A5075257372- Tissue Engineering and Regenerative Medicine
- Pluripotent Stem Cells Research
- Heart Failure Treatment and Management
- Neuroscience and Neural Engineering
- Cardiovascular Function and Risk Factors
- Congenital heart defects research
- Cardiac electrophysiology and arrhythmias
- CRISPR and Genetic Engineering
- 3D Printing in Biomedical Research
- Cardiac pacing and defibrillation studies
- Electrospun Nanofibers in Biomedical Applications
- Cardiovascular Effects of Exercise
- Cardiac Structural Anomalies and Repair
- Cardiac Arrest and Resuscitation
- Cardiomyopathy and Myosin Studies
- COVID-19 epidemiological studies
- Cardiac Health and Mental Health
- Cardiac Valve Diseases and Treatments
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Atrial Fibrillation Management and Outcomes
- Chemotherapy-induced cardiotoxicity and mitigation
- Machine Learning in Healthcare
- Mechanical Circulatory Support Devices
- Hormonal Regulation and Hypertension
Technion – Israel Institute of Technology
2011-2025
Rambam Health Care Campus
2007-2025
Rappaport Family Institute for Research in the Medical Sciences
2007-2022
Université Paris-Sud
2020
University of California, San Diego
2019
Transplantation of a tissue-engineered heart muscle represents novel experimental therapeutic paradigm for myocardial diseases. However, this strategy has been hampered by the lack sources human cardiomyocytes and scarce vasculature in ischemic area limiting engraftment survival transplanted muscle. Beyond necessity endothelial capillaries delivery oxygen nutrients to grafted tissue, interactions between cardiomyocyte cells may also play key role promoting cell proliferation. In present...
The ability to derive human induced pluripotent stem (hiPS) cell lines by reprogramming of adult fibroblasts with a set transcription factors offers unique opportunities for basic and translational cardiovascular research. In the present study, we aimed characterize cardiomyocyte differentiation potential hiPS cells study molecular, structural, functional properties generated hiPS-derived cardiomyocytes.Cardiomyocyte was embryoid body system. Gene expression studies demonstrated that process...
An interatrial shunt may provide an autoregulatory mechanism to decrease left atrial pressure and improve heart failure (HF) symptoms prognosis.
Human embryonic stem cell-derived cardiomyocytes (hES-CMs) are thought to recapitulate the development of heart cells. Given exciting potential hES-CMs as replacement tissue in diseased hearts, we investigated pharmacological sensitivity and ionic current mid-stage (20-35 days post plating). A high-resolution microelectrode array was used assess conduction multicellular preparations spontaneously contracting embryoid bodies (EBs). TTX (10 microm) dramatically slowed velocity from 5.1 3.2 cm...
Human embryonic stem cells (hESC) are pluripotent lines that can differentiate in vitro into cell derivatives of all three germ layers, including cardiomyocytes. Successful application these unique the areas cardiovascular research and regenerative medicine has been hampered by difficulties identifying selecting specific cardiac progenitor from mixed population differentiating cells. We report generation stable transgenic hESC lines, using lentiviral vectors, single-cell clones express a...
Myocardial regeneration strategies have been hampered by the lack of sources for human cardiomyocytes (CMs) and significant donor cell loss following transplantation. We assessed ability a three-dimensional tissue-engineered vascularized cardiac muscle to engraft in vivo rat heart promote functional vascularization. Human embryonic stem cell-derived CMs alone or with endothelial cells (human umbilical vein cells) fibroblasts (triculture constructs) were seeded onto biodegradable porous...
Pro-arrhythmia (development of cardiac arrhythmias as a pharmacological side effect) has become the single most common cause withdrawal or restrictions previously marketed drugs. The development new medications, free from these effects, is hampered by lack an in vitro assay for human tissue. We hypothesized that embryonic stem cell-derived cardiomyocytes (hESC-CMs) assessed with combination cell electrophysiology and microelectrode array (MEA) mapping can serve novel model...
Arrhythmogenic right ventricular cardiomyopathy (ARVC) is a primary heart muscle disorder resulting from desmosomal protein mutations. ARVC characterized pathologically by fibrofatty infiltration and clinically arrhythmias sudden cardiac death. We aimed to establish patient-/disease-specific human induced pluripotent stem cell (hiPSC) model of ARVC.Dermal fibroblasts were obtained 2 patients with plakophilin-2 (PKP2) mutations, reprogrammed generate hiPSCs, coaxed differentiate into...
Background Acute kidney injury ( AKI ) following primary percutaneous coronary intervention pPCI is frequently interpreted as contrast‐induced but may result from other insults. We aimed to determine the causal association of contrast material exposure and incidence p PCI using a control group propensity score–matched patients with ST‐segment–elevation myocardial infarction who were not exposed material. Methods Results studied 2025 underwent 1025 receiving fibrinolysis or no reperfusion...
In heart failure (HF), implantable haemodynamic monitoring devices have been shown to optimize therapy, anticipating clinical decompensation and preventing hospitalization. Direct left-sided sensors offer theoretical benefits beyond pulmonary artery pressure systems. We evaluated the safety, usability, performance of a novel left atrial (LAP) system in HF patients.The VECTOR-HF study (NCT03775161) was first-in-human, prospective, multicentre, single-arm, trial enrolling 30 patients with HF....
Patients with heart failure (HF) are at an increased risk of hospital admissions. The aim this report is to describe the feasibility, safety and accuracy a novel wireless left atrial pressure (LAP) monitoring system in patients HF.The V-LAP Left Atrium Monitoring systEm for With Chronic sysTOlic & Diastolic Congestive Failure (VECTOR-HF) study prospective, multicenter, single-arm, open-label, first-in human clinical trial assess safety, performance usability (Vectorious Medical Technologies)...
Abstract Normothermic regional perfusion (NRP) allows assessment of therapeutic interventions prior to donation after circulatory death transplantation. Sodium-3-hydroxybutyrate (3-OHB) increases cardiac output in heart failure patients and diminishes ischemia–reperfusion injury, presumably by improving mitochondrial metabolism. We investigated effects 3-OHB on function transplanted hearts organoids. Donor pigs (n = 14) underwent followed NRP. Following static cold storage, were into...
Cell replacement strategies are promising interventions aiming to improve myocardial performance. Yet, the electrophysiological impact of these approaches has not been elucidated. We assessed consequences grafting two candidate cell types, that is, skeletal myoblasts and human embryonic stem cell-derived cardiomyocytes (hESC-CMs). The fluorescently labeled (DiO) cells were grafted into rat's left ventricular myocardium. Two weeks later, optical mapping was performed using...
Abstract Background COVID-19 outbreak poses an unprecedented challenge for societies, healthcare organizations and economies. In the present analysis we coupled climate data with spread rates worldwide, in a single country (USA). Methods Data of confirmed cases was derived from Global Cases by CSSE at Johns Hopkins University up to March 19, 2020. We assessed disease two measures: replication rate (RR), slope logarithmic curve cases, (RoS), linear regression curve. Results Based on...