A5086463195- Heme Oxygenase-1 and Carbon Monoxide
- Pluripotent Stem Cells Research
- Spectroscopy Techniques in Biomedical and Chemical Research
- Cancer, Hypoxia, and Metabolism
- CRISPR and Genetic Engineering
- Pancreatic function and diabetes
- Congenital heart defects research
- Alzheimer's disease research and treatments
- Neonatal Health and Biochemistry
- Hematopoietic Stem Cell Transplantation
- Immune cells in cancer
- Aquaculture disease management and microbiota
- Sarcoidosis and Beryllium Toxicity Research
- ATP Synthase and ATPases Research
- Hemoglobin structure and function
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Cancer-related Molecular Pathways
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Autophagy in Disease and Therapy
- Epigenetics and DNA Methylation
- Immune Cell Function and Interaction
- Genetics and Neurodevelopmental Disorders
- Spinal Cord Injury Research
- Biomarkers in Disease Mechanisms
- Cancer Immunotherapy and Biomarkers
Jagiellonian University
2015-2024
International Institute of Molecular and Cell Biology
2009-2010
Polish Academy of Sciences
2006-2008
Wageningen University & Research
2006
In this work, 3D linear Raman spectroscopy was used to study lipid droplets (LDs) ex vivo in liver tissue and also vitro a single endothelial cell. Spectroscopic measurements combined with fluorescence microscopy and/or histochemical staining gave complex chemical information about LD composition enabled detailed investigations of the changes occurring various pathological states. Lipid analysis fatty performed using dietary mouse model steatosis, induced by high fat diet (HFD). HFD is...
Raman imaging was used to illustrate heterogeneity of a single endothelial cell and the vascular wall sample. The spectral analysis allowed for exploring complexity studied systems in three dimensions defining size, volume, shape biochemical composition cellular organelles. ability construct 3D maps by method that does not disrupt spatial integrity provided unique insight into architecture processes endothelium wall. may be considered as new trend analytical spectroscopy applied biomedical...
Abstract Mouse and human induced pluripotent stem cells (iPSCs) may represent a novel approach for modeling diabetes. Taking this into consideration, the aim of study was to generate evaluate differentiation potential iPSCs from lep db/db (db/db) mice, model diabetes type 2 as well patients with Maturity Onset Diabetes Young 3 (HNF1A MODY). Murine iPSC colonies both wild mice were positive markers pluripotency: Oct3/4A, Nanog, SSEA1, CDy1 alkaline phosphatase differentiated in vitro vivo...
USP2a is a deubiquitinase responsible for stabilization of cyclin D1, crucial regulator cell-cycle progression and proto-oncoprotein overexpressed in numerous cancer types. Here we report that lithocholic acid (LCA) derivatives are inhibitors USP proteins, including USP2a. The most potent LCA derivative, hydroxyamide (LCAHA), inhibits USP2a, leading to significant Akt/GSK3β-independent destabilization but does not change the expression p27. This leads defects progression. As result, LCAHA...
Burkitt lymphoma (BL) is a rapidly growing tumor, characterized by high anabolic requirements. The MYC oncogene plays central role in the pathogenesis of this malignancy, controlling genes involved apoptosis, proliferation, and cellular metabolism. Serine biosynthesis pathway (SBP) couples glycolysis to folate methionine cycles, supporting certain amino acids, nucleotides, glutathione, methyl group donor, S-adenosylmethionine (SAM). We report that BLs overexpress SBP enzymes,...
Tumor hypoxia and high activity of hypoxia-inducible factor-1 (HIF-1) correlate with adverse disease outcomes, malignancy, resistance to therapy metastasis. Nonetheless, recent studies indicate that under certain circumstances, HIF-1 stabilization may exert protective effects even decrease tumor cell aggressiveness. This study aimed characterize the potential anticancer effect molidustat (BAY 85-3934), prolyl hydroxylase (PHD) inhibitor stabilizator. We confirmed stabilizes HIF-1α induces...
Murine very small embryonic-like (VSEL) cells, defined by the Lin−Sca-1+CD45− phenotype and size, were described as pluripotent cells proposed to be most primitive hematopoietic precursors in adult bone marrow. Although their isolation potential application rely entirely on flow cytometry, immunophenotype of VSELs has not been extensively characterized. Our aim was analyze possible heterogeneity Lin−Sca+CD45− population investigate extent which characteristics may overlap with that stem...
Cell therapies are extensively tested to restore heart function after myocardial infarction (MI). Survival of any cell type intracardiac administration, however, may be limited due unfavorable conditions damaged tissue. Therefore, the aim this study was evaluate therapeutic effect adipose-derived stromal cells (ADSCs) and human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) overexpressing either proangiogenic SDF-1α or anti-inflammatory heme oxygenase-1 (HO-1) in a murine...
Degradation during production and delivery is a significant bottleneck in developing biomolecular therapies. Here, we show that encapsulation protein cages formed by engineered variants of cage-forming lumazine synthase establishes an effective route for microbial isolation otherwise difficult-to-express, degradation-prone polypeptides. In this system, genetic fusion to cage component protomer ensures efficient guest packaging while being produced host bacterial cells. Meanwhile, the...
Supravital species identification of morphologically similar syntopic earthworms inhabiting dung and compost heaps or those from commercial cultures is difficult. The aim the studies was to find out non-invasive species-specific markers for proper segregation earthworm a dense mixed colony waste decomposers. Worms were segregated according external characteristics into Eisenia andrei, fetida, Dendrobaena veneta, left reproduction analysis non-invasively retrieved coelomocyte-containing...
Abstract While intrinsic changes in aging hematopoietic stem cells (HSCs) are well characterized, it remains unclear how extrinsic factors affect HSC aging. Here, we demonstrate that the niche—endothelial (ECs) and CXCL12‐abundant reticular (CARs)—highly express heme‐degrading enzyme, heme oxygenase 1 (HO‐1), but then decrease its expression with age. HO‐1‐deficient animals (HO‐1 −/− ) have altered numbers of ECs CARs produce less factors. HSCs co‐cultured vitro HO‐1 mesenchymal stromal...
Abstract The nuclear factor erythroid 2-related 2 (Nrf2) is considered as a master cytoprotective regulating the expression of genes encoding anti-oxidant, anti-inflammatory, and detoxifying proteins. role Nrf2 in pathophysiology skeletal muscles has been evaluated different experimental models, however, due to inconsistent data, we aimed investigate how transcriptional deficiency (Nrf2 tKO ) affects muscle functions both an acute chronic injury. damage was induced mice two genotypes—WT by...
Inhibition of heme oxygenase-1 (HO-1, encoded by HMOX1), a cytoprotective, anti-apoptotic and anti-inflammatory enzyme, may be valuable therapy in various pathophysiological processes, including tumorigenesis. We compared the effect chemical inhibitors – metalloporphyrins, with genetic tools - shRNA CRISPR/Cas9 systems, to knock-down (KD)/knock-out (KO) HO-1 expression/activity. 293T cells were incubated tin zinc protoporphyrins (SnPPIX ZnPPIX, respectively) or either transduced lentiviral...
Patients with HNF1A-maturity-onset diabetes of the young (MODY) often develop endothelial dysfunction and related microvascular complications, like retinopathy. As clinical phenotype HNF1A-MODY varies considerably, we used human induced pluripotent stem cells (hiPSCs) from two healthy individuals (control) to generate isogenic lines mutation in HNF1A gene. Subsequently, control hiPSCs their respective clones were differentiated toward (hiPSC-ECs) different markers/functions compared. Human...
Mesenchymal stromal cells (MSCs) are heterogeneous from adult tissues that able to differentiate in vitro into adipocytes, osteoblasts, or chondrocytes. Such widely studied regenerative medicine. However, the success of cellular therapy depends on cell survival. Heme oxygenase-1 (HO-1, encoded by Hmox1 gene), an enzyme converting heme biliverdin, carbon monoxide, and Fe2+, is cytoprotective can affect stem performance. Therefore, our study aimed at assessing whether critical for survival...
Abstract Cellular stress can influence efficiency of iPSCs generation and their differentiation. However, the role intracellular cytoprotective factors in these processes is still not well known. Therefore, we investigated effect HO‐1 ( Hmox1 ) or Nrf2 Nfe2l2 ), two major genes. –/– fibroblasts demonstrated decreased reprogramming comparison to +/+ cells. Reversely, pharmacological enhancement resulted higher number colonies. Importantly, elevated level both p53 p53‐regulated miR‐34a 14‐3‐3σ...
Article11 November 2019Open Access Transparent process Cobalt protoporphyrin IX increases endogenous G-CSF and mobilizes HSC granulocytes to the blood Agata Szade Corresponding Author [email protected] orcid.org/0000-0002-0575-4659 Department of Medical Biotechnology, Faculty Biochemistry, Biophysics Jagiellonian University, Krakow, Poland Search for more papers by this author Krzysztof orcid.org/0000-0002-7227-4276 Witold N Nowak orcid.org/0000-0002-1526-3511 Karolina Bukowska-Strakova...