, Centre for Cancer Research, Kong-Shenzhen Institute Research Innovation (HKU-SIRI), Shenzhen, Department Pathology, Griffith Medical Menzies Health...
Abstract Esophageal squamous cell carcinoma (ESCC) is one of the most common malignant tumors with poor survival. It urgent to search for new efficient drugs good stability and safety clinical therapy. This study aims identify potential anticancer from a compound library consisting 429 natural products. Echinatin, isolated Chinese herb Glycyrrhiza uralensis Fisch , was found markedly induce apoptosis inhibit proliferation colony-formation ability in ESCC. Confocal fluorescence microscopy...
Increasing attentions have been paid to the role of circRNAs in etiology triple-negative breast cancer (TNBC), and we strived figure out association circRNA AKT3/miRNA axis with TNBC chemo-resistance. Altogether 207 BC patients were divided into group (n=83) non-TNBC (n=124), MCF-10A, MDA-MB-231, MDA-MB-468, SK-BR-3 MCF-7 cell lines prepared advance. Expressions AKT3-derived relevant miRNAs tissues determined by employing real-time polymerase chain reaction (PCR). It was indicated that...
Cetuximab resistance is the main obstacle for treatment of EGFR overexpression cancer, including triple-negative breast cancer (TNBC). MicroRNA (miRNA)-155-5p upregulated in TNBC cells; thus, present study explored whether downregulation miR-155-5p enhanced anti-tumor effect cetuximab cells. MDA-MB-231 and MDA-MB-468 cells were infected with lentivirus-epidermal growth factor receptor (EGFR) 72 h to obtain EGFR-overexpressed cell lines (MDA-MB-231 MDA-MB-468). The inhibitory effects on...
// Bin Li 1,2,3 , Jin 1,7 Wen Xu 1,3 Xin Yuan Guan 2,4 Yan Ru Qin 5 Yi Zhang 4 Simon Law 2,6 Sai Wah Tsao 1,2 and Annie L.M. Cheung 1 Department of Anatomy, Ka Shing Faculty Medicine, The University Hong Kong, Pokfulam, Kong SAR, China 2 Centre for Cancer Research, 3 Kong-Shenzhen Institute Research Innovation (HKU-SIRI), Clinical Oncology, First Affiliated Hospital, Zhengzhou University, Zhengzhou, 6 Surgery, 7 Present Address: Center Molecular Medicine Austrian Academy Sciences, Vienna,...
// Wen Xu 1, 2 , Bin Li 2, 3 Alfred KY Lam 4 Sai Wah Tsao Simon YK Law 3, 5 Kwok Chan 6 Qiu Ju Yuan 1 Annie LM Cheung Department of Anatomy, Ka Shing Faculty Medicine, The University Hong Kong, Pokfulam, Kong SAR, China Kong-Shenzhen Institute Research and Innovation (HKU-SIRI), Centre for Cancer Research, Pathology, Griffith Medical School Health Institute, Gold Coast Campus, Coast, QLD 4222, Australia Surgery, Correspondence to: Cheung, e-mail: lmcheung@hku.hk Keywords: Tumor angiogenesis,...
Rationale: Dysregulated microRNA (miRNA) expressions in cancer can contribute to chemoresistance.This study aims identify miRNAs that are associated with fluorouracil (5-FU) chemoresistance esophageal squamous cell carcinoma (ESCC).The potential of miR-29c as a novel diagnostic, prognostic and treatment-predictive marker ESCC, its mechanisms therapeutic implication overcoming 5-FU were explored.Methods: The miRNA profiles an ESCC model acquired analyzed using Taqman microarray...
Abstract Metastasis accounts for 90% of cancer death worldwide, and effective therapeutic strategies are lacking. The aim this work is to identify the key drivers in tumor metastasis screen therapeutics treatment esophageal squamous cell carcinoma (ESCC). Gene Ontology analysis Cancer Genome Atlas (TCGA) gene expression datasets ESCC patients with or without lympy identifies that TGF β 2 highly enriched pathways essential upregulates metastatic tumors. High correlates patient survival,...
Cell invasion is a hallmark of metastatic cancer, leading to unfavorable clinical outcomes. In this study, we established two highly invasive lung cancer cell models (A549-i8 and H1299-i8) identified mesoderm-specific transcript (MEST) as novel regulator cancer. We aim characterize its biological function significance in metastasis.Transwell assay was performed establish high-invasive model. Immunohistochemistry (IHC) used detect MEST expression tumor tissues. Mass spectrometry bioinformatic...
The efficient delivery of nucleic acids as therapeutic agents is a major challenge in gene therapy. Peptides have recently emerged novel carrier for drugs and genes. C6M1 designed amphipathic peptide with the ability to form stable complexes short interfering RNA (siRNA). showed combination random coil helical structure water but mainly adopted conformation presence anions or siRNA. Revealed by dynamic light scattering (DLS) microscopy techniques, interaction siRNA HEPES led ∼70 ∼155 nm...
MiR‐3127‐5p has been implicated as a tumor‐suppressive microRNA (miRNA) in non‐small‐cell lung cancer (NSCLC) and its expression was associated with tumor recurrence poor prognosis. The aim of this study to determine whether miR‐3127‐5p regulates epithelial‐mesenchymal transition (EMT) NSCLC, investigate the underlying mechanisms. Using qRT‐PCR, we examined levels cohort primary NSCLC specimens without distant metastasis. We further performed series vitro vivo experiments effects mechanism...
5-Fluorouracil (5-FU) is a chemotherapeutic agent commonly used to treat esophageal squamous cell carcinoma (ESCC), but acquisition of chemoresistance frequently occurs and the underlying mechanisms are not fully understood. We found that microRNA (miR)-338-5p was underexpressed in ESCC cells with acquired 5-FU chemoresistance. Forced expression miR-338-5p these resulted downregulation Id-1, restoration both vitro vivo sensitivity treatment. The effects were abolished by reexpression Id-1....
Abstract Metastasis is the main factor of treatment failure in cancer patients, but underlying mechanism remains to be elucidated and effective new strategies are urgently needed. This study aims explore novel key metastasis-related microRNAs (miRNAs) esophageal squamous cell carcinoma (ESCC). By comparing miRNA profiles highly metastatic ESCC sublines, we established through serial vivo selection with parental cells, found that expression level miR-515-3p was lower tumor tissues than...
This investigation attempted to discern whether formononetin restrained progression of triple-negative breast cancer (TNBC) by blocking lncRNA AFAP1-AS1-miR-195/miR-545 axis. We prepared TNBC cell lines (i.e. MDA-MB-231 and BT-549) normal human mammary epithelial line MCF-10A) in advance, the were, respectively, transfected pcDNA3.1-lncRNA AFAP1-AS1, si-lncRNA pcDNA6.2/GW/EmGFP-miR-545 or pcDNA6.2/GW/EmGFP-miR-195. Resistance cells response 5-Fu, adriamycin, paclitaxel cisplatin was...
Since their development, cell-penetrating peptides (CPPs) have been used as delivery vehicles for various genetic or therapeutic agents; however, the uptake mechanisms of CPPs and details are still unclear. Understanding cellular internalization facilitate development gene vectors. In present study, we evaluated process a previously designed CPP, STR-KV, complexed with small interference RNA (siRNA) targeting at glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene. Using heparin treatment...