Doxorubicin (DXR) is used in breast cancer therapy, and may change transcriptional profile of cells.Tristetraprolin (TTP) an mRNA-decay protein, which downregulated cancer, inhibits invasion suggested as a positive prognostic marker.Migration require the presence TKS4, TKS5, N-WASP, WIP CTTN proteins.The investigation how doxorubicin regulate these targets important for understanding its impact on tumor.Aim.The study aims to investigate effect treatment MCF7 cells expression ZFP36, SH3PXD2A,...

TKS4 scaffold protein is involved in formation of invadopodia, production reactive oxygen species (ROS) by tumor cells and other cellular processes.Aim.To identify new partners the actin cytoskeleton rearrangements endo-/exocytosis.Methods.The GST pull-down assay was used to interaction.Results.We revealed that SH3 domains interacted with reorganization proteins N-WASP CR16, as well DNM2, SYNJ1 OPHN1 which are endo-/ exocytosis.We also tested WIP, WIRE, SHIP2, RhoU, RhoV NUMB proteins, but...

Aim. Intersectin 1 (ITSN1) gene encodes an evolutionarily conserved adaptor protein that functions in clathrin-mediated endocytosis, cell signalling, apoptosis and cytoskeleton rearrangements.Its expression is characterized by multiple alternative splicing.Alternative promoter usage additional way to create diversity flexibility the regulation of expression.The aim this study was identify possible promoters ITSN1 gene.Methods.In silico prediction, 5¢ RACE, RT-PCR reporter assay were used for...

ABSTRACT Rho GTPases play a key role in various membrane trafficking processes. RhoU is an atypical small GTPase related to Rac/Cdc42, which possesses unique N- and C-terminal domains that regulate its function subcellular localization. localizes at the plasma membrane, on endosomes cell adhesion structures where it governs signaling, differentiation migration. However, despite endomembrane localization, vesicular has been unexplored. Here, we identified intersectins (ITSNs) as new binding...

Adaptor/scaffold proteins of the intersectin (ITSN) family are important components endocytic and signalling complexes.They coordinate trafficking events with actin cytoskeleton rearrangements modulate activity a variety pathways.In this review, we present our results as part recent findings on function ITSNs, role alternative splicing in generation ITSN1 diversity potential relevance ITSNs for neurodegenerative diseases cancer.

²íòåðñåêòèí 1 (ITSN1) -áàãàòîôóíêö³îíàëüíèé àäàïòîðíèé á³ëîê, ùî áåðå ó÷àñòü â åíäîöèòîç³, åêçî-öèòîç³ òà ïåðåäà÷³ êë³òèííèõ ñèãíàë³â, à òàêîae àñîö³éîâàíèé ç òàêèìè ïàòîëîã³ÿìè, ÿê ñèíäðîì Äàóíà ³ õâîðîáà Àëüöãåéìåðà.Ìåòà.Âèÿâëåííÿ íîâèõ á³ëêîâèõ ïàðòíåð³â ITSN1, çàëó÷åíèõ äî ïðîöåñ³â ìåìá

TKS5 is a scaffold protein that takes part in invadopodia functioning and reactive oxygen species (ROS) production.TKS5 critical component of as its absence results the loss cancer cells ability to form these invasive structures.TKS5 phosphorylated by SRC kinase consequently interacts with membrane phosphatidylinositol phosphates launching formation process.At later stages regulates actin cytoskeleton reorganization extracellular matrix degradation.TKS5 also production ROS, which are...

ITSN1 adaptor/scaffold protein takes part in a variety of physiological and pathological cellular processes.It has complex expression regulation many partners.Aim.Characterization the functio ning control is important for understanding its role cell.Methods.Bioinformatic analysis, semiquantitative analysis by RTPCR, immunoprecipitation.Results.We have described ana lyzed promoter regions, detected alternatively spliced isoforms at mRNA levels different cancer specimens.Using bioinformatic...

Aim. Regardless a great progress in treatment, 15 % of breast cancer cases remain lethal.One the main problems diagnosis and cure this type is its high clinical genetic heterogeneity, identification markers for personalized treatment still topical issue.Methods.Collection characterization material, RNA isolation analysis ITSN2 TKS5 isoforms expression using real-time quantitative PCR with fluorescence labeled probes.Results.The reduced ITSN2-S has been found HER2/neupositive tumors poor...

ITSN1 is an endocytic scaffold protein with a prominent function in synaptic transmission.It known that Ca 2+ signaling crucial for the regulation of proteins functioning.Aim.Checking possibility /calmodulin-dependent phosphorylation ITSN1.Methods.Affinity chromatography, vitro kinase reaction, Western blotting, gel staining fluorescent stains.Results.We show fraction calmodulin-binding able to phosphorylate recombinant fragments encoding coiled-coil region and SH3 domain-containing presence...

RNA-binding RAS-GTPase G3BP1 is a multi-functional protein that known to be one of the key components stress granules -non-membrane ribonucleoprotein cell compartments are formed by liquid-liquid phase separation.With its ability mediate both protein-protein and protein-RNA interactions, perceived as regulator granule assembly.Aim.Explore influence domains consequence SGassociated RNA binding interaction on mRNA recruitment assembly.Methods.Molecular cloning, Microtubule-bench assay coupled...

Abstract Tristetraprolin (TTP) is an RNA‐binding protein that negatively regulates its target mRNAs and has been shown to inhibit tumor progression invasion. Tumor invasion requires precise regulation of cytoskeletal components, dysregulation cytoskeleton‐associated genes can significantly alter cell motility invasive capability. Several genes, including SH3PXD2A, SH3PXD2B , CTTN WIPF1 WASL are crucial components the cytoskeleton reorganization machinery essential for adequate motility....

ZFP36 is a gene which downregulated in various malignancies, and its expression level often considered as potential prognostic diagnostic mar ker.Aim.In current study we investigated how the varies breast tumors of different histological types, grades, metastatic status, well with progesterone human EGFR receptor 2 (HER2) status to assess use cancer biomarker.Methods.RT-qPCR.Results.We show that elevated T1-2 N0-N1 compared adjacent tissues T3-4 N2 tumors, respectively.We also does not vary...

• TTP expression level differs in different breast cancer subtypes. HER2-enriched samples have the highest of expression. Temozolomid and doxorubicin influence TTPs methylation status.

Intersectin 1 (ITSN1) is an adaptor protein involved in membrane trafficking and cell signaling.Long short isoforms of ITSN1 (ITSN1-L ITSN1-S) are produced by alternative splicing.The aim our study was to investigate whether ITSN1-L ITSN1-S could interact mammalian cells.Methods.During this we employed immunoprecipitation confocal microscopy.Results.We have shown that endogenous coprecipitates with overexpressed PC12, 293 293T cells.Long also colocalize cells.Conclusions.ITSN1-L form complexes cells.

The loop domain organization of chromatin, which plays an important role in transcription regulation, may depend on the cell functional state. aim this work was to investigate DNA reorganization upon transitions two lines ‒ glioblastoma multiforme T98G and astrocytoma U373.Single gel electrophoresis (the comet assay) used analyze kinetics migration from nucleoids obtained lysed cells.The fraction surface loops size these were found be similar lines. When synthetic processes inhibited, a...

Aim. Despite the great progress in cancer treating, breast remains lethal 15 % cases. Regardless of many years research and extensive experience treatment this type cancer, one main problems diagnosis therapy is its high clinical genetic heterogeneity. Thereby identification markers for personalized patients still an actual issue. Methods. Collection material, RNA isolation, expression analysis ITSN2 TKS5 isoforms using quantitative real time PCR with fluorescence-labeled probes. Results. We...