A5088780024- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Bone Tissue Engineering Materials
- Proteoglycans and glycosaminoglycans research
- Surgical Sutures and Adhesives
- Wound Healing and Treatments
- Collagen: Extraction and Characterization
- Bacterial Genetics and Biotechnology
- Additive Manufacturing and 3D Printing Technologies
- Surgical site infection prevention
- Phenothiazines and Benzothiazines Synthesis and Activities
- biodegradable polymer synthesis and properties
- Diagnosis and Treatment of Venous Diseases
- Body Contouring and Surgery
- Animal Genetics and Reproduction
- Cancer therapeutics and mechanisms
Laboratoire de Biologie, Bioingéniérie et Bioimagerie ostéoarticulaire
2019-2022
Victoria University of Wellington
2009-2016
Macquarie University
2016
Ovine forestomach matrix (OFM) is a decellularized extracellular (dECM) biomaterial that serves as scaffold for remodeling damaged soft tissue. dECM biomaterials are used in variety of clinical applications, and their regenerative capacity encoded not only biophysical properties but also molecular diversity. In this study, the proteome OFM was characterized via both targeted global mass spectrometry (MS) with use heavy isotope labeled (SIL) internal standards. Proteins were identified...
Decellularized extracellular matrix (dECM)–based biomaterials are of great clinical utility in soft tissue repair applications due to their regenerative properties. Multi-layered dECM devices have been developed for indications where additional thickness and biomechanical performance required. However, traditional approaches the fabrication multi-layered introduce laminating materials or chemical modifications that may impair biological functionality material. Using an established...
ABSTRACT Venous leg ulcers (VLUs) are traditionally managed with standard‐of‐care dressings, compression and appropriate adjunctive venous interventions for pathologic reflux. Due to pathophysiological complexity underlying patient comorbidities, conducting randomised controlled trials evaluate the comparative efficacy of advanced treatment modalities is difficult, as many patients would likely be excluded. This retrospective, pragmatic, real‐world evidence (RWE) study compared healing...
ABSTRACT In this study, application of a dual absorbance/fluorescence assay to chemical library screen identified several previously unknown inhibitors mycobacteria. addition, growth conditions had significant effect on the activity profile library. Some such as Se-methylselenocysteine were detected only when screening was performed under nutrient-limited culture opposed nutrient-rich conditions. We propose that multiple condition is required for complete inhibitory profiling and maximal...
Abstract Ovine forestomach matrix (OFM) is a native and functional decellularized extracellular biomaterial that supports cell adhesion proliferation remodeled during the course of tissue regeneration. Small angle X‐ray scattering demonstrated OFM retains collagen architecture ( d spacing = 63.5 ± 0.2 nm, orientation index 20°). The biophysical properties were further defined using ball‐burst, uniaxial suture retention testing, as well quantification aqueous permeability. was relatively...
Scaffold biomaterials are typically applied surgically as reinforcement for weakened or damaged tissue, acting substrates on which healing tissue can grow. Natural extracellular matrix (ECM) materials consisting mainly of collagen often used this purpose, but anisotropic. Ovine forestomach (OFM) ECM was exposed to increasing strain and synchrotron-based SAXS diffraction patterns revealed that the fibrils within underwent changes in orientation, orientation index (a measure isotropy),...
Soft tissue is composed of cells surrounded by an extracellular matrix that made up a diverse array intricately organized proteins. These distinct components work in concert to maintain homeostasis and respond damage. During repair, proteins their degradation products are known influence physiological processes such as angiogenesis inflammation. In this study we developed discovery platform using decellularized biomaterial identify new chemotrophic factors derived from the matrix. An vitro...
Decellularized extracellular matrix (dECM) products are widely established for soft tissue repair, reconstruction and reinforcement. These regenerative biomaterials mimic native ECM with respect to structure biology produced from a range of sources species. Optimal source processing requires balance between removal cellular material the preservation structural biological properties ECM. Despite wide-spread clinical use dECM there is lack comparative information on these Structurally, some...
Antimicrobial technologies, including silver-containing medical devices, are increasingly utilized in clinical regimens to mitigate risks of microbial colonization. Silver-functionalized resorbable biomaterials for use wound management and tissue regeneration applications have a narrow therapeutic index where antimicrobial effectiveness may be outweighed by adverse cytotoxicity. We examined the effects ionic silver functionalization an extracellular matrix (ECM) biomaterial derived from...
Biomaterials and negative pressure wound therapy (NPWT) are treatment modalities regularly used together to accelerate soft-tissue regeneration. This study evaluated the impact of design composition commercially available collagen-based matrices on observed vacuum delivered under NPWT using a custom test apparatus. Specifically, testing compared effect commercial products; ovine forestomach matrix (OFM), collagen/oxidized regenerated cellulose (collagen/ORC) dressing (CWD) pressure. OFM...
Biomaterials and negative pressure wound therapy (NPWT1) are treatment modalities regularly used together to accelerate soft-tissue regeneration. This study evaluated the impact of design composition commercially available collagen-based matrices on observed vacuum delivered under NPWT using a custom test apparatus. Specifically, testing compared effect commercial products; ovine forestomach matrix (OFM2), collagen/ORC dressing (CWD3) pressure. OFM resulted in an ~50% reduction target at 75...