A5101911250- Viral Infectious Diseases and Gene Expression in Insects
- Microbial Natural Products and Biosynthesis
- Protein purification and stability
- Monoclonal and Polyclonal Antibodies Research
- Assisted Reproductive Technology and Twin Pregnancy
- Enzyme Structure and Function
- Carbohydrate Chemistry and Synthesis
- RNA and protein synthesis mechanisms
- Birth, Development, and Health
- Demographic Trends and Gender Preferences
- Biochemical and Molecular Research
- Cognitive Abilities and Testing
- Chemical Synthesis and Analysis
- Occupational and environmental lung diseases
- Metal-Catalyzed Oxygenation Mechanisms
- Fungal Biology and Applications
- Fluid Dynamics and Mixing
- Chemical synthesis and alkaloids
- Phytochemical compounds biological activities
- Virus-based gene therapy research
- Microfluidic and Capillary Electrophoresis Applications
- Fungal and yeast genetics research
- Antibiotic Resistance in Bacteria
- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
Tokyo Women's Medical University
2024-2025
Daiichi-Sankyo (Japan)
2012-2024
Daiichi-Sankyo (South Korea)
2009-2024
Cell Biotech (South Korea)
2023
Biocat
2023
Wako University
2001-2016
Daiichi Sankyo (Germany)
2009-2016
Gunma Astronomical Observatory
2016
Pharmaceutical Biotechnology (Czechia)
2012
Daiichi Sankyo (United Kingdom)
2012
The lipopeptidyl nucleoside antibiotics represented by A-90289, caprazamycin, and muraymycin are structurally highlighted a core that contains nonproteinogenic β-hydroxy-α-amino acid named 5′-C-glycyluridine (GlyU). Bioinformatic analysis of the biosynthetic gene clusters revealed shared open reading frame encoding protein with sequence similarity to serine hydroxymethyltransferases, resulting in proposal this enzyme catalyzes an aldol-type condensation glycine uridine-5′-aldehyde furnish...
Lip reading: The biosynthetic gene cluster for A-90289, a fatty acid nucleoside antibiotic, was cloned and sequenced. sulfotransferase LipB is demonstrated to be essential A-90289 biosynthesis, in vitro characterization revealed utilizes p-nitrophenylsulfate as an aryl sulfate donor variety of nonaryl acceptors including caprazamycin A give the 2′-O-sulfated product, A-90289A. Enzymes involved peptidoglycan cell wall biosynthesis are proven targets antibacterial drugs that have...
Fe(II)- and α-ketoglutarate (α-KG)-dependent dioxygenases are a large diverse superfamily of mononuclear, non-heme enzymes that perform variety oxidative transformations typically coupling decarboxylation α-KG with hydroxylation prime substrate. The biosynthetic gene clusters for several nucleoside antibiotics contain modified uridine component, including the lipopeptidyl A-90289 from Streptomyces sp. SANK 60405, have recently been reported, revealing shared open reading frame sequence...
Abstract Recombinant protein production technology is widely applied to the manufacture of biologics used as drug substances and industrial proteins such recombinant enzymes bioactive proteins. Various heterologous systems have been developed using prokaryotic eukaryotic hosts. Especially methylotrophic yeast in hosts suggested be particularly valuable because following advantages: secretion into culture broth, quality control systems, a post-translational modification system, rapid growth,...
Abstract The monozygotic (MZ) twinning rate in Japan had remained nearly constant from 1975 (3.74 per 1,000 births) to 1994 (4.23), whereas the dizygotic (DZ) (1.86) 1986 (2.27), and gradually increased up (3.89). higher DZ since 1987 has been attributed proportion of mothers treated with ovulation-inducing hormones partially in-vitro fertilisation Japan. As for maternal age, MZ rates have age groups except youngest oldest groups. On other hand, 35-39 years group decrease thereafter. In...
Chinese hamster ovary (CHO) cells are widely utilized in the production of antibody drugs. To ensure large quantities antibodies that meet required specifications, it is crucial to monitor and control levels metabolites comprehensively during CHO cell culture. In recent years, continuous analysis methods employing on-line/in-line techniques using Raman spectroscopy have attracted attention. While these analytical can nondestructively culture data, constructing a highly accurate measurement...
Several peptidyl nucleoside antibiotics that inhibit bacterial translocase I involved in peptidoglycan cell wall biosynthesis contain an aminoribosyl moiety, unusual sugar appendage natural products. We present here the delineation of biosynthetic pathway for this moiety upon vitro characterization four enzymes (LipM-P) are functionally assigned as (i) LipO, L-methionine:uridine-5'-aldehyde aminotransferase; (ii) LipP, a 5'-amino-5'-deoxyuridine phosphorylase; (iii) LipM,...
A-500359s, A-503083s, and A-102395 are capuramycin-type nucleoside antibiotics that were discovered using a screen to identify inhibitors of bacterial translocase I, an essential enzyme in peptidoglycan cell wall biosynthesis. Like the parent capuramycin, A-500359s A-503083s consist three structural components: uridine-5′-carboxamide (CarU), rare unsaturated hexuronic acid, aminocaprolactam, last which is substituted by unusual arylamine-containing polyamide A-102395. The biosynthetic gene...
Papilledema is optic disc swelling due to intracranial hypertension, which leads progressive visual impairment. We report a rare case of papilledema with ectopic orbital calcification during hemodialysis. A 34-year-old woman low body weight who was undergoing long-term hemodialysis presented in both eyes, and her vision gradually deteriorated over the course six months. The best corrected acuity 20/16 right eye 20/500 left eye, fundus examination revealed significant field testing nasal...
We conducted a comparative study of pulmonary dysfunction among workers who were exposed to silica, asbestos, or coalmine dust. The results showed that all three groups dust-exposed workers, even those without radiographic signs pneumoconiosis, had decreased spirometric parameters and diffusing capacity (DLco) in both nonsmokers smokers. Pulmonary function was further when pneumoconioses present the groups. In accord with increasing categories, workers' pneumoconiosis (CWP), it changed...
ABSTRACT When antibodies were expressed in the methylotrophic yeast Ogataea minuta , we found that abnormal O mannosylation occurred secreted antibody. Yeast-specific is initiated by addition of mannose at serine (Ser) or threonine (Thr) residues endoplasmic reticulum via protein mannosyltransferase (Pmt) activity. To suppress O-linked sugar chains on antibodies, examined possibility inhibiting Pmt activity a inhibitor during cultivation. The was to partially antibodies. Surprisingly,...
Rise and shine: Using a gene-targeting approach aimed at identifying potential L-threonine:uridine-5′-transaldolases that catalyze the formation of (5′S,6′S)-C-glycyluridine, new bacterial translocase I inhibitor was discovered from an actinomycete following fermentation optimization.
Contrary to intuitive preconceptions, patients with Parkinson’s disease (PD) have been reported less carious teeth. The present study was undertaken seek responsible factors for this unexpected finding. PD consisted of 31 consecutive university hospital outpatients who were 60 years old or over, and the controls 104 comparable at a dental clinic. They inspected their status, interviewed on toothbrushing habits dietary preferences. Their unstimulated saliva samples collected flow volume pH...
Using the ATP-independent transacylase CapW required for biosynthesis of capuramycin-type antibiotics, we developed a biocatalytic approach synthesis 43 analogues via one-step aminolysis reaction from methyl ester precursor as an acyl donor and various nonnative amines acceptors. Further examination substrate scope revealed that this enzyme can also catalyze direct transamidation using major capuramycin congener semisynthetic precursor. Biological activity tests few new have significantly...
Muraminomicin is a lipopeptidyl nucleoside antibiotic produced by Streptosporangium amethystogenes SANK 60709. Similar to several members of this family such as A-90289 and muraymycin, the structure muraminomicin consists disaccharide comprised two modified ribofuranose units linked an O-β(1 → 5) glycosidic bond; however, holds distinction in that both ribose are 2-deoxy sugars. The biosynthetic gene cluster has been identified, cloned sequenced, bioinformatic analysis revealed minimum 24...