The rate of superoxide anion radical, hydroxyl radical and hydrogen peroxide generation, the level oxidative modification mitochondrial proteins in liver rats with toxic hepatitis was investigated on background alimentary protein deficiency. We did not find significant increases intensity free processes mitochondria maintained protein-deficient ration. most intensification is observed under conditions hepatitis, induced deprivation. Under these aggravation all studied forms reactive oxygen...

The activity of detoxication enzymes in liver microsomal fraction preliminary radiation-exposed rats was investigated. It shown that organism exposure to radiation reduced cytochrome Р450 and glutathione-S-transferase the latent logarithmic phases oncogenesis compared with unirradiated tumor.Low level can be caused by transition P450 P420 inactive form.The does not influence enzyme on terminal stages Guerin's carcinoma growth.

Activity of isocitrate dehydrogenase, α-ketoglutarate malate and the NAD(+)/NADН ratio were studied in liver mitochondrial fraction rats with toxic hepatitis induced by acetaminophen under conditions alimentary protein deprivation. Acetaminophen-induced was characterized a decrease dehydrogenase activities, while remained at control level. Modeling acetaminophen-induced caused more pronounced activity NAD(+)-dependent dehydrogenases 2.2-fold increase ratio. This suggests that deprivation...

Background. Acetaminophen is known as inducer of acute hepatotoxicity. Extrahepatic manifestations acetaminophen toxicity are poorly understood in particular its nephrotoxicity. Objective. The purpose this study was the morphological characteristic rat kidneys under conditions acetaminophen-induced nephrotoxicity on background alimentary deprivation protein. Methods. Аfter administration toxic dose and maintenance rats a different regimen protein nutrition their were sectioned stained with...

The research deals with the determination of activity aldehyde dehydrogenase (Ec 1.2.1.3),aldehyde reductase 1.1.1.21)as well as content TBa reactive substances and protein carbonyl derivates in rat liver cytosolic fraction under conditions acetaminophen-induced hepatitis deficiency.The most pronounced decrease enzymes utilizing endogenous aldehydes is observed animals toxic injury maintained alimentary deficiency.Meanwhile, accumulation TBA carbonyl-derivates this experimental group was...

The activity of free radical processes in liver mitochondria was investigated rats kept on high-sucrose and low protein/high-sucrose diets. Excess dietary sucrose caused intensification as evidenced by increased hydroxyl generation, accumulation primary (conjugated dienes, ketodienes) secondary products (TBA-reactive products) lipid peroxidation, cholesterol/phospholipids ratio also oxidative modification proteins (carbonyl derivatives). Additional nutritional protein deficiency (low diet)...

The research deals with the integral haematological indices such as markers of immunoreactivity and phagocytic activity neutrophils in animals kept conditions a nutrient imbalance. were divided into four experimental groups: I — receiving full-value semi-synthetic ration (control group); II low-protein (LPR); III high-sucrose diet (HS); IV low-protein/high-sucrose (LPR/HS). It has been found that nutritional protein deficiency there was disturbance specific immune response, evidenced by...

Метою роботи було дослідження активності маркерних ензимів стану печінки щурів за умов ацетамінофен-індукованого токсичного ураження та впливу цитрату германію.Для досягнення мети у роботі проведена оцінка сорбітолдегідрогеназної, аланін-та аспартатамінотрансферазної сироватці крові, а також розраховано коефіцієнт де Рітіса досліджуваних

Aim.The features of the energy supply in hepatocytes rats under conditions different content protein and sucrose a diet were studied research.Methods.Experimental diets, differential centrifugation, spectrophotometry, chromatography on sheets.Results.It was found that 4-week maintenance animals low-protein resulted 2.2-fold decrease succinate dehydrogenase activity, threefold reduction cytochrome oxidase an increase F0F1-ATPase activity 1.5-fold rats.Under experimental conditions, 40 % ATP...

The study of mechanisms the metabolic disorders in conditions deficiency or excess individual nutrients diet is a live issue. influence simultaneous sucrose intake and protein on functional state liver remains poorly understood. aim research was to rate generation superoxide radicals, content triglycerides glycogen liver, as well activity enzymatic markers rats fed diets with different content. Material methods. studies were conducted over 28 days 48 white non-linear rats, randomized into 4...

The relationship between the quantitative ratio of redox forms ubiquinone and degree free radical damage to mitochondrial proteins in rat liver against background nutritional imbalance was investigated. animals were divided into following experimental groups: I – receiving full-value semi-synthetic ration (control group); II high-sucrose diet; III low-protein diet. content total oxidized determined spectrophotometrically at 275 nm, reduced by difference ubiquinone. intensity oxidative...

p-Hydroxylase and N-demethylase activities of cytochrome P450 system, NO-synthase activity the intensity nitric oxide superoxide anion production in mitochondrial, postmicrosomal microsomal cellular fractions were studied mouse liver under conditions retinoid stores absence.It is determined, that absence activation occurring with decreased p-hydroxylase system. The results generation analysis showed level NO О2- mitochondrial fraction knock-out mice, changes NADPH-dependent cells.