Heart transplantation in donation after circulatory death (DCD) relies on warm perfusion using either situ normothermic regional (NRP) or ex machine perfusion. In this study, we explore an alternative: oxygenated hypothermic (HMP) a novel clinically applicable system, which is compared to NRP with static cold storage (SCS).In porcine model, DCD setting was simulated, followed by (1) and SCS (2) HMP the XVIVO preservation system (3) direct procurement (DPP) HMP. After preservation, heart...

Abstract Background Lactate is traditionally recognized as a by-product of anaerobic metabolism. However, lactate preferred oxidative substrate for stressed myocardium. Exogenous infusion increases cardiac output (CO). The exact mechanism underlying this has yet to be elucidated. aim study was investigate the cardiovascular mechanisms acute haemodynamic effects exogenous in an experimental model human-sized pigs. Methods In randomised, blinded crossover eight 60-kg-pigs, pigs received...

The ketone body 3-hydroxybutyrate (3-OHB) increases cardiac output (CO) by 35% to 40% in healthy people and with heart failure. mechanisms underlying the effects of 3-OHB on myocardial contractility loading conditions as well cardiovascular its enantiomeric forms, D-3-OHB L-3-OHB, remain undetermined.

Cardiogenic shock (CS) is associated with high mortality and medical therapies have failed to improve survival. Treatment lactate improved cardiac function which may benefit this condition. Comprehensive hemodynamic assessment of administration in CS lacking, the mechanisms underlying cardiovascular effects not yet been elucidated. In study we aimed cardiometabolic treatment experimental ischemic CS. a randomized, blinded design, 20 female pigs (60 kg) were studied. Left main coronary artery...

The cerebral effect of clamping following normothermic regional perfusion (NRP) in donation after circulatory death (DCD) remains unknown. We investigated the reperfusion during NRP and preventive on brain function a porcine model.In 16 pigs, intracranial physiological parameters were recorded, including pressure, blood (CBF), temperature, oxygen. Additionally, electroencephalography (EEG) somatosensory evoked potentials (SSEPs) used to assess function. animals cannulated for heart-lung...

Abstract Normothermic regional perfusion (NRP) allows assessment of therapeutic interventions prior to donation after circulatory death transplantation. Sodium-3-hydroxybutyrate (3-OHB) increases cardiac output in heart failure patients and diminishes ischemia–reperfusion injury, presumably by improving mitochondrial metabolism. We investigated effects 3-OHB on function transplanted hearts organoids. Donor pigs (n = 14) underwent followed NRP. Following static cold storage, were into...

Butyrate, a short-chain fatty acid, has shown potential to improve left ventricular (LV) function and induce vasorelaxation in rodents. Butyrate may either be produced by the microbiome colon, ingested or administered intravenously. This study aimed evaluate effects of butyrate on cardiac output (CO) associated hemodynamic variables porcine model. In randomized, blinded crossover study, ten healthy 60-kg pigs were given three hour infusions 600 mM equimolar sodium chloride (control). CO was...

Cardiogenic shock (CS) is characterized by reduced cardiac output (CO), end-organ perfusion, and high mortality. Medical therapies have failed to improve survival. The ketone body 3-hydroxybutyrate (3-OHB) enhances function in heart failure CS. We aimed elucidate the cardiovascular cardiometabolic effects of 3-OHB treatment during In a randomized, assessor-blinded crossover design, we studied 16 female pigs (60 kg, 5 months age). CS was induced left main coronary artery microsphere...

Abstract Background Butyrate, a short-chain fatty acid, has shown potential to improve left ventricular (LV) function and induce vasorelaxation in rodents. Butyrate may either be produced by the microbiome colon, ingested or administered intravenously. This study aimed evaluate effects of butyrate on cardiac output (CO) associated hemodynamic variables porcine model. Methods In randomized, blinded crossover study, ten healthy 60-kg pigs were given three-hour infusions 600 mM equimolar sodium...

Abstract Background Cardiogenic shock complicating myocardial infarction (AMI-CS) is charactirized by reduced cardiac output (CO), poor end-organ perfusion, and high mortality. Current medical therapies have not significantly improved survival rates. Lactate, though often considered a toxic byproduct of anaerobic metabolism, serves as an oxygen efficient alternative, readily oxidizable substrate for the myocardium. Lactate infusion has been shown to improve CO left ventricular ejection...

Background. Strategies to minimize ischemic damage during heart transplantation (HTX) by donation after circulatory death (DCD) are warranted because the inevitable injury linked DCD HTX deteriorates mitochondrial respiratory capacity and ultimately graft quality. This study aimed examine myocardial function with hypothermic oxygenated machine perfusion (HOPE) compare effect of normothermic regional (NRP) that direct procurement (DPP). Methods. A porcine model was used hearts subjected...

Abstract Animal models of cardiovascular disease are often evaluated by invasive instrumentation for phenotyping. As no consensus exists, both open‐ and closed‐chest approaches used, which might compromise rigour reproducibility in preclinical research. We aimed to quantify the cardiopulmonary changes induced sternotomy pericardiotomy a large animal model. Seven pigs were anaesthetized, mechanically ventilated right heart catheterization bi‐ventricular pressure–volume loop recordings at...

BACKGROUND: Insufficient fluid administration intra- and postoperatively may lead to delayed renal graft function (DGF), while overload increases the risk of heart failure, infection, obstipation. Several different protocols have been suggested ensure optimal state. However, there is a lack evidence clinical impact these regimens. This study aimed determine whether individualized goal-directed therapy (IGDT) positively affects initial compared high-volume (HVFT) examine effects on...

Thoracoabdominal normothermic regional perfusion (NRP) is a new method for in situ reperfusion and reanimation of potential donor organs donation after circulatory death by the thoracic abdominal with oxygenated blood. We investigated effects high oxygenation (HOX) versus low (LOX) during NRP on heart function porcine model.Pigs (80 kg) underwent 15-min anoxic cardiac arrest followed using heart-lung bypass machine subsequent assessment 180 min post-NRP. The animals were randomized to HOX...

The hemodynamic effects of aortic arch vessel (AAV) clamping during normothermic regional perfusion (NRP) in donation after circulatory death is unknown. We investigated AAV NRP compared with no a porcine model.In 16 pigs, parameters were recorded including biventricular pressure-volume measurements and invasive blood pressure. Additionally, gas inflammatory cytokines used to assess the effect clamping. animals centrally cannulated for NRP, baseline obtained before hypoxic arrest was induced...

Ex vivo lung perfusion (EVLP), is a platform that allows simultaneous testing and treatment of the lungs. However, use EVLP costly requires access to lab animals accompanying facilities. To increase for research, we developed method perform using abattoir procured Furthermore, were also able significantly decrease costs.Six pair lungs from sheep. The then flushed stored in ice 3 h. A low-flow (20% cardiac output) approach, tidal volume 6 ml/kg bodyweight total time h chosen. Perfusion fluids...

Background: Organs obtained from brain dead (BD) donors often have worse outcomes. Activation of the complement system and translocation intestinal bacteria could be causative. We aimed to examine activation following BD evaluate systemic local effect adding luminal preservation classical vascular preservation. Methods material: was induced in 30 pigs (four groups: control (n=7), alone (n=8), + polyethylene glycol (PEG, n=7) University Wisconsin solution (UW, n=8) using a previously...

The aim was to establish combined H

Organs obtained from brain dead donors can have suboptimal outcomes. Activation of the innate immune system and translocation intestinal bacteria could be causative. Thirty two pigs were assigned to control, death (BD), BD + luminal polyethylene glycol (PEG), University Wisconsin solution (UW) groups. Animals observed for 360 min after before organ retrieval. 2,000 mL preservation was instilled into duodenum at start procurement. Repeated measurements plasma C3a, Terminal Complement Complex...

<h3>BACKGROUND:</h3> Tracheostomy decannulation is accompanied by several clinical concerns due to air leakage. In this study, we introduced a novel tracheostoma closure device that facilitates the use of noninvasive ventilation, improvement pulmonary function, and vocalization in newly decannulated patient. The biosafety feasibility were evaluated an animal model. <h3>METHODS:</h3> Five Danish Landrace pigs subjected tracheostomy followed insertion device. Correct placement was ensured...

The Study is ongoing hence no conclusive results can be described objective of the study to investigate renal oxygenation and microcirculation in a high volume fluid therapy against an individualized goal directed (IGDT) during transplantation porcine model. Background A significant number kidney recipients experience complications after ranging from delayed graft function obstipation poor wound healing. Iatrogenic over hydration peri‐ postoperatively suspected culprit. Complications are...

Background Delayed Graft Function (DGF) is a common complication to kidney‐transplanted patients receiving kidney from brain dead donors. The present study designed evaluate the influence of fluid therapy on function and preservation glycocalyx, during transplantation using kidneys Method A porcine prospective randomized animal performed. recipients are either high volume or individualized goal directed (IGDT). pigs also no Nor‐epinephrine (NE) continuous low dose NE‐infusion. Brain death...

Abstract Background Lactate is traditionally acknowledged as a by-product of anaerobic glucose metabolism. Recently, however, lactate has been proven to be preferred oxidative substrate for stressed myocardium. Studies have shown that exogenous infusion provides beneficial hemodynamic effects including increased cardiac output (CO). Nevertheless, the exact mechanism action underlying not fully elucidated. Aim To identify cardiovascular mechanisms behind acute sodium in an experimental model...